Subject Search: Dialysis and Desalting

Benchmarking experiments with Zeba Spin Desalting Columns

Gel filtration chromatography is commonly used for the separation of buffer components from macromolecules, such as proteins and DNA. This application is typically referred to as desalting or buffer exchange. It is an essential technique in life science laboratories, where it is used to remove excess salt, metabolites, biotin, crosslinkers, reactive dyes, radioactive labels and

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Separation characteristics of dialysis membranes

Dialysis is a classic laboratory technique that relies on selective diffusion of molecules across a semi-permeable membrane to separate molecules based on size. Dialysis is used for a wide variety of applications: desalting, buffer exchange, removal of labeling reagents, drug binding studies, cell growth and feeding, virus purification, and blood treatment. Typically, a sample and

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Chromatography cartridges for desalting and affinity purification

A variety of desalting and affinity-purification supports are available pre-packed in 1mL and 5mL Thermo Scientific Pierce Chromatography Cartridges for processing manually or by automated liquid chromatography (LC) systems. The cartridges attach directly to ÄKTA* or FPLC Systems without additional connectors. Cartridge products include an accessory pack (also available separately) of tubing fittings and Luer-Lok*

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Low-volume sample dialysis

The Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices are a convenient and easy means for dialyzing small-volume protein samples (10-100μL). Our new the 0.5mL and 2mL Slide-A-Lyzer* MINI Dialysis Devices can process 50-500μL and 200-2000μL samples, respectively, and are self-contained, single-use devices that require no syringes, centrifuge, beakers or laborious steps. Sample addition and removal are

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