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Pierce CDI-activated Agarose

Carbonyldiimidazole-activated agarose for ligand immobilization.

CDI-activated Resin

Thermo Scientific Pierce CDI-Activated Agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilization of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

Pierce CDI-activated Resin is porous, beaded agarose chromatography resin that is activated with carbonyl diimidazole groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles. Coupling can be performed in aqueous or organic solvent conditions, making possible the immobilization of many kinds of ligands. Once a molecule is immobilized, the prepared affinity resin can be used multiple times in typical affinity purification procedures.


  • Reliable coupling chemistry – immobilization occurs through the reaction of N-nucleophiles with 1,1' -carbonyldiimidazole groups of the resin to form a stable, uncharged N-alkylcarbamate linkages
  • Easy-to-use – no secondary reagents needed; just wash equilibrate resin in alkaline coupling buffer and add ligand; reaction proceeds spontaneously
  • Stable activation – half-life of hydrolysis is much longer than hydroxysuccinimide ester activations, making immobilization reactions simpler to prepare and control; simplifies filtration and washing before adding a ligand or protein
  • Well-defined coupling conditions – reaction is most efficient with primary amines at pH 9 to 11

Product Details:

Coupling chemistry of Pierce CDI-activated Agarose
Immobilization chemistry of Thermo Scientific Pierce CDI-activated Agarose. Learn more about amine-reactive immobilization supports.

Properties of CDI-activated Agarose:

  • Resin – crosslinked 6% beaded agarose
  • Highly activated – at least 50µmol 1,1'-carbonyldiimidazole (CDI) groups per milliliter of resin
  • Convenient form – supplied as stabilized, 50% slurry in acetone


  1. Shenoy, S.K., et al. (2001). Science 294: 1307-1313.
  2. Richardson, R.T., et al. (2000). J. Biol. Chem. 275: 30378-30386.
  3. Tanaka, M, et al. (2005). PLoS Biology 3:764-776.

Related Resources:

Review of affinity purification
Review of covalent immobilization methods

Related Products:

All Affinity Supports for Ligand Immobilization

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
20259 Pierce CDI-activated Agarose Resin
Formulation: 1,1'-carbonyldiimidazole activated crosslinked 6% beaded agarose, slurried in acetone
Sufficient For: Immobilizing amine ligands to resin having > 50┬Ámol CDI-reactive groups per mL
10mL Product Instructions for product #20259 Pierce CDI-activated Agarose Resin MSDS for product #20259 Pierce CDI-activated Agarose Resin Certificate of Analysis for product #20259 Pierce CDI-activated Agarose Resin $93.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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