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Asp-N Protease, MS Grade


Complement tryptic-digest coverage with an enzyme that cleaves at the amino side of aspartate residues.

Asp-N Protease, MS Grade

Thermo Scientific Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

This Asp-N is a mass spectrometry (MS)-grade zinc metalloproteinase derived from a mutant strain of Pseudomonas fragi and requires a trace amount of zinc for activity. Asp-N can be used alone or in parallel with trypsin or other proteases to produce protein digests for peptide mapping and protein sequencing. Asp-N protease is suitable for either in-solution or in-gel digestion workflows. This Asp-N enzyme is packaged lyophilized (2µg).

Highlights:

  • Complementary to tryptic digests – hydrolyzes proteins specifically at the amino side of aspartate and cysteic acid residues
  • Increased sequence coverage – better protein characterization results from overlapping peptides with complementary chromatographic, ionization and fragmentation properties
  • High specific activity – greater than 20,000 units/mg protein
  • N-terminal arginine cleavage specificity – at least 90% for a complex protein sample
  • Stable – provided in a lyophilized format

Applications

  • Improved sequence coverage of protein digests
  • In-solution digestion of proteins
  • In-gel digestion of proteins

Product Details:

The endoproteinase AspN cleaves primarily at amino side of aspartate residues and cysteic acid residues that result from the oxidization of cysteine residues, generating a limited number of peptide fragments. Cleavage can also occur at glutamic residues; however, the rate of cleavage at the glutamyl residues is significantly lower than the rate of cleavage at the aspartic acid residues. AspN can efficiently digest protein in 2-20 hours at 37°C. AspN remains active under denaturing conditions such as 1M urea, 2M guanidine•HCl, 0.1% SDS, 2% CHAPS and 10% acetonitrile with optimal activity in the pH range of 6-8. This lyophilized enzyme has a mass of 27 kDa and is stable for 1 year when stored at -20°C.

General References:

  1. Simpson, Richard. (2003) Proteins and Proteomics, A Laboratory Manual, Cold Spring Harbor Press.

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Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
90053 Asp-N Protease, MS Grade
Formulation: Lyophilized enzyme powder
Sufficient For: At least 20,000 units/mg
2µg Product Instructions for product #90053 Asp-N Protease, MS Grade MSDS for product #90053 Asp-N Protease, MS Grade Certificate of Analysis for product #90053 Asp-N Protease, MS Grade $199.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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