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A mid-length, maleimide crosslinker for sulfhydryl conjugation.

Pierce BMB

Thermo Scientific Pierce BMB is a mid-length, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).


  • Reactive groups: maleimide (both ends)
  • Reactive towards: sulfhydryl groups
  • Mid-length (10.9A), sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 9-atom spacer arm
  • Water-insoluble – dissolve first in DMF or DMSO, then add to aqueous reaction buffers
  • Noncleavable

Product Details:

1,4-Bismaleimidobutane; BMB
Chemical structure of BMB crosslinking reagent.


  1. Chen, L.L., et al. (1991). J. Biol. Chem. 266(27), 18237-18243.
  2. Stalteri, M.A. and Mather, S.J. (1995). Bioconjug. Chem. 6, 179-186.
  3. Yi, F., et al. (1991). J. Biol. Chem. 266(6), 3900-3906.
  4. O’Sullivan, M.J., et al. (1979). Anal. Biochem. 100, 100-108.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22331 BMB
Formulation: 1,4-Bismaleimidobutane; solid
Sufficient For: 1007mL of typical 1X (0.2mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22331 BMB MSDS for product #22331 BMB Certificate of Analysis for product #22331 BMB $106.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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