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Carboxy-PEG-Amine Compounds


Discrete-length polyethylene glycol (pegylated) amino acids.

Pierce CA(PEG)n Compounds

Thermo Scientific Pierce CA(PEG)n Compounds are pegylated amino acids of the form carboxy-PEG-amine, where n equals 4 to 24 polyethylene glycol units, and are useful for a variety of surface- and molecule-pegylation applications.

CA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers with carboxy (-COOH) and amino (-NH2) termini. The unbranched, hydrophilic, discrete-length molecules have the form Carboxy-PEGn-Amine, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The carboxyl and primary amine of each compound provide specific targets for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.

Highlights:

  • Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
  • Provided as a series of 4, 8, 12 and 24 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
  • PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
  • Allows site-specific labeling or crosslinking involving primary amines or carboxyl groups on proteins or surfaces
  • Easy-to-follow instructions increase the likelihood of a successful outcome

Product Details:

CA(PEG)n Compounds
Chemical structure of Carboxy-PEG-Amine compounds, CA(PEG)n. Alternative names include Amino-dPEG-acid, Amino-PEG-carboxylic acid, and Carboxy-PEO-amine.

Applications of PEGylation:

  • PEGylate carboxyl or amine surfaces
  • Add inert mass to proteins, immunogens, drug compounds and probes
  • Improve solubility (decrease aggregation) of proteins or peptides without affecting function
  • Protect proteins from proteolysis

Methods of Covalent Attachment:

  • Derivatize or crosslink carboxyls or primary amines using EDC and Sulfo-NHS
  • Crosslink amino terminus to primary amines using DSS, BS(PEG)n or other NHS-ester reagent
  • Crosslink amino terminus to sulfhydryl groups using Sulfo-SMCC or SM(PEG)n reagent

Why PEGylate a protein or peptide?

PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length Polyethylene Glycol Compounds

These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.

References:

  1. Hermanson, G.T. (2008). Bioconjugate Techniques, Academic Press. (Part No. 20036)
  2. Harris, J. M. and Zalipsky, S. Eds (1997). Poly(ethylene glycol), Chemistry and Biological Applications, ACS Symposium Series, 680.
  3. Harris, J. M. and Kozlowski, A. (2001). Improvements in protein PEGylation: pegylated interferons for treatment of hepatitis C. J. Control Release 72, 217-224.
  4. Veronese, F. and Harris, J.M. Eds. (2002). Peptide and protein PEGylation. Advanced Drug Delivery Review 54(4), 453-609.

Related Products:

Protein PEGylation technical guide (and PEG reagent selection guide)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
26120 CA(PEG)4
Formulation: 4-unit ethyleneglycol functionalized with carboxyl and amine ends; solid
Sufficient For: 15mL of typical 1X (25mM) reaction solution
100mg Product Instructions for product #26120 CA(PEG)4 MSDS for product #26120 CA(PEG)4 Certificate of Analysis for product #26120 CA(PEG)4 $143.00

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26121 CA(PEG)4
Formulation: 4-unit ethyleneglycol functionalized with carboxyl and amine ends; solid
Sufficient For: 151mL of typical 1X (25mM) reaction solution
1g Product Instructions for product #26121 CA(PEG)4 MSDS for product #26121 CA(PEG)4 Certificate of Analysis for product #26121 CA(PEG)4 $579.00

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26122 CA(PEG)8
Formulation: 8-unit ethyleneglycol functionalized with carboxyl and amine ends; solid
Sufficient For: 9mL of typical 1X (25mM) reaction solution
100mg Product Instructions for product #26122 CA(PEG)8 MSDS for product #26122 CA(PEG)8 Certificate of Analysis for product #26122 CA(PEG)8 $230.00

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26123 CA(PEG)8
Formulation: 8-unit ethyleneglycol functionalized with carboxyl and amine ends; solid
Sufficient For: 91mL of typical 1X (25mM) reaction solution
1g Product Instructions for product #26123 CA(PEG)8 MSDS for product #26123 CA(PEG)8 Certificate of Analysis for product #26123 CA(PEG)8 $999.00

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26124 CA(PEG)12
Formulation: 12-unit ethyleneglycol functionalized with carboxyl and amine ends; solid
Sufficient For: 6mL of typical 1X (25mM) reaction solution
100mg Product Instructions for product #26124 CA(PEG)12 MSDS for product #26124 CA(PEG)12 Certificate of Analysis for product #26124 CA(PEG)12 $254.00

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26125 CA(PEG)12
Formulation: 12-unit ethyleneglycol functionalized with carboxyl and amine ends; solid
Sufficient For: 65mL of typical 1X (25mM) reaction solution
1g Product Instructions for product #26125 CA(PEG)12 MSDS for product #26125 CA(PEG)12 Certificate of Analysis for product #26125 CA(PEG)12 $1,099.00

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26126 CA(PEG)24
Formulation: 24-unit ethyleneglycol functionalized with carboxyl and amine ends; waxy solid
Sufficient For: 3mL of typical 1X (25mM) reaction solution
100mg Product Instructions for product #26126 CA(PEG)24 MSDS for product #26126 CA(PEG)24 Certificate of Analysis for product #26126 CA(PEG)24 $316.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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