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Anti-GST Coated Plates


An alternative to glutathione coated plates for GST protein capture.

Pierce Anti-GST Coated Plates

Thermo Scientific Pierce Anti-GST Coated Plates are polystyrene microplates coated with mouse monoclonal anti-GST antibody and pre-blocked for immediate use.

Thermo Scientific Pierce Anti-GST Coated Plates are polystyrene microplates coated with mouse monoclonal anti-GST antibody and pre-blocked for immediate use. Unlike glutathione coated plates, these anti-GST plates effectively bind both native and denatured forms of GST. In most cases, pre-purification of cell lysates is not necessary before screening and analysis of recombinant GST-tagged protein expression by ELISA using the plates.

Highlights:

  • Antibody-based – plates are coated with mouse monoclonal anti-GST antibody
  • Sensitive – detects GST fusion proteins down to 7.5ng/well
  • Flexibility – binds native or denatured forms of GST; an alternative to glutathione-coated plates
  • Ready to use – pre-blocked with SuperBlock Blocking Buffer
  • Compatibility – Thermo Scientific Cell Lysis Reagents and many other popular lysis buffers do not interfere with GST-fusion protein binding
  • Superior performance – improved signal-to-noise ratio relative to competitor's plates
  • Custom formats available – ask about our Custom Plate-Coating Service for coating 96-well and 384-well clear, white or black plates

Product Details:

Pierce Anti-GST Coated Plates binding capacity data Anti-GST Coated Plates bind GST for assay detection. The plates were assayed with purified GST that was secondarily detected using a rabbit anti-GST antibody (1µg/mL) followed by donkey anti-rabbit HRP conjugate. The signal was developed with Thermo Scientific Pierce Turbo TMB Substrate (Part No. 34022).

References:

  1. Smith, D.B. and Johnson, K.S. (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
  2. Bar-Peled, M. and Raikhel, N.V. (1996). A method for isolation and purification of specific antibodies to a protein fused to the GST. Anal. Biochem. 241, 140-142.

Related Resources:

Assay development and ELISA technical guide
GST-tagged protein technical guide

Related Products:

Pierce Glutathione Agarose and GST Purification Kits
Glutathione Coated Plates
B-PER Bacterial Protein Extraction Reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
15144 Anti-GST Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 200µL/well with mouse monoclonal anti-GST antibody and blocked at 200µL/well with SuperBlock Blocking Buffer
Sufficient For: Binding approx. 10ng of purified GST per well
5 plates Product Instructions for product #15144 Anti-GST Coated Plates, Clear, 8-Well Strip MSDS for product #15144 Anti-GST Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15144 Anti-GST Coated Plates, Clear, 8-Well Strip $167.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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