Crosslinker Application Guide

Crosslinker Application Guide

Find out how crosslinkers are used in the literature.

Have you ever looked at our crosslinkers and wondered why you would crosslink your own biomolecules? Well, we reviewed the literature to identify recent papers in which the authors describe using our crosslinkers to answer biological questions using the following applications:

  • Interaction analysis – study signal transduction pathways using crosslinkers to trap, isolate and identify interactions between proteins, membrane receptors, peptides and nucleic acids
  • Conjugation – create custom conjugates by covalently linking biomolecules such as proteins, antibodies, enzymes, nucleic acids, drugs, peptides, hormones, metabolites and cellular organelles
  • Immobilization – immobilize antibodies and other proteins, nucleic acids and cells onto resin, extracellular matrix (ECM), chips, slides or nanoparticles
  • in vivo crosslinking – trap protein complexes in live cells by treating cells with crosslinking reagents
  • Labeling – tag proteins or nucleic acids with various reporters, such as fluorophores
  • Chromatin immunoprecipitation (ChIP) – isolate DNA binding complexes by crosslinking and immunoprecipitating DNA binding proteins
  • Structural analysis – study protein oligomerization or the formation of heterologous protein complexes by mapping binding sites
  • Antigen preparation – crosslink antigens to carrier proteins for animal immunization and antibody production
  • Protein alkylation – chemically block amino acid functional groups on proteins

The interactive table below lists the applications, products and references for the papers that we found to give you an idea of the scope of their use and maybe spark your interest in using them to answer your important questions.


Instructions: Click the column heading arrows to sort the table by column
or enter keywords in the field below to filter the table for those keywords.

Application
Product name
Part no.
Reference
Protein-protein interaction analysisMts-Atf-Biotin Label Transfer Reagents33083, 33093Layer G. et al. (2007) SufE transfers sulfur from SufS to SufB for iron-sulfur cluster assembly. J Biol Chem. 282, 13342-13350. PMID: 17350958
Protein-protein interaction analysisMts-Atf-Biotin Label Transfer Reagents33083, 33093Padrick S. B. et al. (2011) Arp2/3 complex is bound and activated by two WASP proteins. Proc Natl Acad Sci U S A. 108, E472-479. PMID: 21676863
Protein-protein interaction analysisMts-Atf-Biotin Label Transfer Reagents33083, 33093Watzlich D. et al. (2009) Chimeric nitrogenase-like enzymes of (bacterio)chlorophyll biosynthesis. J Biol Chem. 284, 15530-15540. PMID: 19336405
Protein-protein interaction analysisL-Photo-Leucine or L-Photo-Methionine22610, 22615, 30030Jumper C. C. and Schriemer D. C. (2011) Mass spectrometry of laser-initiated carbene reactions for protein topographic analysis. Anal Chem. 83, 2913-2920. PMID: 21425771
Protein-membrane protein interaction analysisL-Photo-Leucine or L-Photo-Methionine22610, 22615, 30030Zhao W. Q. et al. (2010) Inhibition of calcineurin-mediated endocytosis and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors prevents amyloid beta oligomer-induced synaptic disruption. J Biol Chem. 285, 7619-7632. PMID: 20032460
Protein-membrane protein interaction analysisL-Photo-Leucine or L-Photo-Methionine22610, 22615, 30030Zhao W. Q. et al. (2010) Inhibition of calcineurin-mediated endocytosis and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors prevents amyloid beta oligomer-induced synaptic disruption. J Biol Chem. 285, 7619-7632. PMID: 20032460
Protein-membrane protein interaction analysisL-Photo-Leucine or L-Photo-Methionine22610, 22615, 30030Weaver M. S. et al. (2008) The copper binding domain of SPARC mediates cell survival in vitro via interaction with integrin beta1 and activation of integrin-linked kinase. J Biol Chem. 283, 22826-22837. PMID: 18503049
Protein-protein interaction analysisSulfo-SBED Biotin 33033, 33034, 33073Emara M. et al. (2012) Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite. J Biol Chem. 287, 5756-5763. PMID: 22205703
Protein-protein interaction analysisSulfo-SBED Biotin 33033, 33034, 33073Emara M. et al. (2012) Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite. J Biol Chem. 287, 5756-5763. PMID: 22205703
Protein-protein interaction analysisSulfo-SBED Biotin 33033, 33034, 33073Loers G. et al. (2012) The interaction between cell adhesion molecule L1, matrix metalloproteinase 14, and adenine nucleotide translocator at the plasma membrane regulates L1-mediated neurite outgrowth of murine cerebellar neurons. J Neurosci. 32, 3917-3930. PMID: 22423112
Protein-membrane protein interaction analysisSulfo-SBED Biotin 33033, 33034, 33073Moore B. et al. (2011) Structural basis of molecular recognition of the Leishmania small hydrophilic endoplasmic reticulum-associated protein (SHERP) at membrane surfaces. J Biol Chem. 286, 9246-9256. PMID: 21106528
Antibody/protein immobilization onto resinDMA20660Peng L. et al. (2012) SIRT1 negatively regulates the activities, functions, and protein levels of hMOF and TIP60. Mol Cell Biol. 32, 2823-2836. PMID: 22586264
Protein-membrane protein interaction analysisDMA20660Fenton R. A. et al. (2007) Cellular and subcellular distribution of the type-2 vasopressin receptor in the kidney. Am J Physiol Renal Physiol. 293, F748-760. PMID: 17553938
Protein oligomerizationDMA20660Korczynska J. E. et al. (2012) The structural characterization of a prophage-encoded extracellular DNase from Streptococcus pyogenes. Nucleic Acids Res. 40, 928-938. PMID: 21948797
Chromatin immunoprecipitation (ChIP)DMA20660Mizutani A. et al. (2011) Cell type-specific target selection by combinatorial binding of Smad2/3 proteins and hepatocyte nuclear factor 4alpha in HepG2 cells. J Biol Chem. 286, 29848-29860. PMID: 21646355
Protein-membrane protein interaction analysisDMP21666, 21667Fenton R. A. et al. (2007) Cellular and subcellular distribution of the type-2 vasopressin receptor in the kidney. Am J Physiol Renal Physiol. 293, F748-760. PMID: 17553938
Antibody/protein immobilization onto resinDMP21666, 21667Guergova-Kuras M. et al. (2011) Discovery of lung cancer biomarkers by profiling the plasma proteome with monoclonal antibody libraries. Mol Cell Proteomics. 10, M111 010298. PMID: 21947365
Protein oligomerizationDMS20700Korczynska J. E. et al. (2012) The structural characterization of a prophage-encoded extracellular DNase from Streptococcus pyogenes. Nucleic Acids Res. 40, 928-938. PMID: 21948797
Protein oligomerizationDMS20700Sen D. et al. (2012) Human mitochondrial DNA helicase TWINKLE is both an unwinding and annealing helicase. J Biol Chem. 287, 14545-14556. PMID: 22383523
Protein-membrane protein interaction analysisDMS20700Chang T. W. et al. (2012) Outer membrane lipoprotein Lpp is Gram-negative bacterial cell surface receptor for cationic antimicrobial peptides. J Biol Chem. 287, 418-428. PMID: 22084237
Peptide-protein interaction analysisDMS20700Chang T. W. et al. (2012) Outer membrane lipoprotein Lpp is Gram-negative bacterial cell surface receptor for cationic antimicrobial peptides. J Biol Chem. 287, 418-428. PMID: 22084237
Antibody/protein immobilization onto resinDMS20700Guergova-Kuras M. et al. (2011) Discovery of lung cancer biomarkers by profiling the plasma proteome with monoclonal antibody libraries. Mol Cell Proteomics. 10, M111 010298. PMID: 21947365
