Western blot infographic workflow guide

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Our new Western Blot Workflow and Product Guide is a no-nonsense, single-screen infographic that summarizes the key steps of Western blot analysis. The complete workflow diagram provides quick links to Thermo Scientific Pierce Protein Biology Products

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Are imagers really better than X-ray film for Western blots?

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In a new product applications article, research developers of the Thermo Scientific myECL Imager demonstrate that cooled-CCD camera imaging technologies have surpassed traditional photographic X-ray film for detection and quantitative analysis of chemiluminescent Western blots.

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Quick! Transfer to this new semi-dry blotter!

Blotting Equipment, Film and Accessory Reagents

Try the new Thermo Scientific Pierce G2 Fast Blotter, designed specifically for rapid 5-minute (or traditional 1-hour) semi-dry transfer of all sizes of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 10 minutes.

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Western Blot Video Tutorials now available

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Do you need to run a Western blot but can’t quite understand how to do it? Now you can watch videos that demonstrate how to do each step in the Western blot process and learn why each step is performed.

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Try our gel and blot image analysis software

Blotting Equipment, Film and Accessory Reagents

Finally, you can give away that ruler and permanent marker you’ve kept for measuring and calculating band molecular weights!

Documentation systems for nucleic acid and protein gels and blots may have graduated from film to digital camera imaging, but lagging behind is development of easy-to-use software to analyze captured images: to autodetect bands, calculate molecular

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New clear milk blocking buffer

Blocking Buffers for Protein Methods

Looking for a more stable, uniform milk-based blocking solution? Try the new Thermo Scientific Pierce Clear Milk Blocking Buffer. This 10X concentrated solution is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.

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Ultra-colorimetric Western blot detection

Western Blotting Substrates

The new 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

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A better way to detect S-nitrosylation

Post-Translational Modification Enrichment Kits

Try our new Pierce S-Nitrosylation Western Blot Kit to detect protein S-nitrosocysteine post-translational modifications. This kit improves upon the original biotin-switch method developed by Jeffrey, et al., which used biotin-HPDP to label S-nitrosylated proteins for subsequent Western blot detection via streptavidin-HRP conjugate.

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myECL Imager tutorial videos

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Three videos that demonstrate how to use the Thermo Scientific myECL Imager to capture your data are now available.

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Looking to improve your image?

Protein Electrophoresis and Staining

Improve your Western blot and stained gel imaging with the Thermo Scientific myECL Imager, an easy-to-use instrument for sensitive, multimode image capture and analysis via an intuitive touchscreen interface and advanced integrated software.

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