Improved MEF primary cell isolation

Product-Articles-Art-250

In a new product article, Dr. Hai-Yan Wu and colleagues compare available protocols for mouse embryonic fibroblast (MEF) isolation, showcasing the newly developed Pierce MEF Isolation Kit.

Continue reading…

Quantitative IP in a high-throughput format

Product-Articles-Art-250

In a new product article, Dr. Jae Choi and colleagues describe key features of the Pierce qIP Protein Interaction Assay System and then provide example experimental data and demonstrate its use in a high-throughput (HTS) microplate format.

Continue reading…

Isolate and culture primary cardiomyocytes easily and speedily

Product-Articles-Art-250

In a new product article, Dr. Hai-Yan Wu and colleagues compare available protocols for primary cardiomyocyte isolation from mouse and rat hearts, showcasing the newly developed Pierce Cardiomyocyte Isolation Kit.

Continue reading…

No Joke! Express glycoproteins in a human IVT system

Product-Articles-Art-250

In a new product applications article, Krishna Vattem provides a protocol and demonstrates how the 1-Step Human Coupled in vitro Translation System can be used to express glycoproteins complete with N-linked glycosylation.

Continue reading…

Comparing desalting columns for protein recovery performance

Product-Articles-Art-250

In a new product applications article, we compared small and large sizes of Thermo Scientific Zeba Spin Desalting Columns with closest equivalent columns from two other manufacturers for desalting performance with pure-protein and cell-lysate samples.

Continue reading…

How many antibody molecules per agarose bead?

Product-Articles-Art-250

Quick! Guess how many molecules of antibody (IgG) can be bound per bead of Protein A/G agarose resin:

Continue reading…

Complete data on a complete bacterial protein extraction reagent

Product-Articles-Art-250

In a new article, staff scientists Eglė Namavičiūtė and colleagues present product development data to demonstrate performance of an improved formulation of B-PER Bacterial Protein Extraction Reagent.

Continue reading…

We’ll help you isolate and culture primary neurons, if you’ve got the brains for it!

Product-Articles-Art-250

In a new product article, Hai-Yan Wu and colleagues present results from product validation experiments with the new Thermo Scientific Pierce Primary Neuron Isolation Kit.

Continue reading…

Highly active proteins expressed by IVT

Product-Articles-Art-250

A new product article demonstrates the ability of the Thermo Scientific 1-Step Human High-Yield In Vitro Translation Kit to rapidly express highly functional caspase-3 enzyme, as well as how the system can be used to screen modulators of protein activity.

Continue reading…

In vitro protein expression in a 96-well format

Product-Articles-Art-250

A new product article demonstrates how to use 96-well microdialysis plates to perform high-yield in vitro translation (IVT), also called cell-free protein expression, with Thermo Scientific Human High-Yield Mini IVT Kits.

Continue reading…