Thermo Scientific Pierce Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
The blocked magnetic bead surface is coated with anti-c-Myc antibody, a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). The Pierce Anti-c-Myc Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.
- Specific – highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity immunoprecipitation
- Convenient and fast – product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
- Low non-specific binding – stable, pre-blocked beads and specific antibody minimize off-target binding for c-Myc-tag IP or co-IP experiments
- Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)
Pierce Anti-c-Myc Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant c-Myc tagged proteins and the co-immunoprecipitation (Co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing c-Myc tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0) 50mM NaOH, or SDS-PAGE sample buffer, the protocol has been optimized for each of these conditions. Anti-c-Myc antibody can be used to detect c-Myc tagged protein by Western blot analysis.
Characteristics of Thermo Scientific Pierce Anti-c-Myc Magnetic Beads.
||High-affinity mouse IgG1 monoclonal antibody (clone 9E10) covalently coupled to the surface of blocked magnetic beads
||Superparamagnetic (no magnetic memory)
||≥10µg of GST-c-Myc (26kDa fusion protein)/mg of beads;
≥100µg of GST-c-Myc (26kDa fusion protein)/mL of bead suspension
Better immunoprecipitation results with Thermo Scientific Pierce Anti-c-Myc Magnetic Beads. Green Renilla luciferase c-Myc fusion protein was expressed in 293T cells. For IP, identical aliquots of the cell lysate were incubated in duplicate for one hour at room temperature with anti-c-Myc magnetic beads from each manufacturer. For all conditions, IP products were eluted identically using low-pH buffer. Eluted fractions (25µL each) were separated by 12% SDS-PAGE, transferred to PVDF membrane, and detected via anti-c-Myc antibody (Part No. MA1-980), goat anti-mouse secondary antibody, and chemiluminescent substrate (Part No. 34080). Note: Manufacturers supply magnetic beads slurries at different concentrations and recommend different amounts of beads per microgram of lysate for IP. For this reason, only 10µL of CST beads were used in this experiment. Nevertheless, Pierce beads provide equal or greater binding capacity, even in experiments involving carefully matched numbers of beads (data not shown).
Epitope Tag Product Selection Guide – links to antibodies, resins, magnetic beads and IP kits
Other Anti-Tag Antibodies
c-Myc-Tag Magnetic IP/Co-IP Kit
Fusion Protein Purification Resins and Kits
KingFisher Magnetic Particle Processors
Cell Lysis and Protein Extraction Reagents