Protein oligomerizationDMS20700Jha B. K. et al. (2011) Inhibition of RNase L and RNA-dependent protein kinase (PKR) by sunitinib impairs antiviral innate immunity. J Biol Chem. 286, 26319-26326. PMID: 21636578
Chromatin immunoprecipitation (ChIP)DTBP20665Zhu B. et al. (2012) Peroxisome proliferator-activated receptor beta/delta cross talks with E2F and attenuates mitosis in HRAS-expressing cells. Mol Cell Biol. 32, 2065-2082. PMID: 22473992
Cell immobilizationDTBP20665Bhattacharya M. et al. (2012) IQGAP1 is necessary for pulmonary vascular barrier protection in murine acute lung injury and pneumonia. Am J Physiol Lung Cell Mol Physiol. 303, L12-19. PMID: 22561460
Protein-protein interaction analysisDTBP20665Shi Y. et al. (2011) Two novel WD40 domain-containing proteins, Ere1 and Ere2, function in the retromer-mediated endosomal recycling pathway. Mol Biol Cell. 22, 4093-4107. PMID: 21880895
Protein immobilization onto chip or slideBS(PEG)5, BS(PEG)921581, 21582Blank K. et al. (2003) A force-based protein biochip. Proc Natl Acad Sci U S A. 100, 11356-11360. PMID: 12975526
Protein immobilization onto chip or slideBS(PEG)5, BS(PEG)921581, 21582Blank K. et al. (2003) A force-based protein biochip. Proc Natl Acad Sci U S A. 100, 11356-11360. PMID: 12975526
Protein oligomerizationBS(PEG)5, BS(PEG)921581, 21582Lay F. T. et al. (2012) Dimerization of plant defensin NaD1 enhances its antifungal activity. J Biol Chem. 287, 19961-19972. PMID: 22511788
Protein-protein interaction analysisBS(PEG)5, BS(PEG)921581, 21582Petrie R. J. et al. (2012) Nonpolarized signaling reveals two distinct modes of 3D cell migration. J Cell Biol. 197, 439-455. PMID: 22547408
Protein oligomerizationBS321580, 21585, 21586Lay F. T. et al. (2012) Dimerization of plant defensin NaD1 enhances its antifungal activity. J Biol Chem. 287, 19961-19972. PMID: 22511788
Protein-protein conjugate formationBS321580, 21585, 21586Kilkenny M. L. et al. (2012) A conserved motif in the C-terminal tail of DNA polymerase alpha tethers primase to the eukaryotic replisome. J Biol Chem. 287, 23740-23747. PMID: 22593576
in vivo protein crosslinkingBS321580, 21585, 21586van Meeteren L. A. et al. (2012) Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting. J Biol Chem. 287, 18551-18561. PMID: 22493445
Protein-protein conjugate formationBS321580, 21585, 21586Richter C. et al. (2012) The tumor necrosis factor receptor stalk regions define responsiveness to soluble versus membrane-bound ligand. Mol Cell Biol. 32, 2515-2529. PMID: 22547679
Protein oligomerizationBS321580, 21585, 21586Sachais B. S. et al. (2012) Rational design and characterization of platelet factor 4 antagonists for the study of heparin-induced thrombocytopenia. Blood. 119, 5955-5962. PMID: 22452981
Matrix formationBS321580, 21585, 21586Ohgo K. et al. (2012) Resolving nitrogen-15 and proton chemical shifts for mobile segments of elastin with two-dimensional NMR spectroscopy. J Biol Chem. 287, 18201-18209. PMID: 22474297
Protein oligomerizationBS321580, 21585, 21586Aryal R. P. et al. (2012) Tight complex formation between Cosmc chaperone and its specific client non-native T-synthase leads to enzyme activity and client-driven dissociation. J Biol Chem. 287, 15317-15329. PMID: 22416136
Protein-membrane protein interaction analysisBS321580, 21585, 21586Ohnishi H. et al. (2012) Identification of mannose receptor as receptor for hepatocyte growth factor beta-chain: novel ligand-receptor pathway for enhancing macrophage phagocytosis. J Biol Chem. 287, 13371-13381. PMID: 22354962
Protein-membrane protein interaction analysisBSOCOES21600Fenton R. A. et al. (2007) Cellular and subcellular distribution of the type-2 vasopressin receptor in the kidney. Am J Physiol Renal Physiol. 293, F748-760. PMID: 17553938
Protein-membrane protein interaction analysisBSOCOES21600Grinberg M. et al. (2005) Mitochondrial carrier homolog 2 is a target of tBID in cells signaled to die by tumor necrosis factor alpha. Mol Cell Biol. 25, 4579-4590. PMID: 15899861
Protein oligomerizationDSG20593Wang Z. et al. (2012) The structural basis for the oligomerization of the N-terminal domain of SATB1. Nucleic Acids Res. 40, 4193-4202. PMID: 22241778
Protein-membrane protein interaction analysisDSG20593Akin B. L. and Jones L. R. (2012) Characterizing phospholamban to sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a) protein binding interactions in human cardiac sarcoplasmic reticulum vesicles using chemical cross-linking. J Biol Chem. 287, 7582-93. PMID: 22247554
Chromatin immunoprecipitation (ChIP)DSG20593Sen N. and Snyder S. H. (2011) Neurotrophin-mediated degradation of histone methyltransferase by S-nitrosylation cascade regulates neuronal differentiation. Proc Natl Acad Sci U S A. 108, 20178-83. PMID: 22123949
Chromatin immunoprecipitation (ChIP)DSG20593Brasier A. R. et al. (2011) RelA Ser276 phosphorylation-coupled Lys310 acetylation controls transcriptional elongation of inflammatory cytokines in respiratory syncytial virus infection. J Virol. 85, 11752-69. PMID: 21900162
Protein oligomerizationDSG20593Strang C. et al. (2001) A central role for the T1 domain in voltage-gated potassium channel formation and function. J Biol Chem. 276, 28493-502. PMID: 11312262
Chromatin immunoprecipitation (ChIP)DSG20593Mardaryev A. N. et al. (2011) Lhx2 differentially regulates Sox9, Tcf4 and Lgr5 in hair follicle stem cells to promote epidermal regeneration after injury. Development. 138, 4843-52. PMID: 22028024
in vivo protein crosslinkingDSP22585, 22586Larimore J. et al. (2011) The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse. Mol Biol Cell. 22, 4854-67. PMID: 21998198
in vivo protein crosslinkingDSP22585, 22586Larimore J. et al. (2011) The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse. Mol Biol Cell. 22, 4854-67. PMID: 21998198
in vivo protein crosslinkingDSP22585, 22586Zlatic S. A. et al. (2011) Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells. Mol Biol Cell. 22, 1699-715. PMID: 21411634
in vivo protein crosslinkingDSP22585, 22586Penmatsa H. et al. (2010) Compartmentalized cyclic adenosine 3',5'-monophosphate at the plasma membrane clusters PDE3A and cystic fibrosis transmembrane conductance regulator into microdomains. Mol Biol Cell. 21, 1097-110. PMID: 20089840
Protein-protein interaction analysisDSP22585, 22586Gavazzi F. et al. (2007) Wild-type opaque2 and defective opaque2 polypeptides form complexes in maize endosperm cells and bind the opaque2-zein target site. Plant Physiol. 145, 933-45. PMID: 17827273
in vivo protein crosslinkingDSP22585, 22586Smith A. L. et al. (2012) Association of Rho-associated protein kinase 1 with E-cadherin complexes is mediated by p120-catenin. Mol Biol Cell. 23, 99-110. PMID: 22031287
in vivo protein crosslinkingDSP22585, 22586Bue C. A. and Barlowe C. (2009) Molecular dissection of Erv26p identifies separable cargo binding and coat protein sorting activities. J Biol Chem. 284, 24049-24060. PMID: 19574229
Protein-membrane protein interaction analysisDSP22585, 22586Steiner H. et al. (2008) Chemical cross-linking provides a model of the gamma-secretase complex subunit architecture and evidence for close proximity of the C-terminal fragment of presenilin with APH-1. J Biol Chem. 283, 34677-34686. PMID: 18801744
