HeLa cell protein digest standard for mass spectrometry

Proteomics Reagents for Mass Spectrometry

Our new Pierce HeLa Protein Digest Standard is a highly validated tryptic digest of HeLa cell lysate for quality control and system testing in mass spectrometry (MS) analysis of complex proteomic samples.

Continue reading…

When product integrity and risk minimization are paramount

Crosslinking-and-Protein-Modification

Did you notice that we recently introduced a line of Pierce Premium Grade Reagents? These are high-quality formulations of selected chemical modification reagents, specially characterized for applications where product integrity and risk minimization are important.

Continue reading…

Glowing results in one step

Luciferase Reporter Assays

The new Pierce Firefly Luc One-Step Glow Assay Kit expands our growing line of luciferase assay reagents to provide a way to measure firefly luciferase activity in mammalian cell culture using a single reagent-addition step.

Continue reading…

Forget hash tags! Think c-Myc tag IP.

Immunoprecipitation and Pull-Down Kits

New additions to our lines of magnetic beads and agarose resins provide a complete offering of affinity media to immunoprecipitate, co-immunoprecipitate and pull-down c-Myc-tagged proteins and interactors.

Continue reading…

We’ll help you isolate and culture primary neurons, if you’ve got the brains for it!

Product-Articles-Art-250

In a new product article, Hai-Yan Wu and colleagues present results from product validation experiments with the new Thermo Scientific Pierce Primary Neuron Isolation Kit.

Continue reading…

The Video Library is now open. You’re welcome.

Methods-Resources-Art-250

Did you noticed that videos have started popping up around here? From products to methods, they’re showing up all over the place!

Continue reading…

Tandem mass spectrometry for glycan research

Tandem Mass Tag (TMT) Reagents

Our new carbonyl-reactive aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterization and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidized proteins) by mass spectrometry (MS).

Continue reading…

Highly active proteins expressed by IVT

Product-Articles-Art-250

A new product article demonstrates the ability of the Thermo Scientific 1-Step Human High-Yield In Vitro Translation Kit to rapidly express highly functional caspase-3 enzyme, as well as how the system can be used to screen modulators of protein activity.

Continue reading…

Quantitative co-IP without elution?

Co-Immunoprecipitation and Pull-Down Kits

The new Pierce qIP Protein Interaction Kits use anti- HA or anti-Myc beads (agarose or magnetic) and a sensitive luciferase assay system to co-immunoprecipitate (co-IP) and quantify interactions between epitope-tagged and Tluc-tagged protein pairs expressed in mammalian cells.

Continue reading…

In vitro protein expression in a 96-well format

Product-Articles-Art-250

A new product article demonstrates how to use 96-well microdialysis plates to perform high-yield in vitro translation (IVT), also called cell-free protein expression, with Thermo Scientific Human High-Yield Mini IVT Kits.

Continue reading…