Subject Search: Post-translational Modification

Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagents

S-nitrosylation is a post-translational modification (PTM) that regulates numerous processes, including cellular proliferation, apoptosis, smooth muscle relaxation, neurotransmitter release, and differentiation (Ref.1). During S-nitrosylation, nitric oxide radicals react with free cysteine thiols to produce an S-NO adduct that alters protein activity. Unlike other PTMs such as phoshorylation and acetylation, S-nitrosylation does not appear to have

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Quantitative cancer stem cell phosphoprotein profiling

Cancer stem cells (CSCs) are hypothesized to provide a repository of cells that are refractory to chemotherapeutic agents developed for treating differentiated tumor cells. (Ref.1) A glioblastoma cancer stem cell line (NSC11) differentiates from a pluripotent state to a progenitor cell-like state when stimulated with WP1193, an inhibitor of STAT3 phosphorylation. (Ref.2) In this study,

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Phosphoprotein enrichment from cell and tissue samples

Reversible protein phosphorylation regulates nearly all intracellular biological events, including signal transduction, protein-protein interactions, protein stability, protein localization, apoptosis and cell-cycle control. Deregulation of protein phosphorylation is a hallmark of numerous human diseases, including cancer and metabolic and immune disorders. However, poorly developed phospho-specific antibodies, the labile state of the phosphate group, and low phosphoprotein

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Protease and phosphatase inhibitor tablets

Proteases and phosphatases serve many metabolic and regulatory functions; however, upon cell lysis, the once compartmentally contained enzymes are capable of mass protein degradation and dephosphorylation. The preservation of proteins from protease and phosphatase activities after cell or tissue lysis is essential for many research applications. The Thermo Scientific Pierce Protease, Phosphatase, and combination Protease

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