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BS3 (Bis[sulfosuccinimidyl] suberate)


A noncleavable, membrane-impermeable, water-soluble crosslinking agent for amines.

Pierce BS3 (Sulfo-DSS)

Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.

Because it contains the hydrophilic sulfonyl moiety, BS3 crosslinker is soluble up to ~100mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure. DSS, the non-water soluble analog of BS3 is also available for applications that require a less hydrophilic crosslinker (e.g., to effect intracellular crosslinking). DSS and BS3 have essentially identical crosslinking activity toward primary amines.

Highlights:

  • Reactive groups: sulfo-NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Water-soluble; compare with DSS
  • Membrane-impermeable, allowing for cell surface labeling
  • High-purity, crystalline reagent can be used to create high-purity crosslinked conjugates

Applications:

  • Crosslink cell-surface proteins prior to cell lysis and immunoprecipitation
  • Identify receptor-ligand interactions by chemical crosslinking
  • “Fix” protein interactions to allow identification of weak or transient protein interactions
  • Identify near-neighbor protein interactions
  • Protein crosslinking to create bioconjugates via single-step reactions
  • Immobilize proteins onto amine-coated surfaces

Product Details:

Bis(sulfosuccinimidyl) suberate; (BS3)
 
Chemical structure of BS3 crosslinking reagent.

Properties of BS3.
Alternative names Sulfo-DSS
Molecular formula C16H18N2O14S2Na2
Molecular weight 572.43
Spacer arm length 11.4 Å (8 atoms)
CAS number 82436-77-9
Storage conditions 4°C, protect from moisture, use only fresh solutions
Reactive groups NHS esters, react with primary amines at pH 7.0-9.0

BS3 Specifications:

We manufacture BS3 to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of BS3 is tested to meet the following minimum specifications.

  • Purity: > 93% by quantitative NMR (the highest standard for crosslinker purity)
  • Solubility: > 5.8 mg/mL in DI water, clear solution with no insoluble material

References:

  1. Dihazi, G.H. and Sinz, A. (2003). Rapid Commun. Mass Spectrom. 17, 2005-2014.
  2. Koller, D., et al. (2004). J. Biol. Chem. 279, 20387-20391.
  3. Law, B.K., et al. (2002). Mol. Cell. Biol. 22, 8184-8198.
  4. Mattson, G., et al. (1993). A practical approach to crosslinking. Molecular Biology Reports 17, 167-183.
  5. Muller, D.R., et al. (2001). Anal. Chem. 73, 1927-1934.
  6. Partis, M.D., et al. (1983). Crosslinking of proteins by omega-maleimido alkanoyl
    N-hydroxysuccinimide esters. J. Protein. Chem. 2, 263-277.
  7. Sinz, A. (2003). J. Mass Spectrom. 38, 1225-1237.
  8. Schutz, D.M., et al. (2004). Biochemistry 43, 4703-4715.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
BS3 mass tag pairs
DSS (membrane-permeable equivalent)
DTSSP (cleavable equivalent)
Bioconjugate Techniques (book)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21580 BS3
Formulation: Bis(sulfosuccinimidyl) suberate; powder solid
Sufficient For: 87mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #21580 BS3 MSDS for product #21580 BS3 Certificate of Analysis for product #21580 BS3 $100.00

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21585 BS3, No-Weigh Format
Formulation: Bis(sulfosuccinimidyl) suberate; solid
Sufficient For: 28mL of typical 1X (1mM) reaction solution (total), or 8 separate uses
8 x 2mg Product Instructions for product #21585 BS3, No-Weigh Format MSDS for product #21585 BS3, No-Weigh Format Certificate of Analysis for product #21585 BS3, No-Weigh Format $127.00

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21586 BS3
Formulation: Bis(sulfosuccinimidyl) suberate; powder solid
Sufficient For: 1747mL of typical 1X (1mM) reaction solution, or approx. 500 uses at 2mg reagent per use
1g Product Instructions for product #21586 BS3 MSDS for product #21586 BS3 Certificate of Analysis for product #21586 BS3 $1,150.00

Add to cart
21579B BS3
Formulation: Bis(sulfosuccinimidyl) suberate; powder solid
Custom MSDS for product #21579B BS3 Certificate of Analysis for product #21579B BS3

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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