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Alkaline Phosphatase (AP)


A responsive enzyme-reporter for ELISA and immunohistochemistry applications.

Alkaline phosphatase

Thermo Scientific Pierce Alkaline Phosphatase is purified calf-intestinal alkaline phosphatase (AP or alk-phos) enzyme for use in activity assays and conjugation to antibodies for ELISA and immunohistochemistry applications.

This purified calf intestinal alkaline phosphatase (CIP) is supplied in Tris buffer and 50% glycerol for use in protein research methods. The main applications for alkaline phosphatase in molecular biology and protein research are to remove 5'-phosphate groups from DNA or as a reporter system for immunoassays such as ELISA. For these latter methods, the enzyme is usually conjugated to specific primary or secondary antibodies and its activity is detected with a color-forming (or light-generating) substrate.

Highlights:

  • Purified form – ready to dilute and conjugate without prior dialysis
  • Concentrated – approximately 20mg/mL (lot-specific value reported)
  • High specific activity – typically greater than 1600 units/mg (lot-specific value reported)
  • Tris buffered solution – supplied in 5mM Tris, 5mM magnesium chloride and 0.1mM zinc chloride,
    pH ~7.0 in 50% glycerol

Product Details:

One unit equals the amount of protein required to hydrolyze one micromole of p-nitrophenyl phosphate (PNPP) per minute at 25°C in a glycine buffer, pH 9.6.

General References:

  1. Bulman, A.S. and Heyderman, E. (1981). Alkaline phosphatase for immunocytochemical labeling: problems with endogenous enzyme activity. J. Clin. Pathol. 34, 1349-1351.
  2. Cordell, J.L., et al. (1984). Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Histochem. Cytochem. 32, 219-229.
  3. Yolken, R.H. (1982). Enzyme immunoassays for the detection of infectious agents in body fluids: current limitations and future prospects. Rev. Infect. Dis. 4, 35-68.

Related Resources:

Immunohistochemistry (IHC) technical guide
Immunodetection for IHC technical guide

Related Products:

Substrates for ELISA Detection
Substrates for Western Blot Detection
PNPP (p-Nitrophenyl Phosphate, Disodium Salt) Substrates
NBT/BCIP Substrates


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
31391 Alkaline Phosphatase
Formulation: Calf intestinal alkaline phosphatase in Tris buffered saline and 50% glycerol
Sufficient For: Several to many uses depending on the application
20mg MSDS for product #31391 Alkaline Phosphatase Certificate of Analysis for product #31391 Alkaline Phosphatase $860.00

Add to cart
31392 Alkaline Phosphatase
Formulation: Calf intestinal alkaline phosphatase in Tris buffered saline and 50% glycerol
Sufficient For: Many uses depending on the application
5 x 20mg MSDS for product #31392 Alkaline Phosphatase $3,247.00

Add to cart
31392B Alkaline Phosphatase
Formulation: Calf intestinal alkaline phosphatase in Tris buffered saline and 50% glycerol
Custom MSDS for product #31392B Alkaline Phosphatase Certificate of Analysis for product #31392B Alkaline Phosphatase

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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