Thermo Scientific Pierce BS(PEG)n Crosslinkers include BS(PEG)5 and BS(PEG)9, which are bis-succinimide ester-activated PEG compounds for crosslinking between primary amines in proteins and other molecules.
BS(PEG)5 and BS(PEG)9 are bis-succinimide ester-activated PEG compounds for crosslinking between primary amines (–NH2) in proteins and other molecules. The N-hydroxysuccinimide ester (NHS) groups at either end of the PEG5 or PEG9 spacer react specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds.
- Reactive groups: sulfo-NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- NHS esters react with –NH2 groups at pH 7-9, forming stable amide bonds
- Water-soluble; compare to non-pegylated BS3 (also soluble) and DSS (water-insoluble)
- Membrane-impermeable, allowing for cell surface labeling
- Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
- Polyethylene glycol spacer arms help maintain conjugate solubility
- Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
- PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
- Ideal for small molecule or peptide conjugations
|Chemical structures of BS(PEG)5 and BS(PEG)12 crosslinking reagents.
Alternative Names for BS(PEG)n:
- dPEG NHS ester
- Bis-succinimide ester PEG 400
Crosslinker Application Guide -- search for recent literature references for this product
Bioconjugate Techniques, Academic Press (Part No. 20036)
Overview of crosslinking
Chemistry of crosslinking
Crosslinkers at a glance (selection guide)
Non-pegylated NHS Crosslinkers
Sulfhydryl-reactive PEG Crosslinkers
Other kinds of PEGylation Reagents