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Although plasmid-based luciferase assays are powerful tools for studying signal transduction pathways, cells with low transfection efficiency can make signal detection difficult. In this Product Article about performing luciferase assays in Jurkat cells, our scientists demonstrate the effectiveness of using the Thermo Scientific Pierce Luciferase Reporter Systems to transfect Jurkat cells and the Thermo Scientific
The study of protein localization and translocation is important for understanding protein functions and diseases, especially cancers. Subcellular protein fractionation enables protein localization discoveries, enhances detection of low-abundance species, reduces sample complexity and enables the monitoring of physiologic fluxes and redistribution under basal and stimulated/diseased conditions. Tissue samples, however, present an extra level of complexity
Synaptosomes are commonly used to study synaptic function because they contain functional ion channels, receptors, enzymes and proteins, as well as the intact molecular machinery for the release, uptake and storage of neurotransmitters. Signal transmission is highly regulated by neuronal phosphoproteins, and therefore, maintaining protein phosphorylation during synaptosome preparation is essential. In this Product Article
Proteases and phosphatases serve many metabolic and regulatory functions; however, upon cell lysis, the once compartmentally contained enzymes are capable of mass protein degradation and dephosphorylation.
Neurons have unique morphology and lipid content, making protein extraction from this cell type very challenging. In this Product Article about neuronal protein extraction, our scientists show how the Thermo Scientific N-PER Neuronal Protein Extraction Reagent enables total protein isolation from neuronal tissue or primary cultured cells without adversely affecting protein function.
Reporter gene assays are used to study complex cellular signaling pathways. In this Product Article about luciferase assays, our scientists demonstrate how dual-spectral luciferase assay kits can be used to monitor NFkB and CRE promoter activity.
Given the many and various uses of antibodies in laboratory research endeavors, it’s no surprise that there are many available sizes and varieties of affinity purification columns, chromatography cartridges, filter plates and other devices for purifying antibodies easily and quickly at small or large scales. |
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