Bioconjugate Techniques (2nd edition, 2008) is a book that is widely recognized as the best single source of information about the chemistry and research methods of biomolecular crosslinking, surface attachment and labeling.
This is a must-have resource when searching for methods that will facilitate the conjugation of molecules to new reagents or for novel applications. As the field of bioconjugation continues to grow, researchers in academia and industry look to link two or more molecules to form novel complexes with uniquely combined properties of the individual components. Greg Hermanson, a recognized bioconjugation technology expert, provides a complete update and major expansion of his original 2002 work which is recognized as the definitive reference in the field.
This 1202-page book is an extremely valuable source of information with easy-to-understand strategies, 850 figures and illustrations and up-to-date references. It comprehensively covers every reagent system for bioconjugation in research, diagnostics, and therapeutic development. It also discusses the latest advances in bioconjugation methods applied to nanotechnology and protein-protein interactions.
Updated Sections and Chapters:
- Bioconjugate Chemistry
- Functional Targets; The Chemistry of Reactive Groups
- Bioconjugate Reagents
- Zero-Length Crosslinkers; Homobifunctional Crosslinkers; Heterobifunctional Crosslinkers; Trifunctional Crosslinkers; Cleavable Reagent Systems; Fluorescent Probes; Bifunctional Chelating Agents and Radioimmunoconjugates; Biotinylation Reagents; Iodination Reagents
- Bioconjugate Applications
- Preparation of Hapten-Carrier Immunogen Conjugates; Antibody Modification and Conjugation; Immunotoxin Conjugation Techniques; Preparation of Liposome Conjugates and Derivatives; Avidin - Biotin Systems; Preparation of Colloidal-Gold-Labeled Proteins; Modification with Synthetic Polymers; Enzyme Modification and Conjugation; Nucleic Acid and Oligonucleotide Modification and Conjugation
Entirely New Chapters and Topics:
- Dendrimers and dendrons; Silane Coupling Agents; Microparticles and Nanoparticles; Buckyballs, Fullerenes, and Carbon Nanotubes; Mass Tags and Isotope Tags; Chemoselective Ligation: Bioorthogonal Reagents; Discrete PEG Compounds; Bioconjugation in the Study of Protein Interactions
- Oxidation of Amino Acids in Proteins and Peptides; Solvent Accessibility of Functional Targets in Proteins; Carbohydrate and Glycan Conjugation Methods; Introduction of Saccharide or Glycan Groups; Cycloaddition Reactions; Cyanine Dye Derivatives; Lanthanide Chelates for Time-Resolved Fluorescence; Quantum Dot Nanocrystals; Glycan Biotinylation Reagents
Review of Crosslinking Methods
Chemistry of Crosslinking
Protein and Antibody Labeling Reagents
The 2nd edition (Part No. 20036) replaces the 1st edition (obsolete Part No. 20002).