Thermo Scientific Pierce 2-Mercaptoethanol (2-ME), also called beta-mercaptoethanol (b-ME), is a mild reducing agent for cleaving protein disulfide bonds.
- Also known as β-mercaptoethanol, beta-mercaptoethanol, bME, b-ME
- Mild but effective reducing agent, often included in enzyme solutions to protect against catalytic site inactivation due to cysteine sulfhydryl oxidation/disulfide formation; added at final concentrations of 5 and 20mM, with or without EDTA, as an additional protectant
Overview of crosslinking and protein modification
Overview of protein electrophoresis
All Disulfide Reducing Agents
Ellman’s Reagent – measure sulfhydryls
Electrophoresis Sample Loading Buffers