Chromatin immunoprecipitation (ChIP)DSP22585, 22586Denver R. J. and Williamson K. E. (2009) Identification of a thyroid hormone response element in the mouse Kruppel-like factor 9 gene to explain its postnatal expression in the brain. Endocrinology. 150, 3935-3943. PMID: 19359381
Protein-protein interaction analysisDSS21555, 21655, 21658Jennebach S. et al. (2012) Crosslinking-MS analysis reveals RNA polymerase I domain architecture and basis of rRNA cleavage. Nucleic Acids Res. 40, 5591-5601. PMID: 22396529
Protein oligomerizationDSS21555, 21655, 21658Ahmad R. et al. (2012) The MUC1-C oncoprotein binds to the BH3 domain of the pro-apoptotic BAX protein and blocks BAX function. J Biol Chem. 287, 20866-20875. PMID: 22544745
in vivo protein crosslinkingDSS21555, 21655, 21658van Meeteren L. A. et al. (2012) Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting. J Biol Chem. 287, 18551-18561. PMID: 22493445
Peptide-protein interaction analysisDSS21555, 21655, 21658Bofill-Cardona E. et al. (2000) Binding of calmodulin to the D2-dopamine receptor reduces receptor signaling by arresting the G protein activation switch. J Biol Chem. 275, 32672-32680. PMID: 10926927
Protein-protein interaction analysisDSS21555, 21655, 21658Yao Y. et al. (2012) Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium. Blood. 119, 5037-5047. PMID: 22474252
Protein oligomerizationDSS21555, 21655, 21658Faye A. et al. (2007) Rat liver carnitine palmitoyltransferase 1 forms an oligomeric complex within the outer mitochondrial membrane. J Biol Chem. 282, 26908-26916. PMID: 17650509
Antibody/protein immobilization onto resinDSS21555, 21655, 21658Sarkar D. K. et al. (2012) Opiate antagonist prevents mu- and delta-opiate receptor dimerization to facilitate ability of agonist to control ethanol-altered natural killer cell functions and mammary tumor growth. J Biol Chem. 287, 16734-16747. PMID: 22451667
Matrix formationDST20589Matsuda M. et al. (2012) Enhanced tissue penetration-induced high bonding strength of a novel tissue adhesive composed of cholesteryl group-modified gelatin and disuccinimidyl tartarate. Colloids Surf B Biointerfaces. 91, 48-56. PMID: 22088758
Protein oligomerizationDST20589Yu Y. et al. (2009) Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Proc Natl Acad Sci U S A. 106, 11558-11563. PMID: 19556541
Protein oligomerizationDST20589Alian A. et al. (2009) Catalytically-active complex of HIV-1 integrase with a viral DNA substrate binds anti-integrase drugs. Proc Natl Acad Sci U S A. 106, 8192-8197. PMID: 19416821
Protein oligomerizationDST20589Nakayama K. et al. (2007) Arabidopsis Cor15am is a chloroplast stromal protein that has cryoprotective activity and forms oligomers. Plant Physiol. 144, 513-523. PMID: 17384167
Protein-protein interaction analysisDTSSP21578Hung C. W. et al. (2012) Adaptor autoregulation promotes coordinated binding within clathrin coats. J Biol Chem. 287, 17398-17407. PMID: 22457357
Protein oligomerizationDTSSP21578Fenyk S. et al. (2012) A nucleotide phosphatase activity in the nucleotide binding domain of an orphan resistance protein from rice. J Biol Chem. 287, 4023-4032. PMID: 22157756
Protein-membrane protein interaction analysisDTSSP21578Boleij A. et al. (2011) Surface-affinity profiling to identify host-pathogen interactions. Infect Immun. 79, 4777-4783. PMID: 21947776
Protein-protein interaction analysisDTSSP21578Li H. and Pauza C. D. (2011) HIV envelope-mediated, CCR5/alpha4beta7-dependent killing of CD4-negative gammadelta T cells which are lost during progression to AIDS. Blood. 118, 5824-5831. PMID: 21926353
Protein-protein interaction analysisDTSSP21578Liu H. et al. (2011) Psb27, a transiently associated protein, binds to the chlorophyll binding protein CP43 in photosystem II assembly intermediates. Proc Natl Acad Sci U S A. 108, 18536-18541. PMID: 22031695
in vivo protein crosslinkingEGS21565Geula S. et al. (2012) Structure-based analysis of VDAC1 protein: defining oligomer contact sites. J Biol Chem. 287, 2179-2190. PMID: 22117062
Chromatin immunoprecipitation (ChIP)EGS21565Mokry M. et al. (2012) Integrated genome-wide analysis of transcription factor occupancy, RNA polymerase II binding and steady-state RNA levels identify differentially regulated functional gene classes. Nucleic Acids Res. 40, 148-158. PMID: 21914722
Protein oligomerizationEGS21565Gogada R. et al. (2011) Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation. J Biol Chem. 286, 28749-28760. PMID: 21712378
Protein oligomerizationEGS21565Keinan N. et al. (2010) Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis. Mol Cell Biol. 30, 5698-5709. PMID: 20937774
Chromatin immunoprecipitation (ChIP)EGS21565Pourcet B. et al. (2011) LXRalpha regulates macrophage arginase 1 through PU.1 and interferon regulatory factor 8. Circ Res. 109, 492-501. PMID: 21757649
Protein oligomerizationSulfo-EGS21566Xayarath B. et al. (2011) A novel C-terminal mutation resulting in constitutive activation of the Listeria monocytogenes central virulence regulatory factor PrfA. Microbiology. 157, 3138-3149. PMID: 21835879
Protein-membrane protein interaction analysisSulfo-EGS21566Moore B. et al. (2011) Structural basis of molecular recognition of the Leishmania small hydrophilic endoplasmic reticulum-associated protein (SHERP) at membrane surfaces. J Biol Chem. 286, 9246-9256. PMID: 21106528
Protein-membrane protein interaction analysisSulfo-EGS21566Ozvegy-Laczka C. et al. (2008) Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter. J Biol Chem. 283, 26059-26070. PMID: 18644784
Protein-membrane protein interaction analysisSulfo-EGS21566Deiss K. et al. (2012) Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2. J Biol Chem. 287, 23407-23417. PMID: 22610096
Protein oligomerizationSulfo-EGS21566Deiss K. et al. (2012) Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2. J Biol Chem. 287, 23407-23417. PMID: 22610096
Protein oligomerizationSulfo-EGS21566Kim J. et al. (2011) Role of TRIM5alpha RING domain E3 ubiquitin ligase activity in capsid disassembly, reverse transcription blockade, and restriction of simian immunodeficiency virus. J Virol. 85, 8116-8132. PMID: 21680520
Protein oligomerizationSulfo-EGS21566Severin S. et al. (2011) Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signaling. J Biol Chem. 286, 4107-4116. PMID: 21098033
Protein oligomerizationSulfo-EGS21566Jansma A. L. et al. (2010) NMR analysis of the structure, dynamics, and unique oligomerization properties of the chemokine CCL27. J Biol Chem. 285, 14424-14437. PMID: 20200157
Peptide-protein interaction analysisTSAT33063Bofill-Cardona E. et al. (2000) Binding of calmodulin to the D2-dopamine receptor reduces receptor signaling by arresting the G protein activation switch. J Biol Chem. 275, 32672-32680. PMID: 10926927
in vivo protein crosslinkingDFDNB21525Geula S. et al. (2012) Structure-based analysis of VDAC1 protein: defining oligomer contact sites. J Biol Chem. 287, 2179-2190. PMID: 22117062
Protein oligomerizationDFDNB21525Geula S. et al. (2012) Structure-based analysis of VDAC1 protein: defining oligomer contact sites. J Biol Chem. 287, 2179-2190. PMID: 22117062
Protein-protein interaction analysisDFDNB21525Schulz C. et al. (2011) Tim50's presequence receptor domain is essential for signal driven transport across the TIM23 complex. J Cell Biol. 195, 643-656. PMID: 22065641
