Subject Search: Fusion Tag Methods

Choosing a vector and purification method for in vitro protein expression

In vitro protein expression is a rapid technique to produce functional proteins. By contrast with in vivo protein expression methods, in vitro protein expression allows for expression of toxic proteins, incorporation of heavy isotopes for NMR or MS analysis, addition of non-natural amino acids, and elimination of growth and induction conditions for recombinant protein synthesis.

Continue reading…

Performance characterization of Pierce Glutathione Superflow Agarose

The study of protein function and structure has been greatly enhanced by recombinant protein expression strategies that incorporate fusions of affinity tags to aid in purification. The glutathione S-transferase (GST) tag is particularly popular for two primary reasons: (1) the GST tag usually increases the solubility of proteins to which it is fused, thereby aiding

Continue reading…

Performance characterization of HisPur Nickel Superflow Agarose

The study of protein function and structure has been greatly enhanced by recombinant protein expression strategies that incorporate fusions of affinity tags to aid in purification. The poly-histidine fusion tag (His tag) is especially popular because it is small (6-12 amino acids) and provides for simple and gentle purification by immobilized metal affinity chromatography (IMAC),

Continue reading…

Performance characterization of HisPur Cobalt Superflow Agarose

The study of protein function and structure has been greatly enhanced by recombinant protein expression strategies that incorporate fusions of affinity tags to aid in purification. The poly-histidine fusion tag (His tag) is especially popular because it is small (6-12 amino acids) and provides for simple and gentle purification by immobilized metal affinity chromatography (IMAC),

Continue reading…

Previews Volume 14, Issue 1

This 2010 issue of the Previews newsletter features articles about new kits and products for chromatin immunoprecipitation (ChIP assays), Ni-NTA His-tagged protein purification, protein immobilization to NHS-activated agarose, graphite-based phosphopeptide purification, and cell lysis for IP.

Previews was a print magazine that highlighted new Pierce Protein Research products and applications. Articles included information relating to

Continue reading…