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A short crosslinker for conjugating glycoprotein carbohydrates to sulfhydryl groups.

Pierce BMPH

Thermo Scientific Pierce BMPH is a short, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).


  • Reactive groups: maleimide and hydrazide
  • Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
  • Short (8.1A), sulfhydryl-to-aldehyde crosslinker with simple spacer arm (noncleavable)
  • Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
  • Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
  • Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
  • Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

Product Details:

N-beta-Maleimidopropionic acid hydrazide-TFA; BMPH
Chemical structure of BMPH crosslinking reagent.


  1. Chrisey, L.A., Lee, G.U. and O’Ferrall, C.E. (1996). Covalent attachment of synthetic DNA to self-assembled monolayer films. Nucleic Acids Research 24(15), 3031-3039.
  2. Kitagawa, T., et al. (1980). Preparation and characterization of hetero-bifunctional crosslinking reagents for protein modifications. Chem. Pharm. Bull. 29(4), 1130-1135.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking
Protein glycosylation technical guide

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22297 BMPH
Formulation: N-beta-Maleimidopropionic acid hydrazide-TFA; solid
Sufficient For: 168mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22297 BMPH MSDS for product #22297 BMPH Certificate of Analysis for product #22297 BMPH $115.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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