Protein oligomerizationDFDNB21525Sheridan J. T. et al. (2011) Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A. J Biol Chem. 286, 1381-1388. PMID: 21056985
Protein oligomerizationDFDNB21525Keinan N. et al. (2010) Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis. Mol Cell Biol. 30, 5698-5709. PMID: 20937774
Protein-protein interaction analysisDFDNB21525Lucker B. F. et al. (2010) Direct interactions of intraflagellar transport complex B proteins IFT88, IFT52, and IFT46. J Biol Chem. 285, 21508-21518. PMID: 20435895
Protein-protein interaction analysisDFDNB21525Gruschke S. et al. (2010) Proteins at the polypeptide tunnel exit of the yeast mitochondrial ribosome. J Biol Chem. 285, 19022-19028. PMID: 20404317
Protein-small molecule conjugationSIA22349Chen Y. R. et al. (2004) Formation of protein tyrosine ortho-semiquinone radical and nitrotyrosine from cytochrome c-derived tyrosyl radical. J Biol Chem. 279, 18054-18062. PMID: 14761966
Protein oligomerizationSIA22349Strang C. et al. (2001) A central role for the T1 domain in voltage-gated potassium channel formation and function. J Biol Chem. 276, 28493-28502. PMID: 11312262
Protein-protein interaction analysisSIAB22329Stan T. et al. (2003) Mitochondrial protein import: recognition of internal import signals of BCS1 by the TOM complex. Mol Cell Biol. 23, 2239-2250. PMID: 12640110
Nucleic acid immobilizationSIAB22329Andreadis J. D. and Chrisey L. A. (2000) Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions. Nucleic Acids Res. 28, e5. PMID: 10606673
Nucleic acid immobilizationSIAB22329Tang K. et al. (1999) Chip-based genotyping by mass spectrometry. Proc Natl Acad Sci U S A. 96, 10016-10020. PMID: 10468554
Peptide-protein interaction analysisSIAB22329Weng G. et al. (1996) Gbeta subunit interacts with a peptide encoding region 956-982 of adenylyl cyclase 2. Cross-linking of the peptide to free Gbetagamma but not the heterotrimer. J Biol Chem. 271, 26445-26448. PMID: 8900107
Nucleic acid immobilizationSIAB22329Chrisey L. A. et al. (1996) Covalent attachment of synthetic DNA to self-assembled monolayer films. Nucleic Acids Res. 24, 3031-3039. PMID: 8760890
Antibody/protein immobilization onto resinSulfo-SIAB22327Harma H. et al. (2000) Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments. Clin Chem. 46, 1755-1761. PMID: 11067810
Nucleic acid immobilizationSulfo-SIAB22327Adessi C. et al. (2000) Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. 28, E87. PMID: 11024189
Nucleic acid immobilizationSulfo-SIAB22327Allerson C. R. et al. (2003) A high-capacity RNA affinity column for the purification of human IRP1 and IRP2 overexpressed in Pichia pastoris. RNA. 9, 364-374. PMID: 12592010
Nucleic acid immobilizationSulfo-SIAB22327Crawford J. B. and Patton J. G. (2006) Activation of alpha-tropomyosin exon 2 is regulated by the SR protein 9G8 and heterogeneous nuclear ribonucleoproteins H and F. Mol Cell Biol. 26, 8791-8802. PMID: 17000773
Protein-protein interaction analysisAMAS22295Pech M. et al. (2010) Dual binding mode of the nascent polypeptide-associated complex reveals a novel universal adapter site on the ribosome. J Biol Chem. 285, 19679-19687. PMID: 20410297
Protein oligomerizationAMAS22295Tran H. J. et al. (2005) Analysis of RovA, a transcriptional regulator of Yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation. J Biol Chem. 280, 42423-42432. PMID: 16257976
Protein oligomerizationAMAS22295Strang C. et al. (2001) A central role for the T1 domain in voltage-gated potassium channel formation and function. J Biol Chem. 276, 28493-28502. PMID: 11312262
Protein-protein interaction analysisAMAS22295Chen Z. et al. (2003) Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents. J Biol Chem. 278, 48348-48356. PMID: 12972413
Protein-protein interaction analysisBMPS22298Chen Z. et al. (2003) Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents. J Biol Chem. 278, 48348-48356. PMID: 12972413
Protein-protein interaction analysisEMCS22308Chen Z. et al. (2003) Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents. J Biol Chem. 278, 48348-48356. PMID: 12972413
Protein immobilization onto chip or slideEMCS22308Brandt O. et al. (2003) PNA microarrays for hybridisation of unlabelled DNA samples. Nucleic Acids Res. 31, e119. PMID: 14500847
Protein-protein interaction analysisEMCS22308Chen Z. et al. (2005) Role of leucine 31 of phospholamban in structural and functional interactions with the Ca2+ pump of cardiac sarcoplasmic reticulum. J Biol Chem. 280, 10530-10539. PMID: 15644311
Protein-protein interaction analysisEMCS22308Akin B. L. and Jones L. R. (2012) Characterizing phospholamban to sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a) protein binding interactions in human cardiac sarcoplasmic reticulum vesicles using chemical cross-linking. J Biol Chem. 287, 7582-7593. PMID: 22247554
Protein immobilization onto chip or slideEMCS22308Hamby C. V. et al. (2005) Use of Peptide library screening to detect a previously unknown linear diagnostic epitope: proof of principle by use of lyme disease sera. Clin Diagn Lab Immunol. 12, 801-807. PMID: 16002626
Antibody/protein immobilization onto resinEMCS22308Harma H. et al. (2000) Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments. Clin Chem. 46, 1755-1761. PMID: 11067810
Protein-protein interaction analysisEMCS22308Chen Z. et al. (2007) Mechanism of reversal of phospholamban inhibition of the cardiac Ca2+-ATPase by protein kinase A and by anti-phospholamban monoclonal antibody 2D12. J Biol Chem. 282, 20968-20976. PMID: 17548345
Antigen formation for immunizationSulfo-EMCS22307Anderson J. P. et al. (2006) Phosphorylation of Ser-129 is the dominant pathological modification of alpha-synuclein in familial and sporadic Lewy body disease. J Biol Chem. 281, 29739-29752. PMID: 16847063
Nucleic acid immobilizationSulfo-EMCS22307Fixe F. et al. (2004) An on-chip thin film photodetector for the quantification of DNA probes and targets in microarrays. Nucleic Acids Res. 32, e70. PMID: 15148343
Nucleic acid immobilizationSulfo-EMCS22307Fixe F. et al. (2004) Functionalization of poly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays. Nucleic Acids Res. 32, e9. PMID: 14718554
Antigen formation for immunizationGMBS22309Fujiwara K. et al. (1998) Preparation of monoclonal antibodies against N-(gamma-maleimidobutyryloxy)succinimide (GMBS)-conjugated acetylspermine, and development of an enzyme-linked immunosorbent assay (ELISA) for N1,N12-diacetylspermine. J Biochem. 124, 244-249. PMID: 9644270
Nucleic acid immobilizationGMBS22309Adessi C. et al. (2000) Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. 28, E87. PMID: 11024189
Nucleic acid immobilizationGMBS22309Chrisey L. A. et al. (1996) Covalent attachment of synthetic DNA to self-assembled monolayer films. Nucleic Acids Res. 24, 3031-3039. PMID: 8760890
Antigen formation for immunizationGMBS22309Lee S. J. et al. (2011) Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liver. Blood. 117, 5215-5223. PMID: 21427291
Antibody/protein immobilization onto resinGMBS22309Gunning A. P. et al. (2009) Recognition of galactan components of pectin by galectin-3. FASEB J. 23, 415-424. PMID: 18832596
Antibody/protein immobilization onto resinGMBS22309Gunning A. P. et al. (2008) Mapping specific adhesive interactions on living human intestinal epithelial cells with atomic force microscopy. FASEB J. 22, 2331-2339. PMID: 18263697
