Subject Search: Protein Immobilization

Improved immobilization and conjugation of glycoproteins

Aldehyde-hydrazide chemistry has been used for over 30 years for coupling oxidized glycoproteins to activated agarose supports. This chemistry is pH- and time-sensitive, and can result in low yields even after 48 hours of incubation. We have optimized the buffer conditions and added a catalyst to reduce the reaction time while maximizing coupling.

The Thermo

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Previews Volume 14, Issue 1

This 2010 issue of the Previews newsletter features articles about new kits and products for chromatin immunoprecipitation (ChIP assays), Ni-NTA His-tagged protein purification, protein immobilization to NHS-activated agarose, graphite-based phosphopeptide purification, and cell lysis for IP.

Previews was a print magazine that highlighted new Pierce Protein Research products and applications. Articles included information relating to

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