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ABC Staining Kits


Highly sensitive immunohistochemistry detection for biotinylated antibodies.

Pierce ABC Staining Kits

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.

Related Resources:

Immunohistochemistry (IHC) technical guide
IHC immunodetection strategies technical guide


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
32020 ABC Peroxidase Standard Staining Kit
Formulation: Two-reagent kit with avidin and biotinylated HRP
Sufficient For: At least 10mL of ABC staining solution

Kit Contents:
Reagent A (Avidin), 2mL
Reagent B (Biotinylated HRP), 2mL
Mixing bottle with dropper dispenser tip, 1
4mL kit Product Instructions for product #32020 ABC Peroxidase Standard Staining Kit MSDS for product #32020 ABC Peroxidase Standard Staining Kit Certificate of Analysis for product #32020 ABC Peroxidase Standard Staining Kit $299.00

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32050 Ultra-Sensitive ABC Peroxidase Standard Staining Kit
Formulation: Two-reagent kit with avidin and biotinylated HRP
Sufficient For: At least 10mL of ABC staining solution

Kit Contents:
Reagent A (Avidin), 2mL
Reagent B (Biotinylated HRP), 2mL
Mixing bottle with dropper dispenser tip, 1
4mL kit Product Instructions for product #32050 Ultra-Sensitive ABC Peroxidase Standard Staining Kit MSDS for product #32050 Ultra-Sensitive ABC Peroxidase Standard Staining Kit Certificate of Analysis for product #32050 Ultra-Sensitive ABC Peroxidase Standard Staining Kit $388.00

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32052 Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit
Formulation: Four-reagent kit including anti-mouse secondary antibody
Sufficient For: At least 10mL of ABC staining solution

Kit Contents:
Reagent A (Avidin), 2mL
Reagent B (Biotinylated HRP), 2mL
Blocking Serum (horse), 3mL
Biotinylated Horse Anti-Mouse IgG, 1mL
Mixing bottles with dropper dispenser tips, 3
8mL kit Product Instructions for product #32052 Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit MSDS for product #32052 Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit Certificate of Analysis for product #32052 Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit $554.00

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32054 Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit
Formulation: Four-reagent kit including anti-rabbit secondary antibody
Sufficient For: At least 10mL of ABC staining solution

Kit Contents:
Reagent A (Avidin), 2mL
Reagent B (Biotinylated HRP), 2mL
Blocking Serum (goat), 3mL
Biotinylated Goat Anti-Rabbit IgG, 1mL
Mixing bottles with dropper dispenser tips, 3
8mL kit Product Instructions for product #32054 Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit MSDS for product #32054 Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit Certificate of Analysis for product #32054 Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit $542.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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