Antigen formation for immunizationGMBS22309Lee H. S. et al. (2010) Obox4 critically regulates cAMP-dependent meiotic arrest and MI-MII transition in oocytes. FASEB J. 24, 2314-2324. PMID: 20154267
Protein-nucleic acid interaction analysisSulfo-GMBS22324Sheridan J. T. et al. (2011) Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A. J Biol Chem. 286, 1381-1388. PMID: 21056985
Nucleic acid immobilizationSulfo-GMBS22324Adessi C. et al. (2000) Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. 28, E87. PMID: 11024189
Protein oligomerizationSulfo-GMBS22324Faye A. et al. (2007) Rat liver carnitine palmitoyltransferase 1 forms an oligomeric complex within the outer mitochondrial membrane. J Biol Chem. 282, 26908-26916. PMID: 17650509
Protein-membrane protein interaction analysisSulfo-GMBS22324Weber A. N. et al. (2005) Ligand-receptor and receptor-receptor interactions act in concert to activate signaling in the Drosophila toll pathway. J Biol Chem. 280, 22793-22799. PMID: 15795223
Protein immobilization onto chip or slideSulfo-GMBS22324Liu S. H. et al. (2006) Studying the protein organization of the postsynaptic density by a novel solid phase- and chemical cross-linking-based technology. Mol Cell Proteomics. 5, 1019-1032. PMID: 16501281
Nucleic acid immobilizationLC-SMCC22362Masuko M. (2003) Hybridization of an immobilized PNA probe with its complementary oligodeoxyribonucleotide on the surface of silica glass. Nucleic Acids Res Suppl, 145-146. PMID: 14510422
Protein-protein interaction analysisLC-SMCC22362Nguyen M. et al. (2007) Small molecule obatoclax (GX15-070) antagonizes MCL-1 and overcomes MCL-1-mediated resistance to apoptosis. Proc Natl Acad Sci U S A. 104, 19512-19517. PMID: 18040043
Peptide-protein interaction analysisLC-SMCC22362Ruffolo S. C. and Shore G. C. (2003) BCL-2 selectively interacts with the BID-induced open conformer of BAK, inhibiting BAK auto-oligomerization. J Biol Chem. 278, 25039-25045. PMID: 12721291
Nucleic acid immobilizationMBS22311Adessi C. et al. (2000) Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. 28, E87. PMID: 11024189
Nucleic acid immobilizationMBS22311Chrisey L. A. et al. (1996) Covalent attachment of synthetic DNA to self-assembled monolayer films. Nucleic Acids Res. 24, 3031-3039. PMID: 8760890
Protein-protein interaction analysisMBS22311Chen Z. et al. (2003) Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents. J Biol Chem. 278, 48348-48356. PMID: 12972413
Antibody-drug conjugationSMCC22360Kovtun Y. V. et al. (2010) Antibody-maytansinoid conjugates designed to bypass multidrug resistance. Cancer Res. 70, 2528-2537. PMID: 20197459
Nucleic acid immobilizationSMCC22360Chrisey L. A. et al. (1996) Covalent attachment of synthetic DNA to self-assembled monolayer films. Nucleic Acids Res. 24, 3031-3039. PMID: 8760890
Protein-protein interaction analysisSMCC22360Chen Z. et al. (2003) Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents. J Biol Chem. 278, 48348-48356. PMID: 12972413
Nucleic acid immobilizationSMCC22360Adessi C. et al. (2000) Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. 28, E87. PMID: 11024189
Peptide-drug conjugationSMCC22360Hatakeyama S. et al. (2011) Targeted drug delivery to tumor vasculature by a carbohydrate mimetic peptide. Proc Natl Acad Sci U S A. 108, 19587-19592. PMID: 22114188
Inorganic particle conjugationSMCC22360Sperling R. A. and Parak W. J. (2010) Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles. Philos Transact A Math Phys Eng Sci. 368, 1333-1383. PMID: 20156828
Protein-nucleic acid conjugationSMCC22360Obara K. et al. (2001) Protein/oligonucleotide conjugates as a cell specific PNA carrier. Nucleic Acids Res Suppl, 217-218. PMID: 12836342
Protein-protein conjugate formationSulfo-SMCC22122, 22322, 22622Harvey S. et al. (1998) Chondrex: new marker of joint disease. Clin Chem. 44, 509-516. PMID: 9510855
Nucleic acid immobilizationSulfo-SMCC22122, 22322, 22622Adessi C. et al. (2000) Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. 28, E87. PMID: 11024189
Antigen formation for immunizationSulfo-SMCC22122, 22322, 22622Betting D. J. et al. (2008) Sulfhydryl-based tumor antigen-carrier protein conjugates stimulate superior antitumor immunity against B cell lymphomas. J Immunol. 181, 4131-4140. PMID: 18768870
Protein-membrane protein interaction analysisSulfo-SMCC22122, 22322, 22622Mamedova A. A. et al. (2004) Substrate-induced conformational change in bacterial complex I. J Biol Chem. 279, 23830-23836. PMID: 15037611
Protein-protein conjugate formationSulfo-SMCC22122, 22322, 22622Percy A. J. et al. (1996) Development of sensitive immunoassays to detect amylin and amylin-like peptides in unextracted plasma. Clin Chem. 42, 576-585. PMID: 8605675
Protein-nucleic acid conjugationSMPB22416Meng F. and Sachs F. (2011) Visualizing dynamic cytoplasmic forces with a compliance-matched FRET sensor. J Cell Sci. 124, 261-269. PMID: 21172803
Protein-protein conjugate formationSMPB22416Karumuthil-Melethil S. et al. (2010) Dendritic cell-directed CTLA-4 engagement during pancreatic beta cell antigen presentation delays type 1 diabetes. J Immunol. 184, 6695-6708. PMID: 20483724
Nucleic acid immobilizationSMPB22416Chrisey L. A. et al. (1996) Covalent attachment of synthetic DNA to self-assembled monolayer films. Nucleic Acids Res. 24, 3031-3039. PMID: 8760890
in vivo protein crosslinkingSMPB22416Goldoni S. et al. (2009) Decorin is a novel antagonistic ligand of the Met receptor. J Cell Biol. 185, 743-754. PMID: 19433454
Protein-membrane protein interaction analysisSulfo-SMPB22317Zhu J. X. et al. (2005) Decorin evokes protracted internalization and degradation of the epidermal growth factor receptor via caveolar endocytosis. J Biol Chem. 280, 32468-32479. PMID: 15994311
Protein-membrane protein interaction analysisSulfo-SMPB22317Mamedova A. A. et al. (2004) Substrate-induced conformational change in bacterial complex I. J Biol Chem. 279, 23830-23836. PMID: 15037611
Protein oligomerizationSulfo-SMPB22317Goldoni S. et al. (2004) Biologically active decorin is a monomer in solution. J Biol Chem. 279, 6606-6612. PMID: 14660661
Protein-nucleic acid conjugationSulfo-SMPB22317Niemeyer C. M. et al. (2003) Combination of DNA-directed immobilization and immuno-PCR: very sensitive antigen detection by means of self-assembled DNA-protein conjugates. Nucleic Acids Res. 31, e90. PMID: 12907742
Antibody/protein immobilization onto resinSulfo-SMPB22317Pu W. T. et al. (1999) pICln inhibits snRNP biogenesis by binding core spliceosomal proteins. Mol Cell Biol. 19, 4113-4120. PMID: 10330151
Protein-protein interaction analysisSMPH22363Chen Z. et al. (2003) Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents. J Biol Chem. 278, 48348-48356. PMID: 12972413
Protein-protein conjugate formationSMPH22363Schmitz N. et al. (2009) Displaying Fel d1 on virus-like particles prevents reactogenicity despite greatly enhanced immunogenicity: a novel therapy for cat allergy. J Exp Med. 206, 1941-1955. PMID: 19667059
Protein-protein interaction analysisKMUSChen Z. et al. (2007) Mechanism of reversal of phospholamban inhibition of the cardiac Ca2+-ATPase by protein kinase A and by anti-phospholamban monoclonal antibody 2D12. J Biol Chem. 282, 20968-20976. PMID: 17548345
Protein-protein interaction analysisKMUSChen Z. et al. (2003) Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents. J Biol Chem. 278, 48348-48356. PMID: 12972413
