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Antibody Binding and Elution Buffers


Ensure optimal binding and elutions conditions for almost any affinity application.

Antibody binding and elution buffers

Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Highlights:

  • Ready to use – carefully formulated and prefiltered 1X solutions
  • Three binding buffers – optimized for Protein A, Protein G or Protein A/G
  • Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)
  • Versatile – elution buffers are appropriate for antibody-antigen affinity purification

Item-Specific Features:

  • Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
  • Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
  • Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
  • IgG Elution Buffer: pH 2.8; amine-based
  • Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
  • Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

Related Resources:

Review of antibody purification methods

Related Products:

Antibody Purification Resins and Kits
Antigen Immobilization Resins and Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21001 Protein A IgG Binding Buffer
Formulation: Proprietary buffer (pH 8) with EDTA and glycerol
Sufficient For: Maximum binding of IgG to Protein A resin
1L Product Instructions for product #21001 Protein A IgG Binding Buffer MSDS for product #21001 Protein A IgG Binding Buffer Certificate of Analysis for product #21001 Protein A IgG Binding Buffer $78.00

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21007 Protein A IgG Binding Buffer
Formulation: Proprietary buffer (pH 8) with EDTA and glycerol
Sufficient For: Maximum binding of IgG to Protein A resin
3.75L Product Instructions for product #21007 Protein A IgG Binding Buffer MSDS for product #21007 Protein A IgG Binding Buffer Certificate of Analysis for product #21007 Protein A IgG Binding Buffer $219.00

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21019 Protein G IgG Binding Buffer
Formulation: Proprietary buffer (pH 5) with sodium azide
Sufficient For: Maximum binding of IgG to Protein G resin
1L Product Instructions for product #21019 Protein G IgG Binding Buffer MSDS for product #21019 Protein G IgG Binding Buffer Certificate of Analysis for product #21019 Protein G IgG Binding Buffer $71.00

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21011 Protein G IgG Binding Buffer
Formulation: Proprietary buffer (pH 5) with sodium azide
Sufficient For: Maximum binding of IgG to Protein G resin
3.75L Product Instructions for product #21011 Protein G IgG Binding Buffer MSDS for product #21011 Protein G IgG Binding Buffer Certificate of Analysis for product #21011 Protein G IgG Binding Buffer $215.00

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54200 Protein A/G IgG Binding Buffer
Formulation: Proprietary buffer (pH 8) with EDTA
Sufficient For: Maximum binding of IgG to Protein A/G resin
240mL Product Instructions for product #54200 Protein A/G IgG Binding Buffer MSDS for product #54200 Protein A/G IgG Binding Buffer Certificate of Analysis for product #54200 Protein A/G IgG Binding Buffer $40.00

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21004 IgG Elution Buffer
Formulation: Proprietary amine-buffer (pH 2.8)
Sufficient For: High-yield dissociation of IgG from affinity resins
1L Product Instructions for product #21004 IgG Elution Buffer MSDS for product #21004 IgG Elution Buffer Certificate of Analysis for product #21004 IgG Elution Buffer $73.00

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21009 IgG Elution Buffer
Formulation: Proprietary amine-buffer (pH 2.8)
Sufficient For: High-yield dissociation of IgG from affinity resins
3.75L Product Instructions for product #21009 IgG Elution Buffer MSDS for product #21009 IgG Elution Buffer Certificate of Analysis for product #21009 IgG Elution Buffer $182.00

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21028 IgG Elution Buffer, pH 2.0
Formulation: Proprietary amine-buffer (pH 2.0)
Sufficient For: high-yield dissociation of IgG from affinity resins
1L MSDS for product #21028 IgG Elution Buffer, pH 2.0 Certificate of Analysis for product #21028 IgG Elution Buffer, pH 2.0 $72.00

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21020 Gentle Ag/Ab Binding Buffer, pH 8.0
Formulation: Sodium borate buffer (pH 8)
Sufficient For: Phosphate-free binding of IgG for direct compatibility with Gentle Ag/Ab Elution Buffer
1L Product Instructions for product #21020 Gentle Ag/Ab Binding Buffer, pH 8.0 MSDS for product #21020 Gentle Ag/Ab Binding Buffer, pH 8.0 Certificate of Analysis for product #21020 Gentle Ag/Ab Binding Buffer, pH 8.0 $111.00

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21012 Gentle Ag/Ab Binding Buffer, pH 8.0
Formulation: Sodium borate buffer (pH 8)
Sufficient For: Phosphate-free binding of IgG for direct compatibility with Gentle Ag/Ab Elution Buffer
3.75L Product Instructions for product #21012 Gentle Ag/Ab Binding Buffer, pH 8.0 MSDS for product #21012 Gentle Ag/Ab Binding Buffer, pH 8.0 Certificate of Analysis for product #21012 Gentle Ag/Ab Binding Buffer, pH 8.0 $211.00

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21027 Gentle Ag/Ab Elution Buffer, pH 6.6
Formulation: Proprietary, high-molarity buffer (pH 6.55)
Sufficient For: Near-neutral elution of IgG
500mL Product Instructions for product #21027 Gentle Ag/Ab Elution Buffer, pH 6.6 MSDS for product #21027 Gentle Ag/Ab Elution Buffer, pH 6.6 Certificate of Analysis for product #21027 Gentle Ag/Ab Elution Buffer, pH 6.6 $99.00

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21013 Gentle Ag/Ab Elution Buffer, pH 6.6
Formulation: Proprietary, high-molarity buffer (pH 6.55)
Sufficient For: Near-neutral elution of IgG
3.75L Product Instructions for product #21013 Gentle Ag/Ab Elution Buffer, pH 6.6 MSDS for product #21013 Gentle Ag/Ab Elution Buffer, pH 6.6 Certificate of Analysis for product #21013 Gentle Ag/Ab Elution Buffer, pH 6.6 $297.00

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21013B Gentle Ag/Ab Elution Buffer, pH 6.6
Formulation: Proprietary, high-molarity buffer (pH 6.55)
Sufficient For: Near-neutral elution of IgG
Custom MSDS for product #21013B Gentle Ag/Ab Elution Buffer, pH 6.6
21030 Gentle Ag/Ab Binding and Elution Buffer Kit
Formulation: Multi-component kit
Sufficient For: Binding and near-neutral elution of antibodies with Protein A or G

Kit Contents:
Pierce Gentle Ag/Ab Binding Buffer, 100mL
Pierce Gentle Ag/Ab Elution Buffer, 100mL
200mL-kit Product Instructions for product #21030 Gentle Ag/Ab Binding and Elution Buffer Kit MSDS for product #21030 Gentle Ag/Ab Binding and Elution Buffer Kit Certificate of Analysis for product #21030 Gentle Ag/Ab Binding and Elution Buffer Kit $106.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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