Bioconjugate Techniques, 3rd Edition (2013) by Greg T. Hermanson is a major update to a book that is widely recognized as the definitive reference guide to the field of bioconjugation.
Bioconjugate Techniques is a complete textbook and protocols-manual for life scientists wishing to learn and master the biomolecular crosslinking, labeling and immobilization techniques that form the basis of many laboratory applications. The book is also an exhaustive and robust reference for researchers looking to develop novel conjugation strategies for entirely new applications. It also contains an extensive introduction to the field of bioconjugation that covers all of the major applications of the technology used in diverse scientific disciplines as well as containing tips for designing the optimal bioconjugate for any purpose.
- A single, definitive source for all methods of labeling or conjugation of biomolecules
- Diagrams of chemical structures for hundreds of reagents and reaction schemes
- Hundreds of step-by-step protocols for making conjugates, plus many tips for optimizing each reaction, extensively referenced to the literature
- Complete treatment of all classes of biomolecules: proteins, carbohydrates, nucleic acids
- New full-color illustrations and reactions
- New, extensive general introduction to the field of bioconjugation
- Entirely new chapter on the methods used for coupling affinity ligands to solid supports, including beads, membranes, and surfaces
The third edition (ISBN-10: 0123822394; ISBN-13: 9780123822390) provides completely updated material on previous chapters and an entirely new organization of subject matter that logically presents this vast field in a clear and understandable way. It continues to offer the best single source on the reactions, methods, and applications of bioconjugation for novices and experts alike. The new edition is hardbound in a larger format (approx. 9 x 11") with hundreds of new full color figures and illustrations throughout.
1st Edition (1996)
Original Part No. 20002.
2nd Edition (2008)
Original Part No. 20036.
The book also contains a new chapter on the reactions and methods that can be used to immobilize affinity ligands on chromatography supports and other solid-phase surfaces. It is the most complete overview of this application area ever presented, containing the latest reactions and protocols for support activation and ligand coupling that can be used with particles, membranes, and surfaces of every type. This additional chapter finally provides a satisfying replacement (and greatly updated treatment) for the out-of-print Immobilized Affinity Ligand Techniques (1992) by Hermanson, Mallia and Smith.
Hermanson, GT, Mallia, AK and Smith, PK. (1992). Immobilized Affinity Ligand Techniques. Academic Press, Inc.
The essential contents of this out-of-print book are updated and contained in the third edition of Bioconjugate Techniques.
Praise for the Second Edition:
"[T]his is the best book available that describes bioconjugation reactions, in terms of both its comprehensive coverage and its chemical accuracy. It is a ‘must have’ for any lab that routinely prepares modified proteins or oligonucleotides, and it can serve as a great starting point for learning about the many new components that have been used to study or enhance biomolecular function." —Matthew B. Francis (2008) Updating the bioconjugation catalog. Nature Chemical Biology 4, 717.
Review of Crosslinking Methods
Chemistry of Crosslinking
Protein and Antibody Labeling Reagents