Thermo Scientific Pierce Amino Acid Standard H is a quantitative mixture of 18 amino acids, supplied at 2.5µmol/mL each in 0.1N HCl, for use as a high-purity calibration standard for HPLC analysis of protein hydrolysates.
This quantitative solution of eighteen purified amino acids in 0.1N HCl is an ideal reference standard for calibrating amino acid analyzers and assessing the composition of hydrolyzed peptides and proteins (hydrolysates). To permit standardization of microbiological and other assays, the mixture uses only the L-form of each amino acid. The molar concentration of each amino acid is verified by conventional amino acid analysis methods, making the mixture suitable as both a qualitative and quantitative standard. The solution is provided as stable 1mL aliquots in sealed glass ampules.
- Complete mixture – contains eighteen (18) amino acids for assessment of amino acid composition of peptide hydrolysates
- Quantitative – contains exact amounts of each amino acid (2.5 ± 0.1µmol/mL, except cystine at 1.25 ± 0.1µmol/mL), enabling its use as a quantitation standard
- Stable – dissolved in 0.1N hydrochloric acid and stabilized as 1mL aliquots in sealed glass ampules
- Standard H – designed for compositional amino acid analysis by HPLC methods of protein or peptide hydrolysates
Composition of the Thermo Scientific Pierce Amino Acid Standard H.
When kept frozen, an unopened vial has an indefinite storage life. Once the seal is broken, the reagent has a maximum storage life of six months (store frozen between uses). To use the product, thaw a vial and shake well to ensure a homogeneous solution. Then dilute appropriately with suitable buffers to a concentration compatible with the full-scale sensitivity of your amino acid analyzer.
Trifluoroacetic Acid (TFA)