Thermo Scientific Pierce BMPH is a short, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
- Reactive groups: maleimide and hydrazide
- Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
- Short (8.1A), sulfhydryl-to-aldehyde crosslinker with simple spacer arm (noncleavable)
- Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
- Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
- Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
- Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups
|Chemical structure of BMPH crosslinking reagent.
- Chrisey, L.A., Lee, G.U. and O’Ferrall, C.E. (1996). Covalent attachment of synthetic DNA to self-assembled monolayer films. Nucleic Acids Research 24(15), 3031-3039.
- Kitagawa, T., et al. (1980). Preparation and characterization of hetero-bifunctional crosslinking reagents for protein modifications. Chem. Pharm. Bull. 29(4), 1130-1135.
Crosslinker Application Guide -- search for recent literature references for this product
Overview of crosslinking
Chemistry of crosslinking
Protein glycosylation technical guide
Crosslinkers at a glance (selection guide)