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BS(PEG)n PEG Crosslinkers - Amine Reactive


Amine-to-amine crosslinkers with polyethylene glycol (PEG) spacer arms.

Pierce BS(PEG)5 and BS(PEG)9 crosslinkers

Thermo Scientific Pierce BS(PEG)n Crosslinkers include BS(PEG)5 and BS(PEG)9, which are bis-succinimide ester-activated PEG compounds for crosslinking between primary amines in proteins and other molecules.

BS(PEG)5 and BS(PEG)9 are bis-succinimide ester-activated PEG compounds for crosslinking between primary amines (–NH2) in proteins and other molecules. The N-hydroxysuccinimide ester (NHS) groups at either end of the PEG5 or PEG9 spacer react specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds.

Highlights:

  • Reactive groups: sulfo-NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • NHS esters react with –NH2 groups at pH 7-9, forming stable amide bonds
  • Water-soluble; compare to non-pegylated BS3 (also soluble) and DSS (water-insoluble)
  • Membrane-impermeable, allowing for cell surface labeling
  • Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
  • Polyethylene glycol spacer arms help maintain conjugate solubility
  • Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
  • PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
  • Ideal for small molecule or peptide conjugations

Product Details:

Bis(succinimidyl) penta(ethylene glycol); BS(PEG)5
Bis(succinimidyl) nona(ethylene glycol); BS(PEG)9
 
Chemical structures of BS(PEG)5 and BS(PEG)12 crosslinking reagents.

Alternative Names for BS(PEG)n:

  • Bis-NHS-dPEG
  • NHS-PEG-NHS
  • BS(PEO)5
  • Bis(NHS)PEO5
  • BS-PEO9
  • dPEG NHS ester
  • Bis-succinimide ester PEG 400

Product References:

Crosslinker Application Guide -- search for recent literature references for this product
Bioconjugate Techniques, Academic Press (Part No. 20036)

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
Non-pegylated NHS Crosslinkers
Sulfhydryl-reactive PEG Crosslinkers
Other kinds of PEGylation Reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21581 BS(PEG)5
Formulation: Bis(succinimidyl) penta(ethylene glycol); amber oil liquid
Sufficient For: 188mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use
100mg Product Instructions for product #21581 BS(PEG)5 MSDS for product #21581 BS(PEG)5 Certificate of Analysis for product #21581 BS(PEG)5 $240.00

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21582 BS(PEG)9
Formulation: Bis(succinimidyl) nona(ethylene glycol); amber oil liquid
Sufficient For: 141mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use
100mg Product Instructions for product #21582 BS(PEG)9 MSDS for product #21582 BS(PEG)9 Certificate of Analysis for product #21582 BS(PEG)9 $318.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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