Subject Search: Lysis and Extraction

One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Cultured primary cardiomyocytes are widely used to study and understand the mechanisms underlying normal cardiovascular function, cardioprotection, and cardiovascular diseases. Cardiomyocyte cultures provide a homogeneous population of single cells, which are easy to visualize and manipulate. Compared to the whole heart, cardiomyocyte cultures are relatively pure, with limited contaminating cell types such as endothelial cells.

Continue reading…

Improved, all-in-one B-PER Reagent for bacterial protein extraction

Gram-positive Bacilli, Lactobacilli and Gram-negative E. coli are favorite recombinant protein production systems due to their simple cultivation and easy genetic manipulation. In these prokaryotic recombinant cell factories, the accumulation of target product can be secreted into the cultivation media or accumulated inside of the cell. In Gram-negative bacteria such as E. coli there is

Continue reading…

An efficient method for the isolation of highly functional primary neurons

Primary neuron cultures are powerful model systems used to study neuron morphology and differentiation, synaptic function and neurotransmitter release, neurotoxicity during pre-clinical drug development, and disease modeling. Because primary neuron cultures eliminate many of the complications present in whole animal models, there are advantages to using them in electrophysiological recording and stimulation experiments, pharmacological manipulations,

Continue reading…

Efficient mammalian membrane protein extraction

Integral and peripheral membrane proteins (MPs) are important for the maintenance of many cellular functions such as signal transduction, cell integrity, intracellular and extracellular transport of molecular solutes and cell-to-cell communication. The physical nature of the association between integral or peripheral MPs and the membrane phospholipid bilayer is significantly different. Integral MPs span the entire

Continue reading…

Subcellular protein fractionation

Proteins within a cell are localized to specific cellular compartments that provide crucial information about the function of the protein. The study of protein localization and translocation is increasingly important for understanding protein functions and diseases, especially cancers. Research is moving from cultured cell models to animal models for a more true representation of the

Continue reading…

Protease and phosphatase inhibitor tablets

Proteases and phosphatases serve many metabolic and regulatory functions; however, upon cell lysis, the once compartmentally contained enzymes are capable of mass protein degradation and dephosphorylation. The preservation of proteins from protease and phosphatase activities after cell or tissue lysis is essential for many research applications. The Thermo Scientific Pierce Protease, Phosphatase, and combination Protease

Continue reading…

Neuronal protein extraction

Neuronal tissue and primary cells are often used in neuroscience research to study proteins involved in learning, behavior and neurodegenerative disease. The Thermo Scientific N-PER Neuronal Protein Extraction Reagent is a specialized formulation for extracting total protein from neuronal tissue while maintaining protein function for downstream assays. The N-PER* Reagent typically produces better protein yield

Continue reading…

Previews Volume 13, Issue 1

This 2009 issue of the Previews newsletter features articles about new kits and products for tandem mass tagging for mass spectrometry, subcellular protein fractionation, competition equilibrium dialysis, and immunodetection with poly-HRP conjugates.

Previews was a print magazine that highlighted new Pierce Protein Research products and applications. Articles included information relating to product detection, purification, modification,

Continue reading…