Reagent kit for sandwich ELISA of bovine interleukin-6 (IL-6).
Assay IL-6 (bovine interleukin-6) levels in bovine cell culture supernatant samples with our Thermo Scientific Bovine IL-6 ELISA Kits.
This bovine IL-6 ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant bovine interleukin-6 (IL-6)
Format: colorimetric ELISA (TMB substrate) for 96-well microplates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 78 to 5000pg/mL
Sample Types: cell culture supernatant
Total Assay Time: 3.5 hours
Sample Size: 100μL
Specificity: no significant cross-reaction with recombinant bovine IL-1β, IL-2, IL-4, IL-8, IFNγ, or TNFα.
Video of the Thermo Scientific IL-6 ELISA Kit
The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
Bovine IL-6 ELISA standard curve. An example standard curve for the Thermo Scientific Bovine IL-6 ELISA Reagent Kit (Part No. ESS0029), with error bars based on six replicates for each interleukin-6 standard concentration.