Subject Search: Protein Assays

Serine hydrolase active-site probes

The Thermo Scientific ActivX Serine Hydrolase Probes consist of a tag linked to a fluorophosphonate (FP) group, which specifically and covalently labels serines of enzymatically active serine hydrolases (Figure 1).1-3 In addition to activity assessment, FP probes can be used to screen small-molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant

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Measure small GTPase activation

The Ras superfamily of small GTPases serve as molecular switches to control diverse eukaryotic cellular behaviors, including cell growth, differentiation and motility. Consequently, small GTPases are involved in several disease states such as cancer and metabolic disorders.1,2 GTPases are active when bound to guanosine triphosphate (GTP) and inactive when the triphosphate is hydrolyzed to guanosine

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