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Pierce 1-Step TMB-Blotting Substrate Solution

A precipitating form of TMB HRP substrate for Western blotting.

Pierce 1-Step TMB-Blotting Solution

Thermo Scientific Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blot and IHC experiments.

TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.


  • HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media
  • Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates
  • Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use
  • Complete – does not require other buffers or addition of hydrogen peroxide


  • For best results, use TMB-Blotting Substrate with blots that have been blocked with Thermo Scientific SuperBlock Blocking Buffers for Blotting (Part No. 37517, 37537)

Related Resources:

Chromogenic Western blotting technical guide

Related Products:

SuperBlock Blocking Buffers for Blotting
1-Step Ultra TMB-Blotting Solution
Soluble TMB Substrates for ELISA

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
34018 1-Step TMB-Blotting Solution
Formulation: 3,3',5,5'-Tetramethylbenzidine in proprietary buffer containing peroxide
Sufficient For: 25 uses at 10mL each
250mL Product Instructions for product #34018 1-Step TMB-Blotting Solution MSDS for product #34018 1-Step TMB-Blotting Solution Certificate of Analysis for product #34018 1-Step TMB-Blotting Solution $123.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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