Protein-membrane protein interaction analysisKMUSAkin B. L. et al. (2010) Superinhibitory phospholamban mutants compete with Ca2+ for binding to SERCA2a by stabilizing a unique nucleotide-dependent conformational state. J Biol Chem. 285, 28540-28552. PMID: 20622261
Protein-membrane protein interaction analysisKMUSChen Z. et al. (2005) Role of leucine 31 of phospholamban in structural and functional interactions with the Ca2+ pump of cardiac sarcoplasmic reticulum. J Biol Chem. 280, 10530-10539. PMID: 15644311
Protein-membrane protein interaction analysisKMUSChen Z. et al. (2010) Ca2+ binding to site I of the cardiac Ca2+ pump is sufficient to dissociate phospholamban. J Biol Chem. 285, 3253-3260. PMID: 19948724
Antibody-liposome conjugationSulfo-KMUS21111DeCory T. R. et al. (2005) Development of an immunomagnetic bead-immunoliposome fluorescence assay for rapid detection of Escherichia coli O157:H7 in aqueous samples and comparison of the assay with a standard microbiological method. Appl Environ Microbiol. 71, 1856-1864. PMID: 15812012
Protein oligomerizationSulfo-KMUS21111Faye A. et al. (2007) Rat liver carnitine palmitoyltransferase 1 forms an oligomeric complex within the outer mitochondrial membrane. J Biol Chem. 282, 26908-26916. PMID: 17650509
Protein-membrane protein interaction analysisSulfo-KMUS21111Weber A. N. et al. (2005) Ligand-receptor and receptor-receptor interactions act in concert to activate signaling in the Drosophila toll pathway. J Biol Chem. 280, 22793-22799. PMID: 15795223
Nucleic acid immobilizationSulfo-MBS22312Adessi C. et al. (2000) Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. 28, E87. PMID: 11024189
Protein-membrane protein interaction analysisSulfo-MBS22312Ozvegy-Laczka C. et al. (2008) Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter. J Biol Chem. 283, 26059-26070. PMID: 18644784
Protein oligomerizationSulfo-MBS22312Faye A. et al. (2007) Rat liver carnitine palmitoyltransferase 1 forms an oligomeric complex within the outer mitochondrial membrane. J Biol Chem. 282, 26908-26916. PMID: 17650509
Protein-protein conjugate formationSPDP21857Karumuthil-Melethil S. et al. (2010) Dendritic cell-directed CTLA-4 engagement during pancreatic beta cell antigen presentation delays type 1 diabetes. J Immunol. 184, 6695-6708. PMID: 20483724
Protein-protein conjugate formationSPDP21857You H. et al. (2012) Winding single-molecule double-stranded DNA on a nanometer-sized reel. Nucleic Acids Res. PMID: 22772992
Protein-protein conjugate formationSPDP21857Pallaoro A. et al. (2011) Quantitative ratiometric discrimination between noncancerous and cancerous prostate cells based on neuropilin-1 overexpression. Proc Natl Acad Sci U S A. 108, 16559-16564. PMID: 21930955
Protein-membrane protein interaction analysisSPDP21857Lobedanz S. et al. (2007) A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps. Proc Natl Acad Sci U S A. 104, 4612-4617. PMID: 17360572
Protein-protein interaction analysisSPDP21857Janganan T. K. et al. (2011) Evidence for the assembly of a bacterial tripartite multidrug pump with a stoichiometry of 3:6:3. J Biol Chem. 286, 26900-26912. PMID: 21610073
Antibody/protein immobilization onto resinSPDP21857Zhou H. et al. (2011) Development and characterization of a potent immunoconjugate targeting the Fn14 receptor on solid tumor cells. Mol Cancer Ther. 10, 1276-1288. PMID: 21586630
Protein-protein interaction analysisLC-SPDP21651Janganan T. K. et al. (2011) Evidence for the assembly of a bacterial tripartite multidrug pump with a stoichiometry of 3:6:3. J Biol Chem. 286, 26900-26912. PMID: 21610073
Protein-membrane protein interaction analysisLC-SPDP21651Symmons M. F. et al. (2009) The assembled structure of a complete tripartite bacterial multidrug efflux pump. Proc Natl Acad Sci U S A. 106, 7173-7178. PMID: 19342493
Antibody/protein immobilization onto resinLC-SPDP21651Yuan J. and Berg H. C. (2008) Resurrection of the flagellar rotary motor near zero load. Proc Natl Acad Sci U S A. 105, 1182-1185. PMID: 18202173
Protein-membrane protein interaction analysisLC-SPDP21651Lobedanz S. et al. (2007) A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps. Proc Natl Acad Sci U S A. 104, 4612-4617. PMID: 17360572
Protein immobilization onto chip or slideSulfo-LC-SPDP21650Zhao D. et al. (2011) Carbon nanotubes enhance CpG uptake and potentiate antiglioma immunity. Clin Cancer Res. 17, 771-782. PMID: 21088258
Antibody/protein immobilization onto resinSulfo-LC-SPDP21650Zhang J. et al. (2008) Identification of CKAP4/p63 as a major substrate of the palmitoyl acyltransferase DHHC2, a putative tumor suppressor, using a novel proteomics method. Mol Cell Proteomics. 7, 1378-1388. PMID: 18296695
Protein labelingSulfo-LC-SPDP21650Backer M. V. et al. (2005) Vascular endothelial growth factor selectively targets boronated dendrimers to tumor vasculature. Mol Cancer Ther. 4, 1423-1429. PMID: 16170035
Protein-small molecule conjugationSMPT21558Austin C. D. et al. (2005) Oxidizing potential of endosomes and lysosomes limits intracellular cleavage of disulfide-based antibody-drug conjugates. Proc Natl Acad Sci U S A. 102, 17987-17992. PMID: 16322102
Protein-protein conjugate formationSMPT21558Solomon S. R. et al. (2005) Selective depletion of alloreactive donor lymphocytes: a novel method to reduce the severity of graft-versus-host disease in older patients undergoing matched sibling donor stem cell transplantation. Blood. 106, 1123-1129. PMID: 15817673
Protein-small molecule conjugationSMPT21558Windt W. A. et al. (2004) Renal targeting of captopril using captopril-lysozyme conjugate enhances its antiproteinuric effect in adriamycin-induced nephrosis. J Renin Angiotensin Aldosterone Syst. 5, 197-202. PMID: 15803438
Protein-protein conjugate formationSMPT21558Ding B. S. et al. (2003) Lung endothelium targeting for pulmonary embolism thrombolysis. Circulation. 108, 2892-2898. PMID: 14610017
Protein-protein conjugate formationANB-NOS21451Koulov A. V. et al. (2010) Biological and structural basis for Aha1 regulation of Hsp90 ATPase activity in maintaining proteostasis in the human disease cystic fibrosis. Mol Biol Cell. 21, 871-884. PMID: 20089831
Protein-protein interaction analysisANB-NOS21451Gorna M. W. et al. (2010) The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome. RNA. 16, 553-562. PMID: 20106955
Protein-protein interaction analysisANB-NOS21451Adams C. A. et al. (2004) Self-association of the amino-terminal domain of the yeast TATA-binding protein. J Biol Chem. 279, 1376-1382. PMID: 14534318
Protein-protein conjugate formationANB-NOS21451Park B. and Ahn K. (2003) An essential function of tapasin in quality control of HLA-G molecules. J Biol Chem. 278, 14337-14345. PMID: 12582157
Antibody/protein immobilization onto
extracelular matrix
Sulfo-SANPAH22589Oakes P. W. et al. (2012) Tension is required but not sufficient for focal adhesion maturation without a stress fiber template. J Cell Biol. 196, 363-374. PMID: 22291038
Antibody/protein immobilization onto
extracelular matrix
Sulfo-SANPAH22589Elliott C. G. et al. (2012) Periostin modulates myofibroblast differentiation during full-thickness cutaneous wound repair. J Cell Sci. 125, 121-132. PMID: 22266908
Antibody/protein immobilization onto
extracelular matrix
Sulfo-SANPAH22589Mierke C. T. et al. (2011) Contractile forces contribute to increased glycosylphosphatidylinositol-anchored receptor CD24-facilitated cancer cell invasion. J Biol Chem. 286, 34858-34871. PMID: 21828044
