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BMOE


A short-arm, maleimide crosslinker for sulfhydryl conjugation.

Pierce BMOE

Thermo Scientific Pierce BMOE is a short-arm, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).

Highlights:

  • Reactive groups: maleimide (both ends)
  • Reactive towards: sulfhydryl groups
  • Short (8.0A), sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 7-atom spacer arm
  • Shortest bismaleimide crosslinker available for close proximity crosslinking
  • Water-insoluble – dissolve first in DMF or DMSO, then add to aqueous reaction buffers
  • Noncleavable

Product Details:

Bismaleimidoethane; BMOE
 
Chemical structure of BMOE crosslinking reagent.

References:

  1. Chen, L.L., et al. (1991). Production of multimeric forms of CD4 through a sugar-based crosslinking strategy. J. Biol. Chem. 266(27), 18237-18243.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
22323 BMOE
Formulation: Bismaleimidoethane; solid
Sufficient For: 1136mL of typical 1X (0.2mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22323 BMOE MSDS for product #22323 BMOE Certificate of Analysis for product #22323 BMOE $104.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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