Subject Search: Luciferase Assays

Luciferase assays and BioTek Synergy microplate readers

Reporter assays have long been used to investigate regulatory mechanisms of gene expression in response to environmental stimuli in a variety of organisms. Reporter constructs rely on the insertion of a genetic regulatory element upstream of a reporter gene followed by insertion of the construct, generally a plasmid, into the target cell or organism (Figure

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Versatile luciferases: new tools for reporter assays

This application note presents how Thermo Scientific microplate luminometers in conjunction with Thermo Scientific Pierce Luciferase Reporter Gene Assays form a powerful combination for reporter gene studies in high-throughput format. The focus of this application note is on introducing different luciferase reporter assay types and the requirements they place on the luminometer. It also shows

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Activation of the antioxidant response pathway by pesticide chemicals

Parkinson disease (PD) is a neurodegenerative disorder characterized by the selective loss of dopaminergic neurons and the presence of Lewy bodies in the substantia nigra pars compacta (SNpc) (Lees 2007). However, the molecular events and mechanisms involved remain unknown. Common herbicides and insecticides have been implicated in the pathogenesis of Parkinson’s disease (Tanner 2011). Paraquat

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Monitoring neuronal differentiation using multiplexed luciferase reporters

Cellular differentiation studies require sensitive tools to detect subtle phenotypic changes in cellular signaling pathways and morphology. In the present study, we used luciferase assays and high-content immunofluorescence cellular imaging to monitor phenotypic changes in neuronal precursor cell lines following retinoic acid-induced differentiation.

Retinoic Acid (RA), the biologically active form of Vitamin A, is widely

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Simultaneous dual-emission detection of luciferase reporter assays

Luciferase-based reporter assays are a powerful tool for monitoring gene expression in cells because of their ultrasensitive detection capacity and wide dynamic range. These assays involve inserting a genetic regulatory element upstream of a luciferase gene and then transferring the resulting reporter construct into animal cells, plant cells or bacteria via transfection, transformation or injection.

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Luciferase Detection Instrumentation Guide

Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. And while many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high feature variability. We therefore gathered salient information about many of the instruments available to

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Luciferase assays in Jurkat cells

The plasmid-based luciferase reporter assay is a powerful tool for studying signal transduction pathways in mammalian cells. However, cells with low transfection efficiency, resulting in low luminescent signal output, can make detection difficult. The Thermo Scientific Pierce Gaussia, Cypridina, and Green Renilla Luciferase Reporter Assays produce a robust bioluminescent signal, enabling luciferase-based pathway studies in

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Secreted dual-spectral luciferase reporter assays

The study of complex cellular signaling pathways requires powerful and specific tools to monitor changes in gene activation or repression. To accurately monitor these processes, reporter gene assays are commonly used. Our multiplexed luciferase reporters for studying gene regulation are more sensitive and convenient than luciferase reporter systems from other suppliers. We use two naturally

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