Antibody/protein immobilization onto
extracelular matrix
Sulfo-SANPAH22589Liu Z. et al. (2011) Alpha-actinin-4 and CLP36 protein deficiencies contribute to podocyte defects in multiple human glomerulopathies. J Biol Chem. 286, 30795-30805. PMID: 21680739
Antibody/protein immobilization onto
extracelular matrix
Sulfo-SANPAH22589Betz T. et al. (2011) Growth cones as soft and weak force generators. Proc Natl Acad Sci U S A. 108, 13420-13425. PMID: 21813757
Inorganic particle conjugationDCC20320Harney A. S. et al. (2009) Targeted inhibition of Snail family zinc finger transcription factors by oligonucleotide-Co(III) Schiff base conjugate. Proc Natl Acad Sci U S A. 106, 13667-13672. PMID: 19666616
Hormone-drug conjugationDCC20320Sundaram S. et al. (2009) Luteinizing hormone-releasing hormone receptor-targeted deslorelin-docetaxel conjugate enhances efficacy of docetaxel in prostate cancer therapy. Mol Cancer Ther. 8, 1655-1665. PMID: 19509261
Inorganic particle conjugationDCC20320Song E. Q. et al. (2009) Tumor cell targeting using folate-conjugated fluorescent quantum dots and receptor-mediated endocytosis. Clin Chem. 55, 955-963. PMID: 19282359
Small molecule-micelle conjugationDCC20320Chen H. et al. (2008) Release of hydrophobic molecules from polymer micelles into cell membranes revealed by Forster resonance energy transfer imaging. Proc Natl Acad Sci U S A. 105, 6596-6601. PMID: 18445654
Inorganic particle conjugationEDC77149, 22980, 22981Sperling R. A. and Parak W. J. (2010) Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles. Philos Transact A Math Phys Eng Sci. 368, 1333-1383. PMID: 20156828
Carbohydrate-metabolite conjugationEDC77149, 22980, 22981Bergfeld A. K. et al. (2012) Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups: ELUCIDATING THE INTRACELLULAR FATE OF THE NON-HUMAN SIALIC ACID N-GLYCOLYLNEURAMINIC ACID. J Biol Chem. 287, 28865-28881. PMID: 22692205
Nucleic acid labelingEDC77149, 22980, 22981Overath T. et al. (2012) Mapping of O-GlcNAc sites of 20 S proteasome subunits and Hsp90 by a novel biotin-cystamine tag. Mol Cell Proteomics. 11, 467-477. PMID: 22556278
Antibody/protein immobilization onto resinEDC77149, 22980, 22981Olsson N. et al. (2012) Quantitative proteomics targeting classes of motif-containing peptides using immunoaffinity-based mass spectrometry. Mol Cell Proteomics. 11, 342-354. PMID: 22543061
Nucleic acid immobilizationEDC77149, 22980, 22981Adessi C. et al. (2000) Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. 28, E87. PMID: 11024189
Nucleic acid immobilizationEDC77149, 22980, 22981Li Z. et al. (2012) Extensive terminal and asymmetric processing of small RNAs from rRNAs, snoRNAs, snRNAs, and tRNAs. Nucleic Acids Res. 40, 6787-6799. PMID: 22492706
Protein-membrane protein interaction analysisEDC77149, 22980, 22981Ido K. et al. (2012) The conserved His-144 in the PsbP protein is important for the interaction between the PsbP N-terminus and the Cyt b559 subunit of photosystem II. J Biol Chem. 287, 26377-26387. PMID: 22707728
Inorganic particle conjugationSulfo-NHS24510, 24520, 24525Sperling R. A. and Parak W. J. (2010) Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles. Philos Transact A Math Phys Eng Sci. 368, 1333-1383. PMID: 20156828
Carbohydrate-metabolite conjugationSulfo-NHS24510, 24520, 24525Bergfeld A. K. et al. (2012) Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups: ELUCIDATING THE INTRACELLULAR FATE OF THE NON-HUMAN SIALIC ACID N-GLYCOLYLNEURAMINIC ACID. J Biol Chem. 287, 28865-28881. PMID: 22692205
Protein-membrane protein interaction analysisSulfo-NHS24510, 24520, 24525Ido K. et al. (2012) The conserved His-144 in the PsbP protein is important for the interaction between the PsbP N-terminus and the Cyt b559 subunit of photosystem II. J Biol Chem. 287, 26377-26387. PMID: 22707728
Protein immobilization onto chip or slideSulfo-NHS24510, 24520, 24525Guha Thakurta S. et al. (2012) Investigation of platelet responses and clotting characteristics of in situ albumin binding surfaces. J Biomater Appl. 26, 529-547. PMID: 20819918
Protein immobilization onto chip or slideSulfo-NHS24510, 24520, 24525Leysath C. E. et al. (2011) Mouse monoclonal antibodies to anthrax edema factor protect against infection. Infect Immun. 79, 4609-4616. PMID: 21911463
Protein immobilization onto chip or slideNHS25400Yoon S. I. et al. (2012) Epstein-Barr virus IL-10 engages IL-10R1 by a two-step mechanism leading to altered signaling properties. J Biol Chem. 287, 26586-26595. PMID: 22692218
Protein immobilization onto chip or slideNHS25400Ding J. et al. (2012) Structural insights into the Pseudomonas aeruginosa type VI virulence effector Tse1 bacteriolysis and self-protection mechanisms. J Biol Chem. 287, 26911-26920. PMID: 22700987
Protein immobilization onto chip or slideNHS25400Matsumura H. et al. (2012) Crystal structure of the N-terminal domain of the yeast general corepressor Tup1p and its functional implications. J Biol Chem. 287, 26528-26538. PMID: 22707714
Protein immobilization onto chip or slideNHS25400Noborn F. et al. (2012) Heparan sulfate dissociates serum amyloid A (SAA) from acute-phase high-density lipoprotein, promoting SAA aggregation. J Biol Chem. 287, 25669-25677. PMID: 22654109
Antibody/protein immobilization onto resinNHS25400Olsson N. et al. (2012) Quantitative proteomics targeting classes of motif-containing peptides using immunoaffinity-based mass spectrometry. Mol Cell Proteomics. 11, 342-354. PMID: 22543061
Protein immobilization onto chip or slideNHS25400Wollein Waldetoft K. et al. (2012) Streptococcal surface proteins activate the contact system and control its antibacterial activity. J Biol Chem. 287, 25010-25018. PMID: 22648411
Protein immobilization onto chip or slideNHS25400Kim D. K. et al. (2012) Orphan nuclear receptor estrogen-related receptor gamma (ERRgamma) is key regulator of hepatic gluconeogenesis. J Biol Chem. 287, 21628-21639. PMID: 22549789
Protein-membrane protein interaction analysisBMPH22297Ozvegy-Laczka C. et al. (2008) Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter. J Biol Chem. 283, 26059-26070. PMID: 18644784
Protein oligomerizationBMPH22297Ishmael S. S. et al. (2006) Protease domain glycans affect oligomerization, disulfide bond formation, and stability of the meprin A metalloprotease homo-oligomer. J Biol Chem. 281, 37404-37415. PMID: 17040911
Protein-protein conjugate formationEMCH22106Joyce J. et al. (2006) Immunogenicity and protective efficacy of Bacillus anthracis poly-gamma-D-glutamic acid capsule covalently coupled to a protein carrier using a novel triazine-based conjugation strategy. J Biol Chem. 281, 4831-4843. PMID: 16293624
Protein labelingEMCH22106Bao X. et al. (2007) Change in permeant size selectivity by phosphorylation of connexin 43 gap-junctional hemichannels by PKC. Proc Natl Acad Sci U S A. 104, 4919-4924. PMID: 17360407
Protein alkylationEMCH22106Ouertatani-Sakouhi H. et al. (2010) Identification and characterization of novel classes of macrophage migration inhibitory factor (MIF) inhibitors with distinct mechanisms of action. J Biol Chem. 285, 26581-26598. PMID: 20516071
Protein labeling (dendrimer)KMUH22111Yang W. et al. (2006) Molecular targeting and treatment of EGFRvIII-positive gliomas using boronated monoclonal antibody L8A4. Clin Cancer Res. 12, 3792-3802. PMID: 16778107
