Update profile
    Change password
    Sign out

Our site will automatically redirect starting in March. Bookmark our protein biology page on lifetechnologies.com  Go now >

ABTS Substrate

HRP substrate for ELISA plate reader detection at 410nm or 650nm.

ABTS Substrates

Thermo Scientific Pierce ABTS Substrates are tablets and a ready-to-use solution of a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods.

ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410nm and 650nm. ABTS is less sensitive than OPD and TMB in ELISA applications. It is less readily oxidized, and its color development is slower(approximately 20 minutes). This may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities.

Highlights (1-Step ABTS Solution):

  • One component
  • Ready-to-use
  • Excellent choice when maximum sensitivities are not required


  1. Paing, M.M., et al. (2002). J. Biol. Chem. 277: 1292-1300.
  2. Sau-Ching Wu, S.-C., et al. (2002). Appl. Envir. Microbiol. 68: 3261-9.

Related Resources:

Assay development and ELISA technical guide
ELISA substrate selection guide

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
34026 ABTS Tablets
Formulation: Tablets each containing 10mg of ABTS diammonium salt with inert filler as the balance
Sufficient For: 10mL of typical 1X substrate solution per tablet (500mL total; approx. 3300 wells at 150μL per well)
50 tablets Product Instructions for product #34026 ABTS Tablets MSDS for product #34026 ABTS Tablets Certificate of Analysis for product #34026 ABTS Tablets $142.00

Now available on lifetechnologies.com
37615 1-Step ABTS Solution
Formulation: Ready-to-use (1X) proprietary buffered solution of ABTS and peroxide
Sufficient For: 1600 wells at 150μL per well
250mL Product Instructions for product #37615 1-Step ABTS Solution MSDS for product #37615 1-Step ABTS Solution Certificate of Analysis for product #37615 1-Step ABTS Solution $93.00

Now available on lifetechnologies.com

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
34080 - SuperSignal West Pico Chemiluminescent Substrate

34076 - SuperSignal West Dura Extended Duration Substrate

32106 - Pierce ECL Western Blotting Substrate

34075 - SuperSignal West Dura Extended Duration Substrate

34090 - CL-XPosure Film, 5 x 7in. (13 x 18cm)


Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2015 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747