Subject Search: Immunoprecipitation

Pierce qIP Assay: a novel, HTS-compatible protein-protein interaction assay system

The study of protein interaction networks is an important approach to understanding cell biology and represents a growing interest in drug discovery. However, developing sensitive, quantitative, and high-throughput screening (HTS) methods that permit direct measurements of protein-protein interactions has proven challenging. The traditional co-immunoprecipitation approach is not practical for HTS as it requires time-consuming sample

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Webinar: Isolation and detection of protein-RNA interactions

View this recorded webinar and improve the way you isolate RNA-protein complexes. Our expert scientist discusses nonradioactive nucleic acid labeling technologies for the enrichment and detection of protein-nucleic interactions.

Dr. Kay Opperman Technical Product Manager Thermo Scientific™ Protein Biology Products

On October 29, 2013, Dr. Kay Opperman presented an overview

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ChIP analysis of androgen regulation of transcriptional activity

Many factors, including androgens, growth factors, and transcription factors, participate in the regulation of normal and cancerous prostatic development and function. The androgen receptor (AR) is a key regulator of normal prostate function and survival; however, the AR also can be converted into an inducer of uncontrolled cell growth in prostate carcinoma. This steroid receptor

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Improved immobilization and conjugation of glycoproteins

Aldehyde-hydrazide chemistry has been used for over 30 years for coupling oxidized glycoproteins to activated agarose supports. This chemistry is pH- and time-sensitive, and can result in low yields even after 48 hours of incubation. We have optimized the buffer conditions and added a catalyst to reduce the reaction time while maximizing coupling.

The Thermo

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Protein A/G magnetic beads

The Thermo Scientific Pierce Protein A/G Magnetic Beads enable efficient immunoprecipitation (IP), co-immunoprecipitation (co-IP) and antibody purification with high purity and low background. These unique 1µm particles consist of a polymeric core coated with two layers of magnetite and sealed with a blocking agent to reduce nonspecific binding (Table 1; Figure 1). The bead surface

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Measure small GTPase activation

The Ras superfamily of small GTPases serve as molecular switches to control diverse eukaryotic cellular behaviors, including cell growth, differentiation and motility. Consequently, small GTPases are involved in several disease states such as cancer and metabolic disorders.1,2 GTPases are active when bound to guanosine triphosphate (GTP) and inactive when the triphosphate is hydrolyzed to guanosine

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Previews Volume 14, Issue 1

This 2010 issue of the Previews newsletter features articles about new kits and products for chromatin immunoprecipitation (ChIP assays), Ni-NTA His-tagged protein purification, protein immobilization to NHS-activated agarose, graphite-based phosphopeptide purification, and cell lysis for IP.

Previews was a print magazine that highlighted new Pierce Protein Research products and applications. Articles included information relating to

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