Small molecule-dendrimer conjugationKMUH22111Wu G. et al. (2006) Targeted delivery of methotrexate to epidermal growth factor receptor-positive brain tumors by means of cetuximab (IMC-C225) dendrimer bioconjugates. Mol Cancer Ther. 5, 52-59. PMID: 16432162
Protein labeling (dendrimer)KMUH22111Yang W. et al. (2006) Molecular targeting and treatment of EGFRvIII-positive gliomas using boronated monoclonal antibody L8A4. Clin Cancer Res. 12, 3792-3802. PMID: 16778107
Protein-protein conjugate formationMPBH22305Chamow S. M. et al. (1992) Conjugation of soluble CD4 without loss of biological activity via a novel carbohydrate-directed cross-linking reagent. J Biol Chem. 267, 15916-15922. PMID: 1639820
Antibody/protein immobilization onto resinMPBH22305Gunning A. P. et al. (2009) Recognition of galactan components of pectin by galectin-3. FASEB J. 23, 415-424. PMID: 18832596
Protein immobilization onto chip or slideMPBH22305Gunning A. P. et al. (2008) Mapping specific adhesive interactions on living human intestinal epithelial cells with atomic force microscopy. FASEB J. 22, 2331-2339. PMID: 18263697
Carbohydrate-metabolite conjugationPDPH22301Atkinson S. F. et al. (2001) Conjugation of folate via gelonin carbohydrate residues retains ribosomal-inactivating properties of the toxin and permits targeting to folate receptor positive cells. J Biol Chem. 276, 27930-27935. PMID: 11359781
Protein-protein conjugate formationPDPH22301Backman C. et al. (1996) Systemic administration of a nerve growth factor conjugate reverses age-related cognitive dysfunction and prevents cholinergic neuron atrophy. J Neurosci. 16, 5437-5442. PMID: 8757256
Protein labelingPDPH22301Govindan S. V. et al. (1995) Site-specific modifications of light chain glycosylated antilymphoma (LL2) and anti-carcinoembryonic antigen (hImmu-14-N) antibody divalent f1agments. Cancer Res. 55, 5721s-5725s. PMID: 7493334
Protein-membrane protein interaction analysisPMPI28100Ozvegy-Laczka C. et al. (2008) Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter. J Biol Chem. 283, 26059-26070. PMID: 18644784
Nucleic acid immobilizationPMPI28100Shen G. et al. (2004) X-ray photoelectron spectroscopy and infrared spectroscopy study of maleimide-activated supports for immobilization of oligodeoxyribonucleotides. Nucleic Acids Res. 32, 5973-5980. PMID: 15537837
Protein immobilization onto chip or slidePMPI28100Chirayil S. et al. (2009) Discovering ligands for a microRNA precursor with peptoid microarrays. Nucleic Acids Res. 37, 5486-5497. PMID: 19561197
Nucleic acid immobilizationPMPI28100Pourmand N. et al. (2006) Direct electrical detection of DNA synthesis. Proc Natl Acad Sci U S A. 103, 6466-6470. PMID: 16614066
Protein-protein interaction analysisBMB22331Kida Y. et al. (2007) Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis. J Cell Biol. 179, 1441-1452. PMID: 18166653
Protein oligomerizationBMB22331Auclair J. R. et al. (2010) Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 107, 21394-21399. PMID: 21098299
Protein-membrane protein interaction analysisBMDB22332Steiner H. et al. (2008) Chemical cross-linking provides a model of the gamma-secretase complex subunit architecture and evidence for close proximity of the C-terminal fragment of presenilin with APH-1. J Biol Chem. 283, 34677-34686. PMID: 18801744
Protein-protein conjugate formationBMDB22332Batra-Safferling R. et al. (2006) Glutamic acid-rich proteins of rod photoreceptors are natively unfolded. J Biol Chem. 281, 1449-1460. PMID: 16280326
Protein oligomerizationBMDB22332Auclair J. R. et al. (2010) Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 107, 21394-21399. PMID: 21098299
Protein-protein interaction analysisBMDB22332Gavazzi F. et al. (2007) Wild-type opaque2 and defective opaque2 polypeptides form complexes in maize endosperm cells and bind the opaque2-zein target site. Plant Physiol. 145, 933-945. PMID: 17827273
in vivo protein crosslinkingBMOE22323Geula S. et al. (2012) Structure-based analysis of VDAC1 protein: defining oligomer contact sites. J Biol Chem. 287, 2179-2190. PMID: 22117062
Protein-protein interaction analysisBMOE22323Kida Y. et al. (2007) Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis. J Cell Biol. 179, 1441-1452. PMID: 18166653
Protein oligomerizationBMOE22323Auclair J. R. et al. (2010) Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 107, 21394-21399. PMID: 21098299
Protein oligomerizationBMOE22323Auclair J. R. et al. (2010) Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 107, 21394-21399. PMID: 21098299
Protein oligomerizationBMOE22323Auclair J. R. et al. (2010) Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 107, 21394-21399. PMID: 21098299
Protein oligomerizationBM(PEG)222336Auclair J. R. et al. (2010) Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 107, 21394-21399. PMID: 21098299
Protein oligomerizationBM(PEG)222336Auclair J. R. et al. (2010) Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 107, 21394-21399. PMID: 21098299
Protein-protein interaction analysisBM(PEG)222336Kida Y. et al. (2007) Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis. J Cell Biol. 179, 1441-1452. PMID: 18166653
Protein-protein interaction analysisBMH22330Kida Y. et al. (2007) Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis. J Cell Biol. 179, 1441-1452. PMID: 18166653
Protein oligomerizationBMH22330Beck R. et al. (2008) Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proc Natl Acad Sci U S A. 105, 11731-11736. PMID: 18689681
in vivo protein crosslinkingBMH22330Ahmad R. et al. (2012) The MUC1-C oncoprotein binds to the BH3 domain of the pro-apoptotic BAX protein and blocks BAX function. J Biol Chem. 287, 20866-20875. PMID: 22544745
Protein oligomerizationBMH22330Gogada R. et al. (2011) Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation. J Biol Chem. 286, 28749-28760. PMID: 21712378
Protein oligomerizationDTME22335Auclair J. R. et al. (2010) Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 107, 21394-21399. PMID: 21098299
in vivo protein crosslinkingDTME22335Smith A. L. et al. (2012) Association of Rho-associated protein kinase 1 with E-cadherin complexes is mediated by p120-catenin. Mol Biol Cell. 23, 99-110. PMID: 22031287
in vivo protein crosslinkingDTME22335Van Itallie C. M. et al. (2011) Claudin-2 forms homodimers and is a component of a high molecular weight protein complex. J Biol Chem. 286, 3442-3450. PMID: 21098027
Protein-protein interaction analysisDTME22335Zhang H. et al. (2010) The extreme C terminus of the ABC protein DrrA contains unique motifs involved in function and assembly of the DrrAB complex. J Biol Chem. 285, 38324-38336. PMID: 20876527
in vivo protein crosslinkingDTME22335Simonin A. and Fuster D. (2010) Nedd4-1 and beta-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis, and function. J Biol Chem. 285, 38293-38303. PMID: 20855896
Protein oligomerizationTMEA33043Auclair J. R. et al. (2010) Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 107, 21394-21399. PMID: 21098299
Protein oligomerizationTMEA33043Gosink K. K. et al. (2011) Mutational analysis of N381, a key trimer contact residue in Tsr, the Escherichia coli serine chemoreceptor. J Bacteriol. 193, 6452-6460. PMID: 21965562
Protein oligomerizationTMEA33043Studdert C. A. and Parkinson J. S. (2004) Crosslinking snapshots of bacterial chemoreceptor squads. Proc Natl Acad Sci U S A. 101, 2117-2122. PMID: 14769919