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Mammalian beta-Galactosidase Assay Kit


One reagent formula for cell- or lysate-based assays for better signal in less time.

Mammalian beta-Galactosidase Assay Kit

The Thermo Scientific Mammalian β-Galactosidase Assay Kit provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Highlights:

  • Choose stand-alone assay reagent or complete kit for cell lysis and detection
  • Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
  • Adaptable to 96-well microplates or tube assay formats
  • Perform homogeneous screening assays or measure activity secondarily after cell lysis
  • Also compatiible with prepared lysates from other species such as bacteria

Product Details:

Performance comparison of beta-galactosidase assays. Performance comparison of beta-galactosidase assays. C6 cells expressing β-galactosidase were grown in 96-well plates. Cells were lysed and assayed using Thermo Scientific M-PER Mammalian Protein Extraction Reagent and Pierce β-Gal Assay Reagent or corresponding Supplier X reagents. Reactions were stopped with 150μL of Stop Solution provided in each kit, and absorbances were measured at 405nm using a plate-reading spectrophotometer.

 

Comparison of beta-galactosidase assay kit protocols. The Thermo Scientific Mammalian β-Galactosidase Assay Kit uses the all-in-one Pierce β-Gal Assay Reagent that streamlines the traditional procedures used by other commercially available kits.
Thermo Scientific Kit Supplier X Kit
  • Remove media from plate.
  • Add Pierce β-Gal Assay Reagent and incubate at 37°C for 30min.
  • Stop reaction (optional).
  • Measure absorbance at 405nm.
  • Remove media from plate.
  • Wash cells twice with PBS.
  • Dilute 5X Reporter Lysis Buffer to 1X.
  • Add Reporter Lysis Buffer; incubate 15min.
  • Scrape cells.
  • Remove cells to a clean tube, vortex and centrifuge for 2min at 4°C for 30min.
  • Add 2X Assay Buffer; incubate at 37°C for 30min.
  • Stop the reactions.
  • Measure absorbance ata 405nm.
Four (4) steps Nine (9) steps

Related Products:

Yeast β-Galactosidase Assay Kit
M-PER Mammalian Protein Extraction Reagent


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
75707 Pierce Mammalian beta-Galactosidase Assay Kit
Formulation: Multi-component kit
Sufficient For: 250 microplate wells of adherent cells

Kit Contents:
Pierce beta-Galactosidase Assay Reagent, 25mL
M-PER Mammalian Protein Extraction Reagent, 25mL
Stop Solution, 25mL
75mL kit Product Instructions for product #75707 Pierce Mammalian beta-Galactosidase Assay Kit MSDS for product #75707 Pierce Mammalian beta-Galactosidase Assay Kit Certificate of Analysis for product #75707 Pierce Mammalian beta-Galactosidase Assay Kit $194.00

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75705 Pierce beta-Galactosidase Assay Reagent
Formulation: Proprietary aqueous solution, pH 7.3
Sufficient For: 250 microplate wells of adherent cells
25mL Product Instructions for product #75705 Pierce beta-Galactosidase Assay Reagent MSDS for product #75705 Pierce beta-Galactosidase Assay Reagent Certificate of Analysis for product #75705 Pierce beta-Galactosidase Assay Reagent $91.00

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75710 Pierce beta-Galactosidase Assay Reagent
Formulation: Proprietary aqueous solution, pH 7.3
Sufficient For: 750 microplate wells of adherent cells
3 x 25mL Product Instructions for product #75710 Pierce beta-Galactosidase Assay Reagent Certificate of Analysis for product #75710 Pierce beta-Galactosidase Assay Reagent $192.00

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75706 Pierce beta-Galactosidase Assay Stop Solution
Formulation: Proprietary aqueous solution, pH ≥ 10.5
Sufficient For: 250 microplate wells
25mL MSDS for product #75706 Pierce beta-Galactosidase Assay Stop Solution $42.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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