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AMAS N-(a-Maleimidoacetoxy) succinimide ester)

An amine-to-sulfhydryl crosslinker with a very short spacer arm (4.4 angstroms).

Pierce AMAS

Thermo Scientific Pierce AMAS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a very short spacer arm (4.4 angstroms).


  • Reactive groups: NHS ester and maleimide
  • Reactive towards: amino and sulfhydryl groups
  • Shortest of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
  • NHS ester end couples with primary amines at pH 7-9, forming stable amide bonds
  • Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO)
  • Very short aliphatic spacer has low potential for eliciting an immune response

Product Details:

Pierce AMAS
Chemical structure of AMAS crosslinking reagent.


  1. May, J.M. (1989). Selective labeling of the erythrocyte hexose carrier with a maleimide derivative of glucosamine: relationship of an exofacial sulfhydryl to carrier conformation and structure. Biochemistry 28, 1718-1725.
  2. Sayre, L.M., Larson, D.L., Takemori, A.E. and Portoghese, P.S. (1984). Design and synthesis of naltrexone-derived affinity labels with nonequilibrium opioid agonist and antagonist activities. Evidence for the existence of different mu receptor subtypes in different tissues. J. Med. Chem. 27(10), 1325-1335.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22295 AMAS
Formulation: N-alpha-Maleimidoacet-oxysuccinimide ester; solid
Sufficient For: 198mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22295 AMAS MSDS for product #22295 AMAS Certificate of Analysis for product #22295 AMAS $112.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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