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Amino Acid Standard H

High purity amino acid calibration standard for protein hydrolyzates.

Pierce Amino Acid Standard H

Thermo Scientific Pierce Amino Acid Standard H is a quantitative mixture of 18 amino acids, supplied at 2.5µmol/mL each in 0.1N HCl, for use as a high-purity calibration standard for HPLC analysis of protein hydrolysates.

This quantitative solution of eighteen purified amino acids in 0.1N HCl is an ideal reference standard for calibrating amino acid analyzers and assessing the composition of hydrolyzed peptides and proteins (hydrolysates). To permit standardization of microbiological and other assays, the mixture uses only the L-form of each amino acid. The molar concentration of each amino acid is verified by conventional amino acid analysis methods, making the mixture suitable as both a qualitative and quantitative standard. The solution is provided as stable 1mL aliquots in sealed glass ampules.


  • Complete mixture – contains eighteen (18) amino acids for assessment of amino acid composition of peptide hydrolysates
  • Quantitative – contains exact amounts of each amino acid (2.5 ± 0.1µmol/mL, except cystine at 1.25 ± 0.1µmol/mL), enabling its use as a quantitation standard
  • Stable – dissolved in 0.1N hydrochloric acid and stabilized as 1mL aliquots in sealed glass ampules
  • Standard H – designed for compositional amino acid analysis by HPLC methods of protein or peptide hydrolysates

Product Details:

Composition of the Thermo Scientific Pierce Amino Acid Standard H.
Ammonia ([NH4]2SO4)
L-Aspartic Acid
L-Glutamic Acid

When kept frozen, an unopened vial has an indefinite storage life. Once the seal is broken, the reagent has a maximum storage life of six months (store frozen between uses). To use the product, thaw a vial and shake well to ensure a homogeneous solution. Then dilute appropriately with suitable buffers to a concentration compatible with the full-scale sensitivity of your amino acid analyzer.

Related Products:

Trifluoroacetic Acid (TFA)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
20088 Amino Acid Standard H
Formulation: Solution of purified ammonium sulfate and 17 other amino acids in 0.1N HCl
Sufficient For: Preparing hundreds of standards for HPLC experiments
10 x 1mL MSDS for product #20088 Amino Acid Standard H Certificate of Analysis for product #20088 Amino Acid Standard H $111.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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