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Bovine IL-1 beta ELISA Kits

Reagent kit for sandwich ELISA of bovine interleukin-1 beta (IL-1b).

Thermo Scientific Bovine IL-1 beta ELISA Kits

Assay IL-1β (bovine interleukin-1 beta) levels in bovine cell culture supernatant samples with our Thermo Scientific Bovine IL-1 beta ELISA Kits.

This bovine IL-1 beta ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.


  • Target: natural and recombinant bovine interleukin-1 beta (IL-1β)
  • Format: colorimetric ELISA (TMB substrate) for 96-well microplates
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31 to 2000pg/mL
  • Sensitivity: undetermined
  • Sample Types: cell culture supernatant
  • Total Assay Time: 3.5 hours
  • Sample Size: 100μL
  • Specificity: no significant cross-reaction with recombinant bovine IL-2, IL-4, IL-6, IL-8, IFNγ, or TNFα.
Video of the Thermo Scientific IL-1 beta ELISA Kit


The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.


Cow IL-1 beta ELISA standard curve
Bovine IL-1 beta ELISA standard curve. An example standard curve for the Thermo Scientific Bovine IL-1 beta ELISA Reagent Kit (Part No. ESS0027), with error bars based on six replicates for each interleukin-1 beta standard concentration.

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This product is for In Vitro experimental use only. Not for resale without express authorization.

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
ESS0027 Bovine IL-1 beta ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
IL-1 beta Coating Antibody, 0.625mL
IL-1 beta Detection Antibody, 0.625mL
Bovine IL-1 beta Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0027 Bovine IL-1 beta ELISA Reagent Kit MSDS for product #ESS0027 Bovine IL-1 beta ELISA Reagent Kit $425.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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