All about HisPur Cobalt Superflow Agarose

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A new product article about HisPur Cobalt Superflow Agarose describes the purity, dynamic binding capacity, reusability and chemical compatibility properties of a new cobalt-charged IMAC resin.

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Dual assays? Try triple luciferase assays!

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A new product article about luciferase assay strategies describes how our researchers developed and demonstrated a method to simultaneously assay the expression of two different genes relative to a third (normalizing) gene.

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Example data explains dialysis membrane MWCO

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A new product article about the separation characteristics of dialysis membranes summarizes the results of various in-house experiments to explain and demonstrate the meaning of molecular weight cut-off (MWCO) specifications and rates of buffer exchange with dialysis tubing, cassettes and devices.

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Triple luciferase assays, cell imaging and neuron development

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A new product article, summarizes results on the use of three spectrally-resolvable luciferase reporters and high-content immunofluorescence cellular imaging to monitor phenotypic changes in neuronal precursor cell lines following retinoic acid-induced differentiation.

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Chromatography cartridge LC data

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A new product article on liquid chromatography columns presents results from various experiments that used selected desalting and affinity resins as supplied in the format of Thermo Scientific Pierce Chromatography Cartridges.

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New article on dual-spectral luciferase assays

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In this new product article, our researchers demonstrate how they performed experiments to confirm the efficacy of Thermo Scientific Pierce Luciferase Dual Assay Kits to spectrally resolve and quantify the activity of two luciferases using a commercial plate reader capable of simultaneous measurement at two different wavelengths.

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Phosphoprotein profiling by mass spectrometry

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This product article, republished from the 2009 volume of Previews magazine, describes the work of researchers who collaborated with our scientists to profile changes in phosphoprotein expression in cancer stem cells using several Thermo Scientific Instruments and Protein Biology Products.

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Generate stable isotope-labeled protein standards for quantitative mass spectrometry

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Stable isotope-labeled peptides are routinely used as internal standards for mass spectrometry (MS) quantitation of enzymatically digested protein samples. However, stable isotope-labeled proteins are considered better standards because they control for MS sample-preparation loss and digestion variations. In this Product Article about generating MS standards, our scientists describe using in vitro translation (IVT) as an

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Eliminate endotoxins from various protein sources, sizes and charges

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Traditional endotoxin removal methods require significant optimization for every target molecule being purified. Furthermore, ionic methods for binding endotoxins require pH optimization to avoid binding highly charged proteins, and size-exclusion methods to filter endotoxins are not effective for large proteins. In this Product Article about removing endotoxins from samples, our scientists demonstrate how they used

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Removing endotoxins from protein samples

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Endotoxin removal during the development of biological therapeutics is critical for preventing inflammation, tissue death and endotoxin shock. In this Product Article about removing endotoxins, our scientists discuss how they evaluated a spin-column method that is ideal for protein samples.

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