Thermo Scientific Pierce ABTS Substrates are tablets and a ready-to-use solution of a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods.
ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410nm and 650nm. ABTS is less sensitive than OPD and TMB in ELISA applications. It is less readily oxidized, and its color development is slower(approximately 20 minutes). This may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities.
Highlights (1-Step ABTS Solution):
- One component
- Excellent choice when maximum sensitivities are not required
- Paing, M.M., et al. (2002). J. Biol. Chem. 277: 1292-1300.
- Sau-Ching Wu, S.-C., et al. (2002). Appl. Envir. Microbiol. 68: 3261-9.
Assay development and ELISA technical guide
ELISA substrate selection guide