Tech Tips
Printable reference guides and protocols for protein techniques.
Tech Tips are printable pdf documents that provide protocols or basic reference information of protein biology techniques that are supplementary to product instructions for Thermo Scientific Pierce Protein Biology Products.
Many of these documents have been very popular resources on our website for many years and continue to be so. The guide to Extinction Coefficients (Tech Tip #6) is one such example. Other Tech Tips reflect unusual protocols to illustrate unique experimental strategies and product applications. Certain documents are somewhat outdated but nonetheless serve to illustrate basic laboratory techniques involving proteins.
Use this interactive table to find relevant Tech Tips in our current library.
| Doc Number | Title | Category |
| Tech Tip #1 | Attach a protein onto glass, silica or quartz surface using a cleavable crosslinker | Crosslinking |
| Tech Tip #2 | Attach a protein onto a gold surface | Crosslinking |
| Tech Tip #3 | Determine reactivity of NHS ester biotinylation and crosslinking reagents | Crosslinking, Protein Labeling |
| Tech Tip #4 | Batch and spin cup methods for affinity purification of proteins | Affinity Purification |
| Tech Tip #5 | Attach an antibody onto glass, silica or quartz surface | Crosslinking |
| Tech Tip #6 | Extinction coefficients and estimation of protein concentration by absorbance at 280nm | Protein Assays |
| Tech Tip #7 | Remove air bubbles from columns to restore flow rate | Affinity Purification |
| Tech Tip #8 | Eliminate interfering substances from samples for BCA Protein Assay | Protein Assays |
| Tech Tip #9 | Quantitate immobilized protein | Protein Assays |
| Tech Tip #11 | Light sources and conditions for photoactivation of aryl azide crosslinking reagents | Crosslinking |
| Tech Tip #12 | Prepare molecules with poor solubility for immobilization on affinity supports | Affinity Purification |
| Tech Tip #13 | Pack gel into affinity columns | Affinity Purification |
| Tech Tip #14 | Perform labeling and other reactions in Slide-A-Lyzer Dialysis Cassettes | Protein Labeling |
| Tech Tip #15 | Biotinylate carboxyl groups with EDC and Biotin Hydrazide | Protein Labeling |
| Tech Tip #16 | Block endogenous biotin | ELISA, IHC, Western Blotting |
| Tech Tip #18 | Block amine groups to prevent polymer formation in immunogen production | Crosslinking |
| Tech Tip #19 | Remove detergent from protein samples | Sample Preparation |
| Tech Tip #20 | Dialysis: an overview | Sample Preparation |
| Tech Tip #21 | Convert to SuperSignal West Pico Subtrate from ECL Substrate | Western Blotting |
| Tech Tip #22 | Determine source of nonspecific background signal in a Western blot with chemiluminescent substrates | Western Blotting |
| Tech Tip #23 | Strip and reprobe Western blots | Western Blotting |
| Tech Tip #24 | Optimize antigen and antibody concentrations for Western blots | Western Blotting |
| Tech Tip #25 | Determine acceptable wavelengths for measuring protein assays | Protein Assays |
| Tech Tip #26 | Prepare a reversible sulfhydryl-reactive affinity column | Affinity Purification |
| Tech Tip #27 | Optimize elution conditions for immunoaffinity purification | Affinity Purification |
| Tech Tip #29 | Degas solutions for use in affinity and gel filtration columns | Affinity Purification |
| Tech Tip #30 | Modify and label oligonucleotide 5-phosphate groups | Nucleic Acids |
| Tech Tip #31 | Calculate dye:protein (F/P) ratios | Protein Labeling |
| Tech Tip #32 | Guide to enzyme substrates for Western blotting applications | Western Blotting |
| Tech Tip #33 | Guide to enzyme substrates for ELISA applications | ELISA |
| Tech Tip #34 | Binding characteristics of Protein A, G, A/G, and L for immunoglobulins | Affinity Purification |
| Tech Tip #40 | Convert between times gravity (x g) and rotor speed (RPM) | Sample Preparation |
| Tech Tip #43 | Protein stability and solubility | Sample Preparation |
| Tech Tip #45 | Anneal complementary pairs of oligonucleotides | Nucleic Acids |
| Tech Tip #46 | Preferentially biotinylate N-terminal a-amino groups in peptides | Protein Labeling |
| Tech Tip #49 | Acetone precipitation of proteins | Sample Preparation |
| Tech Tip #50 | Process stained polyacrylamide gels for mass spectrometry | Protein Electrophoresis, Mass Spectrometry |
| Tech Tip #51 | Purify proteins from polyacrylamide gels | Sample Preparation, Protein Electrophoresis |
| Tech Tip #52 | Pierce Spin Cup and Column dimensions and volumes | Sample Preparation |
| Tech Tip #53 | Pierce Concentrator dimensions | Sample Preparation |
| Tech Tip #54 | Slide-A-Lyzer Dialysis Cassette and MINI Unit dimensions | Sample Preparation |
| Tech Tip #55 | Remove BSA and gelatin from antibody solutions using Melon Gel | Affinity Purification |
| Tech Tip #56 | Calculate reagent logP values to determine solubility characteristics | Sample Preparation |
| Tech Tip #57 | How to use a protein assay standard curve | Protein Assays |
| Tech Tip #58 | Linearity and spike-and-recovery experiments for ELISA | ELISA |
| Tech Tip #59 | Choosing a secondary antibody: A guide to fragment specificity | ELISA, IHC, Western Blotting |
| Tech Tip #60 | Prepare SILAC peptides using the In-Gel Tryptic Digestion Kit | Mass Spectrometry |
| Tech Tip #61 | De-paraffinize tissue samples for staining or immunohistochemical procedures | IHC |
| Tech Tip #62 | Ion exchange chromatography | Sample Preparation |
| Tech Tip #64 | Immunoprecipitation (IP) technical guide and protocols | Affinity Purification |
| Tech Tip #65 | ELISA guide | ELISA |
| Tech Tip #67 | Chemiluminescent Western blotting technical guide and protocols | Western Blotting |
| Tech Tip #68 | Protein assay compatibility table | Protein Assays |
| Tech Tip #69 | Removal of unreacted TMT label reagent | Mass Spectrometry |
| Tech Tip #70 | Data acquisition using LC-MS/MS with High Collision Dissociation (HCD) MS/MS of TMT labeled Peptides | Mass Spectrometry, HPLC, Liquid Chromatography |
| Tech Tip #72 | PCR protocol for generating optimized templates for Pierce Human In Vitro Expression Kits | Nucleic Acids |
| Tech Tip #73 | Electroblot proteins by wet or semi-dry transfer | Protein Electrophoresis, Western Blotting |
| Tech Tip #74 | Quick start guide for Pierce Protocols App for the iPhone Device | Mobile Apps |
| Tech Tip #75 | Measure protein bound to Pierce NHS-Activated Magnetic Beads | Protein Assays |
