Subject Search: Sample Preparation

Mass spectrometry sample preparation procedure for protein samples

Mass spectrometry (MS) has become a prominent technique in biological research for the identification, characterization, and quantification of proteins (Ref. 1). Shotgun proteomics is a commonly used strategy to identify proteins in complex mixtures by digesting proteins at specific amino acids into peptides that can be separated and identified by mass spectrometry (Ref.2). In order

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Eliminate endotoxins from protein and antibody samples

Traditional endotoxin removal methods are lengthy and require significant optimization for every target molecule being purified. Furthermore, ionic methods for binding endotoxins require pH optimization to avoid binding highly charged proteins, and size-exclusion methods to filter endotoxins are not effective for large proteins. We developed an endotoxin removal resin that is effective for eliminating endotoxins

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Removing endotoxins using a spin-column format

Endotoxin removal during the development of biological therapeutics is critical for preventing inflammation, tissue death and endotoxin shock. Traditional resins that use polymixin-B to remove endotoxins have low binding efficiency. Using gravity-flow chromatography can improve binding by increasing the contact time of the resin with the endotoxin-containing sample. As with most biological samples, however, minimizing

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Protease and phosphatase inhibitor tablets

Proteases and phosphatases serve many metabolic and regulatory functions; however, upon cell lysis, the once compartmentally contained enzymes are capable of mass protein degradation and dephosphorylation. The preservation of proteins from protease and phosphatase activities after cell or tissue lysis is essential for many research applications. The Thermo Scientific Pierce Protease, Phosphatase, and combination Protease

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Low-volume sample dialysis

The Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices are a convenient and easy means for dialyzing small-volume protein samples (10-100μL). Our new the 0.5mL and 2mL Slide-A-Lyzer* MINI Dialysis Devices can process 50-500μL and 200-2000μL samples, respectively, and are self-contained, single-use devices that require no syringes, centrifuge, beakers or laborious steps. Sample addition and removal are

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Previews Volume 14, Issue 3

This 2010 issue of the Previews newsletter features articles about new kits and products for kinase inhibitor research, phosphopeptide enrichment, GST-fusion protein purification, assessing molecular weight and enhancing signal in Western blotting, and choosing carrier proteins for antibody production.

Previews was a print magazine that highlighted new Pierce Protein Research products and applications. Articles included

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Previews Volume 13, Issue 3

This 2009 issue of the Previews newsletter features articles about new kits and products for dialysis, protein concentration, desalting, peptide clean-up for mass spectrometry, detergent removal, glycoprotein labeling and detection, fluorescent labeling, and in-cell ELISA.

Previews was a print magazine that highlighted new Pierce Protein Research products and applications. Articles included information relating to product

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Previews Volume 13, Issue 1

This 2009 issue of the Previews newsletter features articles about new kits and products for tandem mass tagging for mass spectrometry, subcellular protein fractionation, competition equilibrium dialysis, and immunodetection with poly-HRP conjugates.

Previews was a print magazine that highlighted new Pierce Protein Research products and applications. Articles included information relating to product detection, purification, modification,

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