Thermo Scientific Pierce AEDP is an amine-and-carboxyl compound with an internal disulfide bond. AEDP enables construction, extension and modification of several functional groups on proteins and surfaces.
AEDP (3-[(2-aminoethyl)dithio]propionic acid•HCl) is a cleavable amino acid that can be reacted with amine-reactive acylation agents to create amide bonds. Alternatively, the carbodiimide EDC can be used to conjugate AEDP to molecules containing amines or carboxylates, which results in some degree of polymerization. Reducing agents, such as DTT and TCEP, are effective for cleaving the disulfide bond.
- Use as a spacer arm in conjugation schemes
- Use for reversible immobilization applications
- Cleave the internal disulfide bond with reducing agents
- Incorporate amine or carboxyl groups into proteins/peptides using crosslinking agents
- React the amine end with amine-reactive acylation agents
- Use with water-soluble carbodiimide (EDC) to conjugate to amines or carboxylates
Structure and properties of AEDP:
- Functional groups: primary amine and carboxyl; disulfide bond
- Chemical name: 3-[(2-aminoethyl)dithio]propionic acid•HCl
- Chemical formula: C5H12ClNO2S2
- Molecular weight: 217.74
- Form: white to off-white solid, free of foreign material
- Schnaar, R.L., et al. (1985). Reversible covalent immobilization of ligands and proteins on polyacrylamide gels. Anal Biochem 151:268-81.
- Sayre, L.M., et al. (1984). Design and synthesis of naltrexone-derived affinity labels with nonequilibrium opoid agonist and antagonist activities. Evidence for the existence of different mu receptor subtypes in different tissues. J Med Chem 27(10):1325-35.
Overview of crosslinking and protein modification