The alamarBlue Reagent is a non-toxic, water-soluble resazurin dye (a redox indicator) that yields a fluorescent signal and a colorimetric change when incubated with metabolically active cells. The reagent is designed for cell viability and proliferation studies using mammalian cell lines, bacteria and fungi and is ideally suited for in vitro cytotoxicity studies. Additionally, cell viability can be monitored in real time when performing a variety of tissue engineering applications.

Highlights:

• High sensitivity and linearity – detect as few as 50 cells
• Quantitative – accurate measurement over time
• Non-toxic to cells – low interference with metabolism
• Scalable – a mix-and-read, homogeneous assay
• No cell lysis – use with time-course or functional assays
• Stable – monitor continuous cell growth
• Flexible – cultured cells, bacteria and fungi
• Versatile – fluorescent or colorimetric detection

But you don’t have to take our word for it, because for a limited time you can obtain a free sample of this high-performance trypsin by completing a brief survey to describe how you use proteases in your research.

The Protein Methods Library now includes Western blot video tutorials that are all found on one page to give you a broad view of the procedure. The steps covered include:

• Separating proteins by SDS-PAGE
• Transferring proteins to the membrane
• Blocking the membrane to reduce background
• Probing the blot for your target protein
• Detecting your target proteins using chemiluminescent Western blot substrates
• Stripping the blot to reprobe it for another target protein

Thermo Scientific HisPur products are IMAC-based supports for his-tagged protein purification. These include magnetic beads and two kinds of agarose resin, cobalt and nickel varieties, and various sizes and formats of bottles and columns. HisPur Cobalt Superflow Agarose uses a proprietary cobalt-charged tetra-dentate chelator immobilized onto highly crosslinked (Superflow) 6% beaded agarose. In this article, our scientist present example purification data with HisPur Cobalt Superflow Agarose, which is especially suited for small- and large-scale purifications of recombinant poly-histidine tagged proteins by FPLC.

The triple luciferase reporter assay system uses Thermo Scientific Pierce Luciferase Assay Vectors and Kits (Gaussia, Cypridina, and Red Firefly luciferases). Choi et al. used the system with neuroblastoma cells (IMR-32) to test whether or not the parkinsonian neurotoxins paraquat, rotenone and MPP+ can activate ARE- and NF-κB-dependent transcription normalized by CMV-driven transcription. In so doing, the researchers demonstrate the practical application of this triple luciferase assay strategy to simultaneously monitor two pathways while normalizing to a third reporter of baseline activity.

These ELISA references are provided in an interactive table, so that you can search for specific target proteins, antibodies, disease states or authors and find not only the references but also a summary of how the authors used the respective ELISA kit, a link to the Pubmed listing of the article and a link to the product page of the kit.

Check out the ELISA References page!

The experiments compare sample dialysis performance with peptides and proteins of various sizes using Thermo Scientific Slide-A-Lyzer Devices and Thermo Scientific SnakeSkin Dialysis Tubing having 2K, 3.5K, 7K, 10K and 20K nominal MWCO ratings. The effect on dialysis rate of membrane surface-area versus sample-volume is also demonstrated.

Documentation systems for nucleic acid and protein gels and blots may have graduated from film to digital camera imaging, but lagging behind is development of easy-to-use software to analyze captured images: to autodetect bands, calculate molecular sizes, and quantitate relative abundances based on densitometry.

Welcome the new Thermo Scientific myImageAnalysis Software, a full-featured, multifunction computer program to analyze and edit digital images of electrophoresis gels and blots that were acquired using gel documentation imagers or scanners. It will expedite your day-to-day gel analysis work, but you don’t have to take our word for it. You can download the full-featured program to try for 30 days.

Highlights:

• Intuitive design – sleek task-bars organize functions into four main tabs
• Compatible file types – load and save images in popular file formats
• Image comparison – open two to six image files at once to review and edit
• Auto-identification – accurate, customizable, automatic lane and band identification
• MW determination – simple calculation of migration distances and molecular weights
• Advanced quantitation – auto-calculate purities and quantities of bands or lanes
• Image refinement and annotation – crop, straighten, invert, adjust, add notes
• Exporting and sharing – easily transfer data and images to Microsoft* Office* Programs

The new kit is just one of many useful protein and peptide sample preparation tools designed for proteomics mass spectrometry methods. See our MS Workflow Product Guide.

Highlights:

• Complete – includes all reagents, plus a digestion indicator, for 20 samples
• Simple – provides reproducible results even for non-expert MS analysts
• Flexible – can be adapted to handle sample sizes between 10 to 200μg
• High yield – total protein yield from 1 million cells is greater than 100μg
• Optimized – cysteine reduction and alkylation are 100%
• Efficient – percentage of missed cleavages is less than 10%
• Compatible – final preparation is ready for direct MS analysis

Highlights:

• Spectral grade – highly purified and tested for spectral clarity
• General solvents – acetonitrile, DMSO, DMF, pyridine, water
• Ion-pairing – HFBA, TFA, TEA, formic acid
• 1% solutions – TFA and formic acid in water and acetonitrile