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Amine-reactive, 10-plex Tandem Mass Tag Reagents


Measure protein expression in ten conditions simultaneously.

TMT10plex Tandem Mass Tag Reagents

Amine-reactive Thermo Scientific TMT10plex™ Isobaric Mass Tag Labeling Kits and Reagents enable multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS).

This Tandem Mass Tag™ (TMT™) 10-plex reagent set contains ten different isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm and a mass reporter. The reagent set enables up to ten different peptide samples prepared from cells or tissues to be labeled in parallel and then combined for analysis. For each sample, a unique reporter mass (i.e., TMT10 126-131Da) in the low-mass region of the high-resolution MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation and tandem mass spectrometry.

Highlights:

  • Powerful – concurrent MS analysis of multiple samples increases sample throughput and enables relative quantitation of up to ten different samples derived from cells, tissues or biological fluids
  • Consistent – identical reagent structure and performance among TMTzero™, TMTduplex™, TMTsixplex™ and TMT10plex™ reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and datasets
  • Robust – increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
  • Efficient – amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
  • Compatible – optimized for use with high resolution Thermo Scientific MS/MS platforms, such as the Q Exactive, Orbitrap Elite™ and Orbitrap Fusion™ Tribrid™ instruments with data analysis fully supported by Proteome Discoverer™ 1.4
Video of Amine-reactive TMT10plex Isobaric Mass Tagging Kit in action!

Applications:

  • Protein identification and quantitation from multiple samples of cells, tissue or biological fluids
  • Protein expression profiling of normal vs. disease states or control vs. treated
  • Multiplex up to ten different samples concurrently in a single experiment
  • Quantitative analysis of proteins for which no antibodies are available
  • Identification and quantitation of membrane and post-translationally modified proteins
  • Identification and quantification of hundreds to thousands of proteins in a single experiment

Product Details:

The Tandem Mass Tag (TMT) Reagents are specially designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The TMT10plex Label Reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex Reagents but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter. The different isotopes result in a 10-plex set of tags that have mass differences in the reporter that can be detected using high resolution Orbitrap MS instruments.

TMT chemical structures for tandem mass tagging of amine groups
Structural design of the amine-reactive Tandem Mass Tag™ Reagents.
A. Functional regions of the reagent structure including MS/MS fragmentation sites by higher energy collision dissociation (HCD) and electron transfer dissociation (ETD). B. TMT10plex reagent structures with 13C and 15N heavy isotope positions (red asterisks).

Advantages of the TMT10plex Label Reagents include increased multiplex relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. TMT10plex Label Reagents are ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments or biological replicates.

Procedure summary for parallel isobaric labeling for tandem mass spectrometry
Analysis protocol summary for tandem mass spectrometry with 6-plex isobaric tags
Procedure summary for MS experiments with Thermo Scientific TMT Isobaric Mass Tagging Reagents. Protein extracts isolated from cells or tissues are reduced, alkylated and digested overnight. Samples are labeled with the TMT Reagents and then mixed before sample fractionation and clean up. Labeled samples are analyzed by high resolution Orbitrap LC-MS/MS before data analysis to identify peptides and quantify reporter ion relative abundance.

TMT Reagents are provided as standalone sets or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience and reliability. When combined with the industry-leading, high resolution Thermo Scientific Orbitrap instruments and software, TMT10plex Reagents provide integrated total solutions for quantitative protein expression analysis.

Relative quantitation by tandem mass spectrometry with TMT10plex Reagents
Example of 10-plex relative quantitation using Thermo Scientific TMT10plex Reagents. BSA tryptic digests labeled with TMT10plex reagents (TMT10 126-131Da ) were mixed 16:8:4:2:1:1:2:4:8:16 and analyzed by high resolution (Oribitrap LC-MS). The relative abundance of the target protein or peptide fragment in ten different samples is easily measured by comparing the reporter ions generated by MS/MS fragmentation of the different mass tags.

These products are subject to a limited use label license.

References:

  1. Thompson, A., et al. (2003). Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS. Anal. Chem. 75(8): 1895-1904.
  2. McAllister, G.C., et al. (2012). Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses. Anal. Chem. 84(17): 7469-78.
  3. Werner, T., et al (2012). High-resolution enabled TMT 8‑plexing. Anal Chem. 2012. 84(16):7188-94.

Related Resources:

Mass Spec Sample Prep Workflow (selection guide)
Mass spectrometry technical guide
Quantitative proteomics technical guide
Service providers that use our mass tagging products
TMT Technology - opens Proteome Sciences website

Related Products:

Other TMT Reagents
SILAC Protein Quantitation Kits
Ubiquitin, Glycoprotein and Phosphoprotein Enrichment Kits
Fe-NTA, TiO2, C-18 and Graphite peptide enrichment and clean-up kits
Protease and Phosphatase Inhibitor Cocktails and Tablets


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
90113 TMT10plex Isobaric Mass Tag Labeling Kit
Formulation: Multi-component kit with set of ten TMT10 label reagents (3 x 0.8mg each)
Sufficient For: Three 10-plex (3 x 10-way) experiments

