Thermo Scientific Pierce AMAS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a very short spacer arm (4.4 angstroms).
- Reactive groups: NHS ester and maleimide
- Reactive towards: amino and sulfhydryl groups
- Shortest of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
- NHS ester end couples with primary amines at pH 7-9, forming stable amide bonds
- Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
- Water-insoluble (dissolve first in DMF or DMSO)
- Very short aliphatic spacer has low potential for eliciting an immune response
|Chemical structure of AMAS crosslinking reagent.
- May, J.M. (1989). Selective labeling of the erythrocyte hexose carrier with a maleimide derivative of glucosamine: relationship of an exofacial sulfhydryl to carrier conformation and structure. Biochemistry 28, 1718-1725.
- Sayre, L.M., Larson, D.L., Takemori, A.E. and Portoghese, P.S. (1984). Design and synthesis of naltrexone-derived affinity labels with nonequilibrium opioid agonist and antagonist activities. Evidence for the existence of different mu receptor subtypes in different tissues. J. Med. Chem. 27(10), 1325-1335.
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