Thermo Scientific Pierce CDI-Activated Agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilization of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.
Pierce CDI-activated Resin is porous, beaded agarose chromatography resin that is activated with carbonyl diimidazole groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles. Coupling can be performed in aqueous or organic solvent conditions, making possible the immobilization of many kinds of ligands. Once a molecule is immobilized, the prepared affinity resin can be used multiple times in typical affinity purification procedures.
- Reliable coupling chemistry – immobilization occurs through the reaction of N-nucleophiles with 1,1' -carbonyldiimidazole groups of the resin to form a stable, uncharged N-alkylcarbamate linkages
- Easy-to-use – no secondary reagents needed; just wash equilibrate resin in alkaline coupling buffer and add ligand; reaction proceeds spontaneously
- Stable activation – half-life of hydrolysis is much longer than hydroxysuccinimide ester activations, making immobilization reactions simpler to prepare and control; simplifies filtration and washing before adding a ligand or protein
- Well-defined coupling conditions – reaction is most efficient with primary amines at pH 9 to 11
Properties of CDI-activated Agarose:
- Resin – crosslinked 6% beaded agarose
- Highly activated – at least 50µmol 1,1'-carbonyldiimidazole (CDI) groups per milliliter of resin
- Convenient form – supplied as stabilized, 50% slurry in acetone
- Shenoy, S.K., et al. (2001). Science 294: 1307-1313.
- Richardson, R.T., et al. (2000). J. Biol. Chem. 275: 30378-30386.
- Tanaka, M, et al. (2005). PLoS Biology 3:764-776.
Review of affinity purification
Review of covalent immobilization methods
All Affinity Supports for Ligand Immobilization