Tech Tips for Protein Biology

Tech Tips

Printable reference guides and protocols for protein techniques.

Tech Tips are printable pdf documents that provide protocols or basic reference information of protein biology techniques that are supplementary to product instructions for Thermo Scientific Pierce Protein Biology Products.

Many of these documents have been very popular resources on our website for many years and continue to be so. The guide to Extinction Coefficients (Tech Tip #6) is one such example. Other Tech Tips reflect unusual protocols to illustrate unique experimental strategies and product applications. Certain documents are somewhat outdated but nonetheless serve to illustrate basic laboratory techniques involving proteins.

Use this interactive table to find relevant Tech Tips in our current library.

Doc Number
Tech Tip #1Attach a protein onto glass, silica or quartz surface using a cleavable crosslinkerCrosslinking
Tech Tip #2Attach a protein onto a gold surfaceCrosslinking
Tech Tip #3Determine reactivity of NHS ester biotinylation and crosslinking reagentsCrosslinking, Protein Labeling
Tech Tip #4Batch and spin cup methods for affinity purification of proteinsAffinity Purification
Tech Tip #5Attach an antibody onto glass, silica or quartz surfaceCrosslinking
Tech Tip #6Extinction coefficients and estimation of protein concentration by absorbance at 280nmProtein Assays
Tech Tip #7Remove air bubbles from columns to restore flow rateAffinity Purification
Tech Tip #8Eliminate interfering substances from samples for BCA Protein AssayProtein Assays
Tech Tip #9Quantitate immobilized proteinProtein Assays
Tech Tip #11Light sources and conditions for photoactivation of aryl azide crosslinking reagentsCrosslinking
Tech Tip #12Prepare molecules with poor solubility for immobilization on affinity supportsAffinity Purification
Tech Tip #13Pack gel into affinity columnsAffinity Purification
Tech Tip #14Perform labeling and other reactions in Slide-A-Lyzer Dialysis CassettesProtein Labeling
Tech Tip #15Biotinylate carboxyl groups with EDC and Biotin HydrazideProtein Labeling
Tech Tip #16Block endogenous biotinELISA, IHC, Western Blotting
Tech Tip #18Block amine groups to prevent polymer formation in immunogen productionCrosslinking
Tech Tip #19Remove detergent from protein samplesSample Preparation
Tech Tip #20Dialysis: an overviewSample Preparation
Tech Tip #21Convert to SuperSignal West Pico Substrate from ECL SubstrateWestern Blotting
Tech Tip #22Determine source of nonspecific background signal in a Western blot with chemiluminescent substratesWestern Blotting
Tech Tip #23Strip and reprobe Western blotsWestern Blotting
Tech Tip #24Optimize antigen and antibody concentrations for Western blotsWestern Blotting
Tech Tip #25Determine acceptable wavelengths for measuring protein assaysProtein Assays
Tech Tip #26Prepare a reversible sulfhydryl-reactive affinity columnAffinity Purification
Tech Tip #27Optimize elution conditions for immunoaffinity purificationAffinity Purification
Tech Tip #28Strip Northern and Southern blotsNucleic Acids
Tech Tip #29Degas solutions for use in affinity and gel filtration columnsAffinity Purification
Tech Tip #30Modify and label oligonucleotide 5’-phosphate groupsNucleic Acids
Tech Tip #31Calculate dye:protein (F/P) ratiosProtein Labeling
Tech Tip #32Guide to enzyme substrates for Western blotting applicationsWestern Blotting
Tech Tip #33Guide to enzyme substrates for ELISA applicationsELISA
Tech Tip #34Binding characteristics of Protein A, G, A/G, and L for immunoglobulinsAffinity Purification
Tech Tip #40Convert between times gravity (x g) and rotor speed (RPM)Sample Preparation
Tech Tip #43Protein stability and storageSample Preparation
Tech Tip #45Anneal complementary pairs of oligonucleotidesNucleic Acids
Tech Tip #46Preferentially biotinylate N-terminal a-amino groups in peptidesProtein Labeling
Tech Tip #49Acetone precipitation of proteinsSample Preparation
Tech Tip #50Process stained polyacrylamide gels for mass spectrometryProtein Electrophoresis, Mass Spectrometry
Tech Tip #51Purify proteins from polyacrylamide gelsSample Preparation, Protein Electrophoresis
Tech Tip #52Pierce Spin Cup and Column dimensions and volumesSample Preparation
Tech Tip #53Pierce Concentrator dimensionsSample Preparation
Tech Tip #54Slide-A-Lyzer Dialysis Cassette and MINI Unit dimensionsSample Preparation
Tech Tip #55Remove BSA and gelatin from antibody solutions using Melon GelAffinity Purification
Tech Tip #56Calculate reagent logP values to determine solubility characteristicsSample Preparation
Tech Tip #57How to use a protein assay standard curveProtein Assays
Tech Tip #58Linearity and spike-and-recovery experiments for ELISAELISA
Tech Tip #59Choosing a secondary antibody: A guide to fragment specificityELISA, IHC, Western Blotting
Tech Tip #60Prepare SILAC peptides using the In-Gel Tryptic Digestion KitMass Spectrometry
Tech Tip #61De-paraffinize tissue samples for staining or immunohistochemical proceduresIHC
Tech Tip #62Ion exchange chromatographySample Preparation
Tech Tip #64Immunoprecipitation (IP) technical guide and protocolsAffinity Purification
Tech Tip #65ELISA guideELISA
Tech Tip #67Chemiluminescent Western blotting technical guide and protocolsWestern Blotting
Tech Tip #68Protein assay compatibility tableProtein Assays
Tech Tip #69Removal of unreacted TMT label reagentMass Spectrometry
Tech Tip #70Data acquisition using LC-MS/MS with High Collision Dissociation (HCD) MS/MS of TMT labeled PeptidesMass Spectrometry, HPLC, Liquid Chromatography
Tech Tip #72PCR protocol for generating optimized templates for Pierce Human In Vitro Expression KitsNucleic Acids
Tech Tip #73Electroblot proteins by wet or semi-dry transferProtein Electrophoresis, Western Blotting
Tech Tip #75Measure protein bound to Pierce NHS-Activated Magnetic BeadsProtein Assays
Tech Tip #77Cloning guide for Pierce qIP Protein Interaction KitsAffinity Purification, Protein Expression
Tech Tip #78Prepare and use Slide-A-Lyzer Concentrating Solution (PEG-20 solution): Part No. 66528, 66529, 66530Sample Preparation