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Bond-Breaker TCEP Solution, Neutral pH


Stable, odorless, 10X solution for efficient sample reduction before SDS-PAGE.

Bond-Breaker TCEP Solution, Neutral pH

Thermo Scientific Bond-Breaker TCEP Solution, Neutral pH is a stable, 0.5M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulfide bonds.

Bond-Breaker TCEP Solution is a potent, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. This product is an effective and convenient replacement for B-mercaptoethanol or DTT in SDS-PAGE sample buffers. The neutral pH of this reagent provides sharp bands and avoids exposing proteins to the strong acid of TCEP•HCl, which can result in hydrolysis and carbohydrate modification.

Highlights:

  • Odorless – unlike DTT and β-ME, TCEP is odor-free, contributing to a healthier lab environment
  • Efficient – 5 to 50mM TCEP thoroughly reduces most peptide or protein disulfide bonds within a few minutes (i.e., just as effective as DTT)
  • Specific – selective and complete reduction of even the most stable water-soluble alkyl disulfides
  • Fast – reduces protein disulfides at room temperature and pH 5 in less than five minutes
  • Stable – resistant to air oxidation; nonvolatile and nonreactive toward other functional groups found in proteins
  • Versatile – reduces peptides and proteins over a broad range of pH, salt, detergent and temperature conditions
  • Compatible – removal of the reducing agent is not necessary before most applications, (e.g. histidine-tagged protein purification, maleimide conjugations), because TCEP does not contain sulfhydryl groups

Product Details:

Structure of TCEP-HCl (Tris[2-carboxyethyl]phosphine hydrochloride)

Protocol for using Bond-Breaker TCEP to reduce proteins for SDS-PAGE analysis.
Protocol for using Thermo Scientific Bond-Breaker TCEP Solution to reduce proteins for SDS-PAGE analysis.

 

Properties of Thermo Scientific Bond-Breaker TCEP Solution. See TCEP-HCl (Part No. 20490) for chemical properties of tris(2-carboxyethyl)phosphine.
Active ingredient tris(2-carboxyethyl)phosphine (TCEP)
TCEP concentration 500-550mM
pH 6.6 ± 0.1
Storage conditions room temperature

Considerations for use of Bond-Breaker TCEP Solution:

  • Reduction occurs over a wide range of pH (pH 4.0-9.0) and temperature (5 to 95°C).
  • Most proteins are reduced efficiently without a denaturant. However, adding a denaturant such as guanidine•HCl may aid in exposing internal disulfides to the Immobilized TCEP.
  • Urea is not recommended as a denaturant as it forms cyanates that react with sulfhydryl groups.
  • Do not allow metals to contact the TCEP solution as this will decrease TCEP activity.
  • Including 5 to 20mM EDTA in the sample buffer during reduction helps prevent reoxidation of the sulfhydryl groups by divalent metals such as Zn 2+, Cu 2+ and Mg 2+.
  • The reduced sample should be used immediately after reduction because disulfides will reform over time.

References

  1. Irsch, T. and Krauth-Siegel, R.L. (2004) Glyoxalase II of African trypanosomes is trypanothione-dependent. J. Biol. Chem.279, 22209-17.
  2. Schmidt, H. and Krauth-Siegel, R.L. (2003) Functional and physicochemical characterization of the thioredoxin system in Trypanosoma brucei. J. Biol. Chem.278, 46329-36.

Related Resources:

Overview of crosslinking and protein modification
Overview of protein electrophoresis

Related Products:

TCEP•HCl
Immobilized TCEP Disulfide Reducing Gel
All Disulfide Reducing Agents
Electrophoresis Sample Loading Buffers


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
77720 Bond-Breaker TCEP Solution, Neutral pH
Formulation: 500mM Tris(2-carboxyethyl)phosphine in aqueous solution at neutral pH
Sufficient For: Preparing 50mL of 50mM TCEP solution
5mL Product Instructions for product #77720 Bond-Breaker TCEP Solution, Neutral pH MSDS for product #77720 Bond-Breaker TCEP Solution, Neutral pH Certificate of Analysis for product #77720 Bond-Breaker TCEP Solution, Neutral pH $120.00

Add to cart
77720B Bond-Breaker TCEP Solution, Neutral pH
Formulation: 500mM Tris(2-carboxyethyl)phosphine in aqueous solution at neutral pH
Custom MSDS for product #77720B Bond-Breaker TCEP Solution, Neutral pH Certificate of Analysis for product #77720B Bond-Breaker TCEP Solution, Neutral pH

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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