Subject Search: Affinity Purification

Calculate the number of immobilized proteins per bead of agarose affinity supports

Protein biology research as we know it would not be possible without the variety of chromatography resins, beads, membranes, plastic surfaces and other solid or porous materials to immobilize proteins and affinity ligands. Because these tools and methods are just a means to an end rather than the primary focus of research, it is easy

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Performance characterization of Pierce Glutathione Superflow Agarose

The study of protein function and structure has been greatly enhanced by recombinant protein expression strategies that incorporate fusions of affinity tags to aid in purification. The glutathione S-transferase (GST) tag is particularly popular for two primary reasons: (1) the GST tag usually increases the solubility of proteins to which it is fused, thereby aiding

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Performance characterization of HisPur Nickel Superflow Agarose

The study of protein function and structure has been greatly enhanced by recombinant protein expression strategies that incorporate fusions of affinity tags to aid in purification. The poly-histidine fusion tag (His tag) is especially popular because it is small (6-12 amino acids) and provides for simple and gentle purification by immobilized metal affinity chromatography (IMAC),

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Performance characterization of HisPur Cobalt Superflow Agarose

The study of protein function and structure has been greatly enhanced by recombinant protein expression strategies that incorporate fusions of affinity tags to aid in purification. The poly-histidine fusion tag (His tag) is especially popular because it is small (6-12 amino acids) and provides for simple and gentle purification by immobilized metal affinity chromatography (IMAC),

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Chromatography cartridges for desalting and affinity purification

A variety of desalting and affinity-purification supports are available pre-packed in 1mL and 5mL Thermo Scientific Pierce Chromatography Cartridges for processing manually or by automated liquid chromatography (LC) systems. The cartridges attach directly to ÄKTA* or FPLC Systems without additional connectors. Cartridge products include an accessory pack (also available separately) of tubing fittings and Luer-Lok*

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Phosphoprotein enrichment from cell and tissue samples

Reversible protein phosphorylation regulates nearly all intracellular biological events, including signal transduction, protein-protein interactions, protein stability, protein localization, apoptosis and cell-cycle control. Deregulation of protein phosphorylation is a hallmark of numerous human diseases, including cancer and metabolic and immune disorders. However, poorly developed phospho-specific antibodies, the labile state of the phosphate group, and low phosphoprotein

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Antibody purification column formats

Protein A, Protein G, Protein A/G and Protein L are antibody-binding proteins useful for a variety of applications but are typically used while immobilized onto a solid support. Once immobilized, antibody-binding proteins are effective for antibody purification or immunoprecipitation of a target antigen. The particular species and class of antibody to be purified determines which

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Improved immobilization and conjugation of glycoproteins

Aldehyde-hydrazide chemistry has been used for over 30 years for coupling oxidized glycoproteins to activated agarose supports. This chemistry is pH- and time-sensitive, and can result in low yields even after 48 hours of incubation. We have optimized the buffer conditions and added a catalyst to reduce the reaction time while maximizing coupling.

The Thermo

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Protein A/G magnetic beads

The Thermo Scientific Pierce Protein A/G Magnetic Beads enable efficient immunoprecipitation (IP), co-immunoprecipitation (co-IP) and antibody purification with high purity and low background. These unique 1µm particles consist of a polymeric core coated with two layers of magnetite and sealed with a blocking agent to reduce nonspecific binding (Table 1; Figure 1). The bead surface

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GTPase enrichment using a new active-site probe

The Thermo Scientific Pierce GTPase Enrichment Kit with ActivX* GTP Probe enables selective labeling and enrichment of small GTPases and large G-protein subunits. The Thermo Scientific ActivX Desthiobiotin-GTP Probe† structure consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond (Figure 1A). After removal of GTP or GDP nucleotides from enzymes,

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