Subject Search: Western Blotting

Transfer EMSA gels using the Pierce G2 Fast Blotter

The Pierce G2 Fast Blotter (Part No. 62288) is a device designed primarily for semi-dry transfer of protein gels for Western blotting. We were interested to know if the same device would work effectively to transfer DNA-based polyacrylamide gels for performing membrane-based chemiluminescent electrophoretic mobility shift assays (EMSA). We compared the Pierce G2 Fast Blotter

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Improved Western blot detection using CCD digital imaging

Chemiluminescent Western blotting is a popular protein detection method for studying proteins. The typical protocol for measuring chemiluminescent signal exposes the Western blot to laboratory-grade X-ray film, but digital imaging using a cooled-CCD camera is proving to be a more functional choice for researchers. The Thermo Scientific myECL Imager offers digital imaging with a number

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Western blotting using enhanced chemiluminescence

Chemiluminescence is the most popular method of detection in Western blotting applications because it offers several advantages over precipitating substrates, including high sensitivity, high signal-to-noise ratios, and the ability to strip and reprobe the blot. Additionally, chemiluminescent detection can be quantitative when blots are analyzed using CCD-based imagers.1 Among chemiluminescent substrates, the luminol-based substrates that

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Pierce ECL Plus Western Blotting Substrate

The Thermo Scientific Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent peroxidase substrate for Western blotting. The Pierce ECL Plus Substrate generates acridinium esters when it reacts with horseradish peroxidase (HRP), producing a strong signal that is equal to or better than similar commercially available substrates (Figure 1). The ester intermediates react with peroxide

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Previews Volume 14, Issue 3

This 2010 issue of the Previews newsletter features articles about new kits and products for kinase inhibitor research, phosphopeptide enrichment, GST-fusion protein purification, assessing molecular weight and enhancing signal in Western blotting, and choosing carrier proteins for antibody production.

Previews was a print magazine that highlighted new Pierce Protein Research products and applications. Articles included

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Previews Volume 13, Issue 2

This 2009 issue of the Previews newsletter features articles about new kits and products for fast Western blotting, in-cell ELISA, fluorescent antibody detection, biotin methods, and immunoprecipitation.

Previews was a print magazine that highlighted new Pierce Protein Research products and applications. Articles included information relating to product detection, purification, modification, analysis and more. Previews has

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