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Blocker Casein Blocking Buffers


Hammarsten Grade casein for immunoassay blocking steps.

Blocker Casein Blocking Buffer

Thermo Scientific Blocker Casein Blocking Buffers are ready-to-use, PBS or TBS solutions of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

These blocking buffers contains casein protein that is purified from milk by the Hammarsten method. The phosphate- and Tris-buffered saline formulations are used for blocking excess binding sites on membranes and microplates in antibody-based detection methods. Blocker Casein Buffers are 1% (w/v) casein, which corresponds to the optimal concentration for most applications.

Highlights:

  • Purified casein – single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
  • Easy to use – 1% casein solutions are ready to use; can be diluted further as needed
  • Flexible – available in PBS and TBS formulations to suit a variety of applications
  • Safe – stable, thimerosal-free formulations

References:

  1. Alegria-Schaffer, A., et al. (2009). Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 463:573-99.
  2. Nemzek, J.A. et al. (2000). Am. J. Physiol. Lung Cell. Mol. Physiol. 278:L512-20.
  3. Stuyver, L.J., et al. (2003). Antimicrob. Agents Chemother. 47:244-254.
  4. Wolfman, J.C., et al. (2002). Mol. Cell. Biol. 22:1589-1606.

Related Resources:

Blocking buffers for Western blot and ELISA -- technical guide
Blocking strategies for immunohistochemistry -- technical guide

Related Products:

All Blocking Buffers


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
37582 Blocker Casein in PBS
Formulation: 1% (w/v) casein (Hammarsten grade) in 100mM sodium phosphate, 150mM NaCl, pH 7.4 containing Kathon Anti-microbial Agent
Sufficient For: 3 blots or microplates at 30mL each
100mL Product Instructions for product #37582 Blocker Casein in PBS MSDS for product #37582 Blocker Casein in PBS Certificate of Analysis for product #37582 Blocker Casein in PBS $31.00

Add to cart
37528 Blocker Casein in PBS
Formulation: 1% (w/v) casein in phosphate-buffered saline, pH 7.4, with Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37528 Blocker Casein in PBS MSDS for product #37528 Blocker Casein in PBS Certificate of Analysis for product #37528 Blocker Casein in PBS $119.00

Add to cart
37528B Blocker Casein in PBS
Formulation: 1% (w/v) casein in phosphate-buffered saline, pH 7.4, with Kathon preservative
Custom MSDS for product #37528B Blocker Casein in PBS Certificate of Analysis for product #37528B Blocker Casein in PBS
37583 Blocker Casein in TBS
Formulation: 1% (w/v) casein (Hammarsten grade) in 25mM Tris, 150mM NaCl, pH 7.4 containing Kathon Anti-microbial Agent
Sufficient For: 3 blots or microplates at 30mL each
100mL Product Instructions for product #37583 Blocker Casein in TBS MSDS for product #37583 Blocker Casein in TBS Certificate of Analysis for product #37583 Blocker Casein in TBS $31.00

Add to cart
37532 Blocker Casein in TBS
Formulation: 1% (w/v) casein in Tris-buffered saline, pH 7.4, with Kathon preservative
Sufficient For: 30 blots or microplates at 30mL each
1L Product Instructions for product #37532 Blocker Casein in TBS MSDS for product #37532 Blocker Casein in TBS Certificate of Analysis for product #37532 Blocker Casein in TBS $119.00

Add to cart
37532B Blocker Casein in TBS
Formulation: 1% (w/v) casein in Tris-buffered saline, pH 7.4, with Kathon preservative
Custom MSDS for product #37532B Blocker Casein in TBS Certificate of Analysis for product #37532B Blocker Casein in TBS

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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