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Pierce Avidin Agarose


Strong biotin interaction creates a nearly irreversible bond.

Pierce Avidin AgaroseThermo Scientific Pierce Avidin Agarose can be used in a variety of applications for the affinity purification of biotinylated macromolecules.

In one variation, an antibody that has an affinity for a particular antigen is labeled with biotin. Cells containing the antigen are lysed, then incubated with the biotinylated antibody to form a typical antigen/antibody complex. To isolate the antigen, the crude mixture is passed through an immobilized avidin or streptavidin column, which will bind the complex. After appropriate washes, the antigen can then be eluted from the column with a pH 2.8 elution buffer. The biotinylated antibody is retained by the column. 

Highlights:

  • Strong , nearly irreversible biotin:avidin interaction
  • Binding biotinylated anti-transferrinfrom serum (1)
  • Binding biotinyl peptides and elution with a SDS/Urea solution (2)
  • Hybridization of biotinylated RNA to its complementary DNA and binding to immobilized avidin, with subsequent elution of the single-stranded DNA (3)
  • Purification of double-stranded DNA (4)
  • Approximate Binding Capacity >20µg biotin/mL resin

References:

  1. Manning, J., Pellegrini, M. and Davidson, N. (1977). Biochemistry 16, 1364-1370.
  2. Pellegrini, M., Holmes, D.S. and Manning, J. (1977). Nucl. Acids Res. 4, 2961-2973.
  3. Swack, J.A., Zander, G.L. and Utter, M.F. (1978). Anal. Biochem. 87, 114-126.
  4. Wilchek, M. and Bayer, E.A. (1989). Protein Recognition of Immobilized Ligands. Hutchins, T.W., ed. Alan R. Liss, Inc., pp. 83-90.
  5. Sharma, K.K., et al. (2000) J. Biol. Chem. 275, 3767-3771.
  6. Claypool, S.M., et al. (2002) J. Biol. Chem. 277, 28038-28050.

Related Products:

Biotin
Biotin Labeling Reagents and Kits
Cell Surface Protein Isolation Kit


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
20219 Pierce Avidin Agarose
Formulation: Avidin on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding ≥ 20µg biotin/mL resin
5mL Product Instructions for product #20219 Pierce Avidin Agarose MSDS for product #20219 Pierce Avidin Agarose Certificate of Analysis for product #20219 Pierce Avidin Agarose $188.00

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20219B Pierce Avidin Agarose
Formulation: Avidin on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding = 20µg biotin/mL resin
Custom MSDS for product #20219B Pierce Avidin Agarose Certificate of Analysis for product #20219B Pierce Avidin Agarose
20225 Pierce Avidin Agarose
Formulation: Avidin on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding ≥ 20µg biotin/mL resin
5 x 5mL Product Instructions for product #20225 Pierce Avidin Agarose MSDS for product #20225 Pierce Avidin Agarose $573.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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