Thermo Scientific Pierce ANB-NOS is a short-arm (7.7 angstrom) crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.
We no longer recommend the use of this product. A new crosslinker, LC-SDA (NHS-Diazirine), has similar reactivity but incorporates a higher efficiency photoreactive group.
- Reactive groups: NHS ester and nitrophenyl azide
- Reactive towards: amino groups and any nucleophile
- Nitro group on the phenyl azide ring shifts wavelength for photoactivation to 320-350nm, which is less damaging to proteins and nucleic acids
|Chemical structure of ANB-NOS crosslinking reagent.
- Krieg, U.C., Walter, P. and Johnson, A.E. (1986). Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle. Proc. Natl. Acad. Sci. USA 83, 8604-8606.
Crosslinker Application Guide -- search for recent literature references for this product
Overview of crosslinking
Chemistry of crosslinking
LC-SDA – more efficient diazirine analog of ANB-NOS
Crosslinkers at a glance (selection guide)