Kit Contents:
TMT10-126 Label Reagent, 3 x 0.8mg
TMT10-127N Label Reagent, 3 x 0.8mg
TMT10-127C Label Reagent, 3 x 0.8mg
TMT10-128N Label Reagent, 3 x 0.8mg
TMT10-128C Label Reagent, 3 x 0.8mg
TMT10-129N Label Reagent, 3 x 0.8mg
TMT10-129C Label Reagent, 3 x 0.8mg
TMT10-130N Label Reagent, 3 x 0.8mg
TMT10-130C Label Reagent, 3 x 0.8mg
TMT10-131 Label Reagent, 3 x 0.8mg
Dissolution Buffer (1M triethyl ammonium bicarbonate), 5mL
Denaturing Reagent (10% SDS), 1mL
Reducing Reagent (0.5M TCEP), 1mL
Iodoacetamide, 12 × 9mg
Quenching Reagent (50% hydroxylamine), 1mL
Pierce Trypsin Protease, MS Grade, 5 × 20µg
Trypsin Storage Solution, 250μL
Albumin, Bovine, 2.5mg
30-rxn kit Product Instructions for product #90113 TMT10plex Isobaric Mass Tag Labeling Kit MSDS for product #90113 TMT10plex Isobaric Mass Tag Labeling Kit Certificate of Analysis for product #90113 TMT10plex Isobaric Mass Tag Labeling Kit $2,800.00

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90110 TMT10plex Isobaric Label Reagent Set, 1 x 0.8mg
Formulation: Set of ten TMT10 label reagents (1 x 0.8mg each)
Sufficient For: One 10-plex (1 x 10-way) experiment

Kit Contents:
TMT10-126 Label Reagent, 1 x 0.8mg
TMT10-127N Label Reagent, 1 x 0.8mg
TMT10-127C Label Reagent, 1 x 0.8mg
TMT10-128N Label Reagent, 1 x 0.8mg
TMT10-128C Label Reagent, 1 x 0.8mg
TMT10-129N Label Reagent, 1 x 0.8mg
TMT10-129C Label Reagent, 1 x 0.8mg
TMT10-130N Label Reagent, 1 x 0.8mg
TMT10-130C Label Reagent, 1 x 0.8mg
TMT10-131 Label Reagent, 1 x 0.8mg
10-rxn set Product Instructions for product #90110 TMT10plex Isobaric Label Reagent Set, 1 x 0.8mg MSDS for product #90110 TMT10plex Isobaric Label Reagent Set, 1 x 0.8mg Certificate of Analysis for product #90110 TMT10plex Isobaric Label Reagent Set, 1 x 0.8mg $950.00

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90111 TMT10plex Isobaric Label Reagent Set, 3 x 0.8mg
Formulation: Set of ten TMT10 label reagents (3 x 0.8mg each)
Sufficient For: Three 10-plex (3 x 10-way) experiments

Kit Contents:
TMT10-126 Label Reagent, 3 x 0.8mg
TMT10-127N Label Reagent, 3 x 0.8mg
TMT10-127C Label Reagent, 3 x 0.8mg
TMT10-128N Label Reagent, 3 x 0.8mg
TMT10-128C Label Reagent, 3 x 0.8mg
TMT10-129N Label Reagent, 3 x 0.8mg
TMT10-129C Label Reagent, 3 x 0.8mg
TMT10-130N Label Reagent, 3 x 0.8mg
TMT10-130C Label Reagent, 3 x 0.8mg
TMT10-131 Label Reagent, 3 x 0.8mg
30-rxn set Product Instructions for product #90111 TMT10plex Isobaric Label Reagent Set, 3 x 0.8mg MSDS for product #90111 TMT10plex Isobaric Label Reagent Set, 3 x 0.8mg Certificate of Analysis for product #90111 TMT10plex Isobaric Label Reagent Set, 3 x 0.8mg $2,575.00

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90406 TMT10plex Isobaric Label Reagent Set, 1 x 5mg
Formulation: Set of ten TMT10 label reagents (1 x 5mg each)
Sufficient For: Six 10-plex (6 x 10-way) experiments

Kit Contents:
TMT10-126 Label Reagent, 1 x 5mg
TMT10-127N Label Reagent, 1 x 5mg
TMT10-127C Label Reagent, 1 x 5mg
TMT10-128N Label Reagent, 1 x 5mg
TMT10-128C Label Reagent, 1 x 5mg
TMT10-129N Label Reagent, 1 x 5mg
TMT10-129C Label Reagent, 1 x 5mg
TMT10-130N Label Reagent, 1 x 5mg
TMT10-130C Label Reagent, 1 x 5mg
TMT10-131 Label Reagent, 1 x 5mg
60-rxn set Product Instructions for product #90406 TMT10plex Isobaric Label Reagent Set, 1 x 5mg MSDS for product #90406 TMT10plex Isobaric Label Reagent Set, 1 x 5mg Certificate of Analysis for product #90406 TMT10plex Isobaric Label Reagent Set, 1 x 5mg $4,500.00

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90114 1M Triethylammonium bicarbonate (TEAB)
Formulation: 10X dissolution buffer for isobaric mass tag labeling experiments
Sufficient For: 500mL of 1X (0.1M) TEAB solution
50mL MSDS for product #90114 1M Triethylammonium bicarbonate (TEAB) Certificate of Analysis for product #90114 1M Triethylammonium bicarbonate (TEAB) $95.00

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90115 50% Hydroxylamine
Formulation: 10X quenching reagent for amine-reactive isobaric mass tag labeling experiments
Sufficient For: 50mL of 1X (5%) hydroxylamine solution
5mL MSDS for product #90115 50% Hydroxylamine Certificate of Analysis for product #90115 50% Hydroxylamine $60.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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