Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 angstroms).
- Reactive groups: NHS ester and maleimide
- Reactive towards: amino and sulfhydryl groups
- C3-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
- NHS ester end couples with primary amines at pH 7-9, forming stable amide bonds
- Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
- Water-insoluble (dissolve first in DMF or DMSO)
- Short aliphatic spacer has low potential for eliciting an immune response
|Chemical structure of BMPS crosslinking reagent.
- McKenzie, J.A., Raison, R.L. and Rivett, E.E. (1988). Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins. J. Protein Chem. 7(5), 581-592.
- Kitagawa, T., et al. (1981). Chem. Pharm. Bull. 29(4), 1130-1135.
- Kitagawa, T. (1981). "Enzyme labeling with N-hydroxysuccinimidyl ester of maleimide", In Ishikawa, E., Kawai, T. and Miyai, K. (Eds.), Enzyme Immuno-assay (pp. 81-89). IGAKU-SHOIN, Tokyo-New York.
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