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Biotin Conjugated Proteins and Enzymes


For signal amplification and controls in avidin-biotin methods.

Bovine Serum Albumin, Biotinylated

Thermo Scientific Pierce Biotinylated Proteins include biotin-labeled proteins (BSA), enzymes (HRP, AP) and fluorophores (FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.

Biotinylated HRP and biotinylated AP are most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method. Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluor molecule is tagged with only one biotin molecule. However, the fluorescent variant of traditional ABC staining is possible with this unique molecule.

Highlights:

  • Biotinylated enzymes – biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining
  • Biotinylated FITC – amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC)
  • Biotinylated controls – use biotinylated bovine serum albumin (B-BSA) or any one of these molecules as positive controls in biotin quantitation methods

Product Details:

Biotin, also known as vitamin H, is a small molecule (MW 244.3) that is present in tiny amounts in all living cells and is critical for a number of biological processes. The valeric acid side chain of the biotin molecule can be derivatized in order to incorporate various reactive groups that are used to attach biotin to other molecules. In the context of immunohistochemistry (IHC), biotin is conjugated to antibodies or to the enzyme reporters used to detect target antigens.

Diagram of immunodetection using Avidin-Biotin Complex (ABC) staining. Legend for Avidin-Biotin Complex diagram

Diagram of the Avidin-Biotin Complex (ABC) method of signal amplification for immunohistochemical (IHC) staining.

Related Resources:

Avidin-biotin complex method for IHC technical guide
Protein biotinylation technical guide

Related Products:

ABC Staining Kits
Biotinylation Reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
29129 Biotin
Formulation: Vitamin H; Hexahydro-2-oxo-1H-thieno (3,4-d)imidazole-4-pentanoic acid); Salt-free powder
1g MSDS for product #29129 Biotin Certificate of Analysis for product #29129 Biotin $59.00

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29130 Bovine Serum Albumin, Biotinylated
Formulation: Lyophilized powder from protein in buffered solution
25mg MSDS for product #29130 Bovine Serum Albumin, Biotinylated Certificate of Analysis for product #29130 Bovine Serum Albumin, Biotinylated $146.00

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29130B Bovine Serum Albumin, Biotinylated
Formulation: Lyophilized powder from protein in buffered solution
Custom MSDS for product #29130B Bovine Serum Albumin, Biotinylated Certificate of Analysis for product #29130B Bovine Serum Albumin, Biotinylated
29139 Horseradish Peroxidase, Biotinylated
Formulation: Lyophilized powder from protein in buffered solution
5mg MSDS for product #29139 Horseradish Peroxidase, Biotinylated Certificate of Analysis for product #29139 Horseradish Peroxidase, Biotinylated $136.00

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29339 Alkaline Phosphatase, Biotinylated
Formulation: Lyophilized powder from protein in buffered saline solution containing bovine serum albumin
1mg MSDS for product #29339 Alkaline Phosphatase, Biotinylated Certificate of Analysis for product #29339 Alkaline Phosphatase, Biotinylated $215.00

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22030 Biotin-Fluorescein Conjugate
Formulation: Salt-free powder
5mg Product Instructions for product #22030 Biotin-Fluorescein Conjugate MSDS for product #22030 Biotin-Fluorescein Conjugate Certificate of Analysis for product #22030 Biotin-Fluorescein Conjugate $299.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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