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ANB-NOS Photoreactive Crosslinker

A short-arm, amine-reactive and light-activatable crosslinker based on aryl azide chemistry.

Pierce ANB-NOS

Thermo Scientific Pierce ANB-NOS is a short-arm (7.7 angstrom) crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.

We no longer recommend the use of this product. A new crosslinker, LC-SDA (NHS-Diazirine), has similar reactivity but incorporates a higher efficiency photoreactive group.


  • Reactive groups: NHS ester and nitrophenyl azide
  • Reactive towards: amino groups and any nucleophile
  • N-5-Azido-2-nitrobenzoyloxysuccinimide
  • Nitro group on the phenyl azide ring shifts wavelength for photoactivation to 320-350nm, which is less damaging to proteins and nucleic acids

Product Details:

N-5-Azido-2-nitrobenzoyloxysuccinimide (ANB-NOS)
Chemical structure of ANB-NOS crosslinking reagent.


  1. Krieg, U.C., Walter, P. and Johnson, A.E. (1986). Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle. Proc. Natl. Acad. Sci. USA 83, 8604-8606.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

LC-SDA – more efficient diazirine analog of ANB-NOS
Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
21451 ANB-NOS
Formulation: N-5-Azido-2-nitrobenzoyloxysuccinimide; crystalline or powder solid
Sufficient For: 164mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #21451 ANB-NOS MSDS for product #21451 ANB-NOS Certificate of Analysis for product #21451 ANB-NOS $123.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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